A novel in vivo model for rapid evaluation of Aliivibrio salmonicida infectivity in Atlantic salmon

Kashulin, Alexander; Sørum, Henning

doi:10.1016/j.aquaculture.2013.10.025

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…Kashulin and Sorum () developed a new, rapid and cost‐effective model for studying Cold‐Water Vibriosis (CWV) (Kashulin & Sorum, ). No matter which immersion groups the Atlantic salmon fry were assigned, only the amount of Vibrio salmonicida in the blood samples was detected.…”

Section: Discussionmentioning

confidence: 99%

“…In many quantitative analysis of pathogens, the detection of pathogens relied upon plate cultivation (Farto et al, 2011;Kashulin & Sorum, 2014;Liu et al, 2015). Compared with this traditional method, qPCR used here was rapid, economical, specific and sensitive for the quantitative detection of AS-C4, with just a detection limit of 5.6 copies of one PCR reaction (Du, Liu, et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…It is the first investigation of routes of ASM infection in Atlantic salmon, and the first sites of ASM infection will provide information for the development of new therapeutic strategies to protect against this pathogen in salmon aquaculture. Kashulin and Sorum (2014) developed a new, rapid and cost-effective model for studying Cold-Water Vibriosis (CWV) (Kashulin & Sorum, 2014). No matter which immersion groups the Atlantic salmon fry were assigned, only the amount of Vibrio salmonicida in the blood samples was detected.…”

Section: Discussionmentioning

confidence: 99%

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Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Liu

Meng

et al. 2018

Aquac Res

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Aeromonas salmonicida subsp. masoucida (ASM) is classified as atypical A. salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS‐C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salar L.) by an immersion challenge with the control group (T0, no AS‐C4), group T1 (2.67 × 104 CFU/ml AS‐C4) and group T2 (2.67 × 107 CFU/ml AS‐C4). The numbers of AS‐C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real‐time PCR (qRT‐PCR). AS‐C4 were detected in the gill and intestine as early as 0 hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS‐C4 into salmon. Although AS‐C4 could not be detected in the skin until 24 hr after the challenge in T1 group, it could be detected in the skin as early as 0 hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS‐C4 into salmon. AS‐C4 was immediately detected in the blood within 3 hr after it entered the host, suggesting that AS‐C4 successfully invaded the bloodstream of fish. After AS‐C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Liu

Meng

et al. 2018

Aquac Res

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“…The data of Brattgjerd and Evensen have demonstrated that antigens of A. salmonicida remain detectable in the phagosomes of head kidney macrophages of Atlantic salmon only up to 27 h post‐infection (Brattgjerd & Evensen ). At the same time, the question on the portal of the infection was open until 2014 when Kashulin and Sørum described the intact salmon skin as a portal of the infection (Kashulin & Sørum ). The new findings on the portal of CWV correlate well with the earlier findings of Bøgwald et al .…”

Section: Pathogenesismentioning

confidence: 99%

“…), A. salmonicida rapidly enters the Atlantic salmon blood stream via intact skin and already from the first minutes post‐challenge become detectable by standard cultivation on blood agar plates. The bacterium not only requires a short invasion time, but also enters the host in extremely high numbers reaching concentrations up to 1 × 10 6 CFU × mL −1 of blood after 3 min of exposure (Kashulin & Sørum ). After successful invasion into the host tissues, A. salmonicida seems to utilize general muting of the gene expression to avoid the host immune system (Bjelland et al .…”

Section: Pathogenesismentioning

confidence: 99%

Cold‐water vibriosis. The current status of knowledge

Kashulin

Seredkina

Sørum

2016

Journal of Fish Diseases

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The current review for the first time summarizes the findings of the 30 years of research on cold-water vibriosis (CWV). The diseased caused by Aliivibrio salmonicida (earlier known as Vibrio salmonicida) was for the first time described in 1986 and became one of the most important bacterial diseases in salmon aquaculture. The lack of appropriate vaccine hampered development of Atlantic salmon aquaculture until the late 1980s when a novel vaccine allowed dramatic increase in the Atlantic salmon farming. In December 2007, the genus Vibrio was split into two genera and several bacterial species including V. salmonicida were transferred to genus Aliivibrio. The change of the names create significant difficulties with the designation of the CWV disease agent since its abbreviation A. salmonicida became similar to another well-known salmon pathogen Aeromonas salmonicida (A. salmonicida). The disease was considered as controlled by vaccination, but reappeared at Atlantic salmon farms in 2011, this time affecting vaccinated Atlantic salmon. The current review summarizes the knowledge on pathogenesis, vaccination and treatment of CWV and proposes further directions for studying the disease.

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Methods for Measuring Efficacy, Safety and Potency of Fish Vaccines

Midtlyng

2016

Fish Vaccines

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A novel in vivo model for rapid evaluation of Aliivibrio salmonicida infectivity in Atlantic salmon

Cited by 11 publications

References 43 publications

Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Cold‐water vibriosis. The current status of knowledge

Methods for Measuring Efficacy, Safety and Potency of Fish Vaccines

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