A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine

Iio, Kaori; Nakauchi, Mina; Yamagami, Shigemasa; Tsutsumi, Makiko; Hori, Hiroshi; Naruse, Kiyoshi; Mikami, Hiroshi; Shima, Akihiro; Suzuki, Norio

doi:10.1016/j.cbpc.2004.12.006

Cited by 11 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, GC-C1 and GC-C2 are two GC-C subtypes so far characterized in the European (Anguilla anguilla) and Japanese eels (11,39,69). Two subtypes, OlGC6 and OlGC9, are also expressed in medaka (Oryzias latipes) (36), while only one GC-C type has been found in mammals and it is the predominant GC in the Values are means Ϯ SE; n ϭ 5-6 for the responses of each anterior and posterior intestinal segment to GN, UGN, and RGN, respectively. Peptides were applied to the mucosal half-chamber (2-ml volume) of an Ussing chamber in a cumulative manner.…”

Section: Tissue-specific Responses To Eel Gn Ugn and Rgnmentioning

confidence: 99%

“…intestine (4). Nonetheless, stimulation of fish and mammalian GC-Cs expressed in cell culture lines by GN or UGN leads to significant guanylyl cyclase activity and increases in cGMP (10,13,15,36,53,69), yet, in the anterior intestine of marine teleosts, these increases may not be occurring in significant amounts (if at all) to produce the downstream effects observed in the posterior intestine.…”

Section: Tissue-specific Responses To Eel Gn Ugn and Rgnmentioning

confidence: 99%

See 1 more Smart Citation

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Ruhr

Bodinier

Mager

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

The physiological effects of guanylin (GN) and uroguanylin (UGN) on fluid and electrolyte transport in the teleost fish intestine have yet to be thoroughly investigated. In the present study, the effects of GN, UGN, and renoguanylin (RGN; a GN and UGN homolog) on short-circuit current ( Isc) and the transport of Cl−, Na+, bicarbonate (HCO3−), and fluid in the Gulf toadfish ( Opsanus beta) intestine were determined using Ussing chambers, pH-stat titration, and intestinal sac experiments. GN, UGN, and RGN reversed the Isc of the posterior intestine (absorptive-to-secretory), but not of the anterior intestine. RGN decreased baseline HCO3− secretion, but increased Cl− and fluid secretion in the posterior intestine. The secretory response of the posterior intestine coincides with the presence of basolateral NKCC1 and apical cystic fibrosis transmembrane conductance regulator (CFTR), the latter of which is lacking in the anterior intestine and is not permeable to HCO3− in the posterior intestine. However, the response to RGN by the posterior intestine is counterintuitive given the known role of the marine teleost intestine as a salt- and water-absorbing organ. These data demonstrate that marine teleosts possess a tissue-specific secretory response, apparently associated with seawater adaptation, the exact role of which remains to be determined.

show abstract

Section: Tissue-specific Responses To Eel Gn Ugn and Rgnmentioning

confidence: 99%

Section: Tissue-specific Responses To Eel Gn Ugn and Rgnmentioning

confidence: 99%

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Ruhr

Bodinier

Mager

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Functional expression studies showed that exposure of cells expressing these GC-C-like proteins to the three eel guanylins induced dosedependent increases in cGMP accumulation with different potencies. At least two cDNAs encoding GC-C receptors are cloned in medaka (Mantoku et al, '99;Iio et al, 2005) and their orthologs can be identified in the genome database of pufferfish. Thus, the guanylin system may have diversified into multiple ligands and receptors in teleost fishes, particularly in the eel that has extraordinary osmoregulatory ability.…”

Section: Guanylin Familymentioning

confidence: 99%

Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones

et al. 2006

View full text Add to dashboard Cite

Fish endocrinologists are commonly motivated to pursue their research driven by their own interests in these aquatic animals. However, the data obtained in fish studies not only satisfy their own interests but often contribute more generally to the studies of other vertebrates, including mammals. The life of fishes is characterized by the aquatic habitat, which demands many physiological adjustments distinct from the terrestrial life. Among them, body fluid regulation is of particular importance as the body fluids are exposed to media of varying salinities only across the thin respiratory epithelia of the gills. Endocrine systems play pivotal roles in the homeostatic control of body fluid balance. Judging from the habitat-dependent control mechanisms, some osmoregulatory hormones of fish should have undergone functional and molecular evolution during the ecological transition to the terrestrial life. In fact, water-regulating hormones such as vasopressin are essential for survival on the land, whereas ion-regulating hormones such as natriuretic peptides, guanylins and adrenomedullins are diversified and exhibit more critical functions in aquatic species. In this short review, we introduce some examples illustrating how comparative fish studies contribute to general endocrinology by taking advantage of such differences between fishes and tetrapods. In a functional context, fish studies often afford a deeper understanding of the essential actions of a hormone across vertebrate taxa. Using the natriuretic peptide family as an example, we suggest that more functional studies on fishes will bring similar rewards of understanding. At the molecular level, recent establishment of genome databases in fishes and mammals brings clues to the evolutionary history of hormone molecules via a comparative genomic approach. Because of the functional and molecular diversification of ion-regulating hormones in fishes, this approach sometimes leads to the discovery of new hormones in tetrapods as exemplified by adrenomedullin 2.

show abstract

“…In addition to the two medaka fish homologues (OlGC2 and OlGC7) of mammalian GC-A (Yamagami et al, 2001(Yamagami et al, , 2003 and one medaka fish homologue (OlGC1) of mammalian GC-B (Takeda and Suzuki, 1999), we have isolated another full-length cDNA clone encoding a medaka fish homologue of mammalian STa/guanylin receptor/GC-C and designated it OlGC9 (Iio et al, 2005). Therefore, medaka fish has two different genes, OlGC6 and OlGC9, whose translating products belong to the STa/guanylin receptor/GC-C subfamily.…”

Section: How Many Gc Members Does Medaka Fish Have?mentioning

confidence: 99%

“…Since then, it has been demonstrated that the α 1 and β 1 subunit genes are aligned in a tandem fashion separating by only a 1 kbp spacer sequence on the medaka fish genome (Mikami et al, 1999). OlGC6 and OlGC9, medaka fish homologues of mammalian -C (Mantoku et al, 1999;Iio et al, 2005), and OlGC1, a medaka fish homologue of mammalian GC-B, have been identified, and their complete nucleotide sequences of genomic DNAs have been determined (Mantoku et al, 1999;Takeda and Suzuki, 1999).…”

Section: Introductionmentioning

confidence: 99%

Diverse Forms of Guanylyl Cyclases in Medaka Fish – Their Genomic Structure and Phylogenetic Relationships to those in Vertebrates and Invertebrates

Yamagami

Suzuki

2005

Zoological Science

Self Cite

View full text Add to dashboard Cite

-Fish species such as medaka fish, fugu, and zebrafish contain more guanylyl cyclases (GCs) than do mammals. These GCs can be divided into two types: soluble GCs and membrane GCs. The latter are further divided into four subfamilies: (i) natriuretic peptide receptors, (ii) STa/guanylin receptors, (iii) sensory-organ-specific membrane GCs, and (iv) orphan receptors. Phylogenetic analyses of medaka fish GCs, along with those of fugu and zebrafish, suggest that medaka fish is a much closer relative to fugu than to zebrafish. Analyses of nucleotide data available on a web site (http://www.ncbi. nlm.nih.gov/) of GCs from a range of organisms from bacteria to vertebrates suggest that gene duplication, and possibly chromosomal duplication, play important roles in the divergence of GCs. In particular, the membrane GC genes were generated by chromosomal duplication before the divergence of tetrapods and teleosts.

show abstract

A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine

Abstract: -A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone

Cited by 11 publications

References 45 publications

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Contribution of comparative fish studies to general endocrinology: structure and function of some osmoregulatory hormones

Diverse Forms of Guanylyl Cyclases in Medaka Fish – Their Genomic Structure and Phylogenetic Relationships to those in Vertebrates and Invertebrates

Contact Info

Product

Resources

About