A novel ionic amphiphilic chitosan derivative as a stabilizer of nanoemulsions: Improvement of antimicrobial activity of Cymbopogon citratus essential oil

Bonferoni, Maria Cristina; Sandri, Giuseppina; Rossi, Silvia; Usai, Donatella; Liakos, Ioannis; Garzoni, Alice; Fiamma, Maura; Zanetti, Stefania Anna Lucia; Athanassiou, Athanassia; Caramella, Carla; Ferrari, Franca

doi:10.1016/j.colsurfb.2017.01.043

Cited by 53 publications

(39 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chitosan concentration was studied in the range 0.1% ( w / v ) slightly higher than the maximum one considered in Phase 1, and 0.2% ( w / v ), that in a previous work allowed the authors to obtain nanoemulsions of a few hundred nanometers in size [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…If a derivative of a bioactive polymer is used, some of the polymer properties can be maintained by the nanoemulsion. Recently, some evidence was given of the ability of a new amphiphilic chitosan salt, chitosan oleate CS-OA [ 18 ], obtained by ionic interaction between chitosan and oleic acid, to stabilize an essential oil nanoemulsion [ 19 ]. It was observed that a low energy method could be combined with the self-assembling behavior of chitosan after electrostatic interaction with the hydrophobic moieties of oleic acid.…”

Section: Introductionmentioning

confidence: 99%

“…Nanoemulsions of dimensions in the nanometric range were obtained, depending on chitosan concentration and resulted physically stable for at least three months. The zeta potential of the nanoemulsion confirmed that the chitosan derivative was adsorbed at the droplet interface thanks to affinity of oleic chains for hydrophobic phase, while chitosan backbone resulted arranged towards the aqueous medium [ 19 ]. Chitosan oleate maintained in this case an antimicrobial effect, in accordance with literature findings [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

et al. 2018

Self Cite

View full text Add to dashboard Cite

Most of the methods of poly-lactic-glycolic acid (PLGA) preparation involve the passage through the emulsification of a PLGA organic solution in water followed by solvent evaporation or extraction. The choice of the droplet stabilizer during the emulsion step is critical for the dimensions and the surface characteristics of the nanoparticles (NPs). In the present work, a recently described ionic amphiphilic chitosan derivative, chitosan oleate salt (CS-OA), was proposed for the first time to prepare PLGA NPs. A full factorial design was used to understand the effect of some formulation and preparation parameters on the NP dimensions and on encapsulation efficiency (EE%) of Nile red, used as a tracer. On the basis of the DoE study, curcumin loaded NPs were prepared, having 329 ± 42 nm dimensions and 68.75% EE%. The presence of a chitosan coating at the surface was confirmed by positive zeta potential and resulted in mucoadhesion behavior. The expected improvement of the interaction of the chitosan surface modified nanoparticles with cell membrane surface was confirmed in Caco-2 cell culture by the internalization of the loaded curcumin.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Stapf ( Lemongrass ), thus improving its delivery. The combination of spontaneous emulsification process with chitosan oleate amphiphilic properties resulted in: (i) stable dispersion of a few hundred nanometer droplets; (ii) maintenance or improvement in the essential oil antimicrobial activity towards nine bacterial and ten fungal strains; (iii) biocompatibility of the nanoemulsion (cytotoxicity test performed on four different cell lines) [ 76 ].…”

Section: Spectrum Of Activitymentioning

confidence: 99%

Update on Monoterpenes as Antimicrobial Agents: A Particular Focus on p-Cymene

et al. 2017

View full text Add to dashboard Cite

p-Cymene [1-methyl-4-(1-methylethyl)-benzene] is a monoterpene found in over 100 plant species used for medicine and food purposes. It shows a range of biological activity including antioxidant, anti-inflammatory, antinociceptive, anxiolytic, anticancer and antimicrobial effects. This last property has been widely investigated due to the urgent need for new substances with antimicrobial properties, to be used to treat communicable diseases whose diffusion in developed countries has been facilitated by globalization and the evolution of antimicrobial resistance. This review summarizes available scientific data, as reported by the most recent studies describing the antimicrobial activity of p-cymene either alone, or as the main component of plant extracts, as well as addressing the mechanisms of action of cymenes as antimicrobial agents. While p-cymene is one of the major constituents of extracts and essential oils used in traditional medicines as antimicrobial agents, but considering the limited data on its in vivo efficacy and safety, further studies are required to reach a definitive recommendation on the use and beneficial effects of p-cymene in human healthcare and in biomedical applications as a promising candidate to functionalize biomaterials and nanomaterials.

show abstract

“…In a recent study, a stimulating effect of αTphNE on fibroblast and keratinocyte proliferation was observed [ 27 ]. Previous studies demonstrated moreover that chitosan oleate itself has some biological activities useful to application in wounds, such as keratinocyte stimulation and anti-infective activity [ 27 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds

et al. 2018

Self Cite

View full text Add to dashboard Cite

Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds. Preliminary studies in vitro on fibroblast cell cultures demonstrated good compatibility of platelet lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the platelet lysate application to wounds.

show abstract

A novel ionic amphiphilic chitosan derivative as a stabilizer of nanoemulsions: Improvement of antimicrobial activity of Cymbopogon citratus essential oil

Cited by 53 publications

References 33 publications

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

Update on Monoterpenes as Antimicrobial Agents: A Particular Focus on p-Cymene

Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds

Contact Info

Product

Resources

About