A Novel Improved Gram Staining Method Based on the Capillary Tube

Wu, Kong-Yang; Yang, Tao

doi:10.33073/pjm-2020-043

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…Gram staining is an essential technique in microbiology. It was invented by Danish bacteriologist Hans Christian Gram in 1882 ( Wu and Yang 2020 ). Gram staining distinguishes Gram-positive from Gram-negative organisms based on differences in their cell walls.…”

Section: Methodsmentioning

confidence: 99%

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Duan

Wang

2022

Polish Journal of Microbiology

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Staphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect S. aureus. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple methods for diagnosing S. aureus was performed. The following methods were included: (a) Hybrisep in situ hybridization test, (b) T2Dx system, (c) BinaxNow Staphylococcus aureus and PBP2a, (d) Gram staining, (e) PNA FISH and QuickFISH, (f) Accelerate PhenoTM system, (g) MALDI-TOF MS, (h) BioFire FilmArray, (i) Xpert MRSA/SA. These rapid and simple methods can rapidly identify S. aureus in positive blood cultures or direct blood samples. Furthermore, BioFire FilmArray and Xpert MRSA/SA identify methicillin-resistant S. aureus (MRSA), and the Accelerate PhenoTM system can also provide antimicrobial susceptibility testing (AST) results. The rapidity and simplicity of results generated by these methods have the potential to improve patient outcomes and aid in the prevention of the emergence and transmission of MRSA.

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Section: Methodsmentioning

confidence: 99%

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Duan

Wang

2022

Polish Journal of Microbiology

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“…Gram difference in bacteria was shown from the purple staining for Gram-positive and red staining for Gram-negative. Beveridge (2001) and Wu and Yang (2020) stated that Gram staining was used to clarify whether Gram-positive and Gram-negative bacteria were. Gram staining is used for initial clarification in unknown isolates.…”

Section: Isolate Gram Staining and Morphological Observationmentioning

confidence: 99%

Identification of lipase producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia

Layly

Meryandini

Helianti³

et al. 2021

Biodiversitas

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Abstract. Layli IR, Meryandini A, Helianti I, Astuti RI. 2021. Identification of lipase-producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia. Biodiversitas 22: 4512-4524. Lipase (triacylglycerol hydrolase E.C.3.1.1.3) is an enzyme that catalyzes the hydrolysis reaction of triacylglycerol into free fatty acids and glycerols. Lipase has broad application spectrum and unique catalytic mechanism, as one of important enzymes for industries, and almost all enzymes used for industries are originated from microbes. Identification of lipase-producing bacteria from palm oil processing sewage sludge was carried out to determine the potential of isolates and the application of lipase enzymes for industry. This study identified lipase-producing bacteria from the screening and isolation results from palm oil sewage sludge in Malimping-Banten. Two isolates were obtained from screening and isolation results. The morphological and observation and Gram staining method resulted in mlp-1 colony isolate was Gram-negative with basil cell shape, while mlp-2 colony isolate was Gram-positive with basil cell shape. Qualitative test on two isolates in tributyrin agar showed that two isolates produced clear zone or lipase enzyme. Molecular identification using 16SrRNA sequence showed that mlp-1 colony isolate was Chryseobacterium gleum, while mlp-2 colony was Bacillus velezensis. Literature study stated that Chryseobacterium gleum are pathogenic bacteria, resulting in uncontinued identification process, while Bacillus velezensis are non-pathogenic bacteria that have potential for industrial application. Identification of Bacillus velezensis was continued by performing biochemical test and inhibitory test. The continued identification results supported the molecular identification of lipase-producing enzyme for Bacillus velezensis.

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Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Parasuraman,

Busi,

Lee

2024

ESKAPE Pathogens

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A Novel Improved Gram Staining Method Based on the Capillary Tube

Cited by 4 publications

References 27 publications

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Identification of lipase producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia

Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

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