A novel lipase-based stationary phase in liquid chromatography

Enantioselective Nano Liquid Chromatographic Separation of Racemic Pharmaceuticals: A Facile One‐Pot In Situ Preparation of Lipase‐Based Polymer Monoliths in Capillary Format

“…However, with the aid of new technology such as protein nuclear magnetic resonance (NMR), Xray crystallography, and docking studies, the controversial mechanisms are being revealed and the binding modes of a number of protein-guest complexes have become known for many proteins. 15 For example, lipases, a class of protein that has been extensively used in kinetic resolution of racemates, 16 have been previously immobilized on epoxy silica and aminopropyl silica support [17][18][19] in conventional stainless steel columns (ID 2-4.8 mm) for chiral separations. 15 For example, lipases, a class of protein that has been extensively used in kinetic resolution of racemates, 16 have been previously immobilized on epoxy silica and aminopropyl silica support [17][18][19] in conventional stainless steel columns (ID 2-4.8 mm) for chiral separations.…”

“…CALB-based CSPs have been reported via immobilization onto macroporous silica gel 18,19 ; it has also been immobilized on azlactone photografted polymer monolith for biodesel production. CALB-based CSPs have been reported via immobilization onto macroporous silica gel 18,19 ; it has also been immobilized on azlactone photografted polymer monolith for biodesel production.…”

“…[13][14][15] Albeit protein phases are expensive, delicate in handling, and their performance is low compared to other CSPs, they impose excellent enantioselectivities and broad substrate applicability. 15 For example, lipases, a class of protein that has been extensively used in kinetic resolution of racemates, 16 have been previously immobilized on epoxy silica and aminopropyl silica support [17][18][19] in conventional stainless steel columns (ID 2-4.8 mm) for chiral separations. To the best of our knowledge, lipase has not been immobilized on monolithic support in situ fused glass capillary (ID 150 μm) for the purpose of chiral separations.…”

“…Candida antarctica lipase B (CALB) is one of the widely used lipases owing to its high degree of selectivity and broad range of industrial applications. CALB-based CSPs have been reported via immobilization onto macroporous silica gel 18,19 ; it has also been immobilized on azlactone photografted polymer monolith for biodesel production. 20 Despite the huge amount of research on lipases immobilization, little attention has been paid to their immobilization on polymer monoliths for the purpose of chiral separation.…”

Lipase as a Chiral Selector Immobilised on Carboxylated Single-Walled Carbon Nanotubes and Encapsulated in the Organic Polymer Monolithic Capillary for Nano-High Performance Liquid Chromatography Enantioseparation of Racemic Pharmaceuticals

“…Lipases have also been previously used for the chiral separation of enantiomers [ 42 , 43 , 44 , 45 ]. Being chiral in nature, lipases can interact with chiral analytes to form solids, with specific interactions leading to the successful resolution of enantiomers [ 46 ].…”