A Novel Immunoassay for the Quantization of CYFRA 21–1 in Human Serum

He, An; Liu, Tiancai; Dong, Zhi-Ning; Ren, Zhi-Qi; Hou, Jingyuan; Li, Ming; Wu, Yixuan

doi:10.1002/jcla.21597

Cited by 28 publications

(22 citation statements)

References 26 publications

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“…In addition, Several methods have been reported for simultaneous determination of mixture of these active ingredients like UV spectrophotometry [23,24], electrochemical method [25][26][27], chemiluminescence [28], capillary electrophoresis [29][30][31][32], high-performance liquid chromatography (HPLC) [33][34][35][36]and NIR FTIR [37]. The objective of this study is to develop a simple, rapid, sensitive and selective method for determination of rutin and ascorbic acid mixture in their pure forms and pharmaceutical dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

Attia

2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

Attia

2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Next, 10 µL of blocking buffer was added for 1 hour at 37°C to block unreacted aldehyde sites, washed three times with washing buffer by centrifugation at 20,000× g for 10 minutes, and resuspended in phosphate buffer solution containing 0.05% Proclin-300. 23 The functional acceptor beads were stored at 4°C.…”

Section: Coupling Of Anti-pct Monoclonal Antibodies (Ab2) To Acceptormentioning

confidence: 99%

Establishment of a novel homogeneous nanoparticle-based assay for sensitive procalcitonin detection of ultra low-volume serum samples

Chen¹,

Liu²,

Li³

et al. 2018

IJN

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PurposeSepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Although C-reactive protein (CRP), interleukin-6 (IL-6), and procalcitonin (PCT) might be biomarkers for sepsis diagnosis, PCT is more sensitive and specific than CRP or IL-6. We aimed to establish an efficient immunoassay that precisely detects PCT in human serum for the early diagnosis of sepsis.Materials and methodsWe developed a novel amplified luminescent proximity homogeneous assay (AlphaLISA) for the quantitative detection of PCT in serum. In this assay, a pair of antibodies was used to capture PCT in serum and to form sandwich complexes after incubating for 15 minutes at 37°C.ResultsPCT concentrations were determined within a linear range of 0.016–100 ng/mL. The limit of detection was 18.6 pg/mL. The results demonstrate that the reproducibility, recovery, and specificity of this assay for PCT meet the requirements of clinical detection. The coefficient of determination (R2) between this method and commercially available enzyme-linked fluorescent assay (ELFA) kits was estimated to be 0.93045 in clinical serum testing.ConclusionThe novel assay for PCT detection was robust with high sensitivity and a broad dynamic range. Compared with conventional heterogeneous detection methods such as ELISA, this assay measured the concentration of the homogeneous form of PCT and provided results that are more accurate within a shorter detection time. We expect that this novel method will be useful for the early screening and prognosis evaluation of patients with sepsis.

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“…Their molecular weights may explain why CYFRA 21-1 has a greater molar concentration than AFP (i.e., 40 kDa for CYFRA 21-1; (33) 70 kDa for AFP (37) ). Moreover, the second reason may be the different sizes of CYFRA 21-1 (estimated to be equivalent to a sphere with a diameter of 4.5 nm (33) ) and AFP (5 × 5 × 5 nm 3 (38) ). In addition, the adsorption of the AFP molecules onto the antibody-immobilized surface might be limited by steric hindrance compared with the smaller CYFRA 21-1, resulting in a decrease in the intensity of effective signals.…”

Section: Concentration-dependent Detection Of Cancer Biomarkers Usingmentioning

confidence: 99%

Multianalyte Detection of Cancer Biomarkers in Human Serum Using a Label-Free Field Effect Transistor Biosensor

Cheng

Hideshima³

et al. 2018

Sensors and Materials

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Recent statistics show that the incidence and mortality of cancer are on the rise. Among all types of cancer, lung cancer and liver cancer are the most prevalent. We have many biomarkers for these two cancers, among which the cytokeratin fragment 21-1 (CYFRA 21-1) and α-fetoprotein (AFP) are the most common and widely used. Among the various detection methods, the field effect transistor (FET) biosensor is one of the most attractive approaches, providing a label-free, fast, and low-cost electrical detection of biomarkers with high specificity and sensitivity. In previous work, we applied biomarkers for a single cancer type. With the detection of CYFRA 21-1 and neuron-specific enolase (NSE), lung cancer was differentiated in the early stages. Compared with the early work, the biomarkers for the two cancer types, CYFRA 21-1 for lung cancer and AFP for liver cancer, were prepared in this work. The detection of CYFRA 21-1 and AFP in human serum was achieved at the same time with the limits of detection of 1 and 10 ng mL −1 , while the cut-off values were 4 and 10 ng mL −1 . The method featured short analytical time, small sample volume, and low cost. With its good selectivity and appropriate sensitivity, clinical application will become popular in the future.

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A Novel Immunoassay for the Quantization of CYFRA 21–1 in Human Serum

Abstract: The AlphaLISA CYFRA 21-1 kit developed in this study had favorable performance characteristics for clinical application with acceptable analytical sensitivity, specificity, and accuracy.

Cited by 28 publications

References 26 publications

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

Establishment of a novel homogeneous nanoparticle-based assay for sensitive procalcitonin detection of ultra low-volume serum samples

Multianalyte Detection of Cancer Biomarkers in Human Serum Using a Label-Free Field Effect Transistor Biosensor

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