A Novel Medium for Enhancing Callus Growth of Hazel (Corylus avellana L.)

Abstract

Paclitaxel is a powerful antimitotic agent with excellent activity against a range of cancers. Hazel has been described as a paclitaxel-producing species among angiosperms. Fast-growing callus is a prerequisite for the success of callus production and then paclitaxel production. Therefore, optimizing the medium culture for enhancing callus growth is a crucial step for paclitaxel production. In this research, Murashige and Skoog (1962) (MS) medium was optimized for improving callus growth of hazel (Corylus avellana L.). The M10 medium (MS medium with pH 6.0 and supplemented with 1000 mg l−1 spirulina powder, 1000 mg l−1 casein hydrolysate and 3 g l−1 gelrite) significantly improved hazel callus growth. This modified MS medium increased callus fresh weight (55.8%) as compared to the control. M10 medium increased fatty acids yield of callus (66.7%) as compared to the control. Liquid M10 medium maintained growth over a longer period of time and also increased slightly, the paclitaxel production as compared to the control. This novel medium is promising for facilitating the mass production of hazel callus as a source of valuable metabolites including paclitaxel, linoleic and oleic acids.

Elicitors Derived from Hazel (Corylus avellana L.) Cell Suspension Culture Enhance Growth and Paclitaxel Production of Epicoccum nigrum

“…The callus of hazel ( C. avellana ) was obtained from seed cotyledons on MS medium supplemented with 0.2 mg/l 6-benzylaminopurine and 2 mg/l 2,4-dichlorophenoxyacetic acid and solidified with 8 g/l agar agar 50 . The C. avellana CSC was established with transferring 5 g callus into 250 ml flasks containing 100 ml medium and were maintained at 25 °C in darkness on gyratory shakers at 110 rpm.…”

Revestimento biodegradável à base de Spirulina platensis na conservação pós-colheita de goiaba Paluma mantidas sob diferentes temperaturas de armazenamento

“…Por esse motivo vem sendo intensamente estudada em novas linhas de pesquisas, como o desenvolvimento de biofilmes estruturais, para o recobrimento de frutos na fase de pós-colheita. Muitos estudos têm focado na biofertilização e bioestimulação de plantas na fase de cultivo (Godlewska et al, 2016;Michalak et al, 2017;Salehi et al, 2017).…”

Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

“…Callus of C. avellana (ecotype Gerd Ashkorat) was produced from seed cotyledons on MS medium 60 supplemented with 2 mg l −1 2, 4-D and 0.2 mg l −1 BAP, and 8 g l −1 agar agar 6 . CSC of C. avellana was obtained as described by Salehi et al 6,7,10,12 . (2020) 10:5427 | https://doi.org/10.1038/s41598-020-62196-4 www.nature.com/scientificreports www.nature.com/scientificreports/ elicitor preparation.…”

“…Control received an equal volume of water (for CE)/ PDB (for CF)/ water including 1% (v/v) acetic acid (for CW and chitin). The growth curve of C. avellana cells has been given elsewhere 6 . cell growth measurement.…”

“…1). Extracellular and intracellular paclitaxel were extracted from the cells and culture medium using a procedure described by Salehi et al 6,7,12 . Filtering all samples was performed by 0.22 µm cellulose acetate syringe filters before HPLC analysis.…”