A novel leukodepletion filter from electrospun poly(ethylene-vinyl alcohol) membranes and evaluation of its efficiency

Mayuri, P V; Bhatt, Anugya; Sabareeswaran, A; Ramesh, P.

doi:10.1080/00914037.2015.1099101

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…Our strategy was to graft SMDB onto electrospun EVAL by UV initiation. It was already proven in our previous study that, electrospun EVAL is a potential membrane material for the fabrication of leukodepletion filters; however, the extent of platelet adhesion to membranes has to be reduced …”

Section: Discussionmentioning

confidence: 96%

“…In addition to this, it is a biocompatible material used for several biomedical applications and an excellent membrane material that is useful for hemodialysis, separation of proteins, hemoglobin, uremic toxins, and so on . In our previous study, electrospun EVAL membranes have been pinpointed to have the ability to be used as membranes in leukodepletion filters . We inferred from the study that EVAL fibroporous membranes could efficiently remove leukocytes while retaining all other blood components except platelets from whole blood.…”

Section: Introductionmentioning

confidence: 99%

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Sulfobetaine‐functionalized electrospun poly(ethylene‐co‐vinyl alcohol) membranes for blood filtration

Mayuri

Bhatt

Ramesh

2018

J of Applied Polymer Sci

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Functionalization imparting zwitterionic sulfobetaines has been proven as the most versatile method for improving the hemocompatibility of polymers. In this study, we aimed to enhance the hemocompatibility of electrospun poly(ethylene‐co‐vinyl alcohol) (EVAL) by photografting with a betaine N‐(3‐sulfopropyl)‐N‐methacroyloxyethyl‐N,N‐dimethyl ammonium betaine (SMDB). SMDB was UV‐photografted to electrospun EVAL fibroporous membranes to obtain EVAL‐g‐PSMDB poly[N‐(3‐sulfopropyl)‐N‐methacroyloxyethyl‐N,N‐ dimethylammonium betaine] grafted on EVAL with different extents of grafting. The functionalization was confirmed by analysis of the attenuated total reflectance–Fourier transform infrared spectra. The effects of functionalization on the morphology, wettability, mechanical properties, and hemocompatibility of the electrospun EVAL membranes were also studied by scanning electron microscopy, water contact angle measurement, universal testing machine measurement, and in vitro hemocompatibility evaluation, respectively. The findings highlight that SMDB functionalization significantly reduced protein adsorption, hemolysis, and platelet adhesion. Blood cell consumption studies projected that the SMDB‐functionalized EVAL was able to capture leukocytes from blood, and hence, this system has the potential to be used as a filter medium for the selective removal of leukocytes from blood. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47057.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Sulfobetaine‐functionalized electrospun poly(ethylene‐co‐vinyl alcohol) membranes for blood filtration

Mayuri

Bhatt

Ramesh

2018

J of Applied Polymer Sci

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“…The behavior of BSA-immobilized EVAL membrane surface at the interface with blood was established by the in vitro hemocompatibility evaluation following the (2020) 2:1738 | https://doi.org/10.1007/s42452-020-03515-2 filter housing and further analyzed by SEM and histological examinations. The methods of sample processing for these two examinations have been reported elsewhere [15]. All these tests under in vitro hemocompatibility evaluation and whole blood filtration were done using human blood collected from healthy voluntary donor with informed consent, and the studies have been approved by the Institutional Ethics Committee of Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCT/IEC/594/2014 dated 21/04/2014).…”

Section: Analysis Of Blood-materials Interactions and Leukodepletion Ementioning

confidence: 99%

“…Herein we delineate an attempt for the betterment of hemocompatibility and leukodepletion efficiency of electrospun poly(ethylene-co-vinyl alcohol) (EVAL) membranes by covalent immobilization of bovine serum albumin (BSA) via polydopamine spacer. In spite of the competence in leukocyte removal, electrospun EVAL membranes disbenefit from high levels of platelet adhesion, which entails further contemplation [15]. Notwithstanding the fact that albumin is effective in repelling the platelets, membrane bound with albumin has not yet been investigated for their leukodepletion performance.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the potency of leukodepletion filter membranes immobilized with bovine serum albumin via polydopamine spacer

2020

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Conjugation of biomolecules over membrane surfaces intended for blood contacting applications has been proven worthwhile for the improvement of their hemocompatibility. In conjunction with this factuality, this manuscript for the first time unveils the feasibility of bovine serum albumin (BSA) immobilization via polydopamine precursors on electrospun poly(ethylene-co-vinyl alcohol) (EVAL) leukodepletion filter membranes. The successful immobilization of different extents of BSA on EVAL membranes was endorsed by the Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectrum, variations in the wettability, electron microscopic images revealing the alterations in the fiber diameter and porousness of membranes. An interesting backlash was encountered in the in vitro plasma protein adsorption and in vitro platelet adhesion on the BSA-immobilized membranes as they exhibited a higher protein adsorption and lower platelet adhesion in comparison with neat EVAL. The infiltration of immobilized BSA to the leukodepletion capacity of those membranes was preliminarily studied by in vitro blood-material interactions followed by typical whole blood filtration of selected membranes. The data presented highlight that BSA moieties do not particularly affect the white blood cell (WBC) adhesion and platelet adhesion of EVAL membranes whereas resulted in a significantly higher red blood cell (RBC) recovery and decreased hemolysis under in vitro conditions. Accordingly, BSA-immobilized EVAL membranes were made to asymmetric leukodepletion filters; however, their further evaluation by whole blood filtration ensued that immobilization of BSA could not particularly enhance the overall whole blood leukodepletion efficacy of EVAL membranes.

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“…Cotton wool fibers were first used as the filter material to remove leucocytes from blood [6]. Subsequently, different synthetic material fibers were developed for the leucocyte filtration technique, which includes cellulose acetate [7], polyethylene terephthalate (PET) [5], polyurethane [8], polybutylene terephthalate [9,10], polypropylene (PP) [11], polyethylene vinyl alcohol (EVAL) [12], etc. In addition, recent studies of leucocyte depletion techniques revealed that many physical and chemical methods were used for modifying material surface characteristics.…”

Section: Introductionmentioning

confidence: 99%

Application of 4T-PET fibers/nonwovens for leucocyte filters

Huang

Lin

et al. 2018

Journal of Industrial Textiles

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Polyethylene terephthalate (PET) fiber with four T-lobe cross section (4T-fiber) has been applied to textile products due to the structural characteristic of micro-grooved channels to provide the high wicking, warming, and elastic properties. In this study, nonwoven (NW) filters composed of one layer of 4T-PET NW (containing 65% 4T-fibers) with one, three, or five layers of polypropylene (PP) NW (4T-PET NW + PP NW × 1, 3, or 5) were proposed for blood filtration to remove leucocytes to reduce blood transfusion-related adverse reactions. The removal rate of leucocytes could be enhanced when the PP NW layers were increased. The presence of the 4T-PET NW on the surface of five layers of PP NW (4T-PET NW + PP NW × 5) filters resulted in a higher erythrocyte recovery rate without losing the capability to remove leucocytes and platelets. Based on the theoretical analysis, leucodepletion filters were further prepared by stacking four 4T-PET NW + PP NW × 5 units together. The optimum filtration results of the filters for filtrating 110 mL of red blood cell concentrates could be prepared when four units of 4T-PET NW + PP × 5 NW placed in a series combination.

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A novel leukodepletion filter from electrospun poly(ethylene-vinyl alcohol) membranes and evaluation of its efficiency

Cited by 8 publications

References 41 publications

Sulfobetaine‐functionalized electrospun poly(ethylene‐co‐vinyl alcohol) membranes for blood filtration

Sulfobetaine‐functionalized electrospun poly(ethylene‐co‐vinyl alcohol) membranes for blood filtration

Investigation of the potency of leukodepletion filter membranes immobilized with bovine serum albumin via polydopamine spacer

Application of 4T-PET fibers/nonwovens for leucocyte filters

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