A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants

Jäckel, Susanne; Eiden, Martin; Balkema‐Buschmann, Anne; Ziller, Mario; Vuren, Petrus Jansen van; Pawęska, Janusz T.; Groschup, Martin H.

doi:10.1016/j.rvsc.2013.04.015

Cited by 24 publications

(21 citation statements)

References 21 publications

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“…In our study, sera from livestock were tested for viremia by real‐time qRT‐PCR and run in two commercially available ELISAs. Moreover, small ruminant sera were also tested in a newly developed indirect IgG ELISA (Jäckel et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…All samples from sheep and goats were tested in an in‐house indirect IgG ELISA based on bacterially expressed ΔGn protein. Production of recombinant antigen and the assay procedure was carried out as described previously (Jäckel et al., ). Maxisorb immunoplates (Nunc, Roskilde, Denmark) were coated with recombinant RVFV ΔGn protein, diluted in 0.05 m carbonate–bicarbonate buffer (pH 9.6) to a concentration of 2 μg/ml (100 μl diluted antigen per well).…”

Section: Methodsmentioning

confidence: 99%

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Molecular and Serological Studies on the Rift Valley Fever Outbreak in Mauritania in 2010

Jäckel

Eiden

Mamy³

et al. 2013

Transbound Emerg Dis

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SummaryRift Valley fever virus (RVFV) is a vector-borne RNA virus affecting humans, livestock and wildlife. In October/November 2010, after a period of unusually heavy rainfall, a Rift Valley fever outbreak occurred in northern Mauritania causing clinical cases in cattle, sheep, goats and camels, 21 of which were of lethal outcome. The aim of this study was to obtain further information on the continuation of RVF virus activity and spread in animal species in Mauritania after this outbreak. We therefore tested sera from small ruminants, cattle and camels for the presence of viral RNA and antibodies against RVFV. These sera were collected in different parts of the country from December 2010 to February 2011 and tested with three different ELISAs and an indirect immunofluorescence assay. The results show a high seroprevalence of RVFV IgM and IgG antibodies of about 57% in all animals investigated. Moreover, in four camel sera, viral RNA was detected emphasizing the important role camels played during the latest RVF outbreak in Mauritania. The study demonstrates the continuous spread of RVFV in Mauritania after initial emergence and highlights the potential role of small ruminants and camels in virus dissemination.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular and Serological Studies on the Rift Valley Fever Outbreak in Mauritania in 2010

Jäckel

Eiden

Mamy³

et al. 2013

Transbound Emerg Dis

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“…In this study, the seroprevalence of RVF was investigated among human population in Borno State, Nigeria to determine the occurrence and spread of the disease in comparison with those of Lassa fever and CCHF. Since recombinant protein-based immunoassays with high sensitivity and specificity have been demonstrated to be useful for the diagnosis of VHFs in humans, [15][16][17]25,26 we have used rNP-based IgG-ELISAs for the detection of serum antibodies against RVFV, LASV, and CCHFV. Of the 297 serum samples tested for RVFV-IgG, a total of 42 (14.1%) showed positive results ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of serological assays for viral hemorrhagic fevers and determination of the prevalence of Rift Valley fever in Borno State, Nigeria

Bukbuk

Fukushi

Tani

et al. 2014

Transactions of the Royal Society of Tropical Medicine and Hygiene

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“…Many lab methods have been used to diagnose RVF infection. However, in performed studies, ELISA technique has were proved to have been highly sensitive in herd scanning compared to other serological tests [15,17]. Scott et al [17] detected specificity and sensitivity rate of ELISA test as 100% in serological testing of RVF infection.…”

Section: Discuss the Implication Of Distribution Of Positivitymentioning

confidence: 99%

“…In diagnosing RVF infection, serological tests such as virus neutralization, hemagglutination inhibition (HAI), plaque reduction neutralization (PRN), complement fixation (CF), indirect immunofluorescence assay (IFA) and ELISA can be used as well as virological and molecular methods [14,15,16,17].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Rift Valley Fever Virus Infection Serologically and Pathologically in Aborted Cattle, Sheep, Goats and in Fetuses

Kale

Hasırcıoğlu

Özmen

et al. 2020

ARRB

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In this study, Rift Valley Fever Virus (RVFV) infection was searched serologically and pathologically in cattle (178 Holstein), sheep (160 native), goats (66 ordinary goats, 98 Honamli goats, 16 Saanen goats) with an abortion history and in unborn cattle (8), sheep (24) and goat (5) fetus. Samples were collected between July 2009 and September 2010. As a result of studying specific antibodies to RVFV by using the c-ELISA method in blood serum samples collected from cattle, sheep and goats suffering abortion, seropositivity was identified in 7 cattle (7/178; 3.93%), 4 sheep (4/160;2.50%) and 18 goats (18/180;10.0%). 18 seropositive goats were distributed according to race as 13 ordinary goats (19.70%), 2 Honamli goats (2.04%) and 3 Saanen goats (18.75%). When liver, spleen and brain samples of the unborn fetus of cattle, sheep and goats were studied histopathologically, no pathological findings on RVFV disease were obtained. Consequently, in this study, where RVFV infection in cattle, sheep and goats raised in Western Mediterranean Region of Turkey was serologically revealed, it was concluded that RVFV did not take place in the aetiology of abortion cases in relevant species.

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A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants

Cited by 24 publications

References 21 publications

Molecular and Serological Studies on the Rift Valley Fever Outbreak in Mauritania in 2010

Molecular and Serological Studies on the Rift Valley Fever Outbreak in Mauritania in 2010

Development and validation of serological assays for viral hemorrhagic fevers and determination of the prevalence of Rift Valley fever in Borno State, Nigeria

Investigation of Rift Valley Fever Virus Infection Serologically and Pathologically in Aborted Cattle, Sheep, Goats and in Fetuses

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