A novel, label-free fluorescence detection method for the formation of G-quadruplex DNA based on DNA-templated silver nanoclusters

Abstract

A novel, label-free fluorescence detection method was proposed for the formation of the G-quadruplex DNAs based on placing two DNA-Ag NCs together to light-up fluorescence.

Recent Developments in G-Quadruplex Binding Ligands and Specific Beacons on Smart Fluorescent Sensor for Targeting Metal Ions and Biological Analytes

“…These nanomaterials not only bind to G4 for the detection of metal ions and biomolecules but are also used for the recognition of G4. In 2017, Wei et al designed a system for detecting the formation of a G4 based on the principle of two dark DNA/Ag NCs together for light-up . The sensitivity depends on the distance between the 3′ and 5′ ends when the sensor forms a G4; the closer the 3′ and 5′ terminals, the higher the sensitivity.…”

“…In 2017, Wei et al designed a system for detecting the formation of a G4 based on the principle of two dark DNA/Ag NCs together for light-up. 222 The sensitivity depends on the distance between the 3′ and 5′ ends when the sensor forms a G4; the closer the 3′ and 5′ terminals, the higher the sensitivity. In 2015, Yang et al developed a "turnoff-on" approach using ZnCdSe QDs as the fluorescent probe to investigate the interaction between the G4 and TMPyP (Figure 12D).…”