A novel metal–organic framework loaded with abundant N-(aminobutyl)-N-(ethylisoluminol) as a high-efficiency electrochemiluminescence indicator for sensitive detection of mucin1 on cancer cells

Jiang, Xinya; Wang, Zili; Wang, Haijun; Zhuo, Ying; Yuan, Ruo; Chai, Yaqin

doi:10.1039/c7cc05495e

Cited by 89 publications

(50 citation statements)

References 34 publications

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“…[4] However, the low stability of H 2 O 2 at room temperature hampers the successful application of the luminol-H 2 O 2 ECL system. [5] Therefore, the search for durable co-reactants as alternatives to H 2 O 2 has been investigated.…”

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confidence: 99%

Single‐Atom Iron Boosts Electrochemiluminescence

et al. 2020

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The traditional luminol–H2O2 electrochemiluminescence (ECL) sensing platform suffers from self‐decomposition of H2O2 at room temperature, hampering its application for quantitative analysis. In this work, for the first time we employ iron single‐atom catalysts (Fe‐N‐C SACs) as an advanced co‐reactant accelerator to directly reduce the dissolved oxygen (O2) to reactive oxygen species (ROS). Owing to the unique electronic structure and catalytic activity of Fe‐N‐C SACs, large amounts of ROS are efficiently produced, which then react with the luminol anion radical and significantly amplify the luminol ECL emission. Under the optimum conditions, a Fe‐N‐C SACs–luminol ECL sensor for antioxidant capacity measurement was developed with a good linear range from 0.8 μm to 1.0 mm of Trolox.

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confidence: 99%

Single‐Atom Iron Boosts Electrochemiluminescence

et al. 2020

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“…It has been shown that ABEI can be easily incorporated in the skeleton of porous conducting polymer hydrogels, providing a short diffusion distance between the luminophore and the hydrogen peroxide coreactant, thereby realizing a sensitive in situ monitoring of the oxygen radicals released from cells [33]. It has also found application as a highly efficient ECL signal probe for cancer cell analysis [34].…”

Section: Luminol-based Systemsmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

et al. 2020

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Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devices for ECL cell assays is introduced. To conclude, the direction of research in this field and its application prospects are described.

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“…However, the adopted synthesis involves multiple steps, desires tedious reactions without scalability. Moreover, the co-reactant like H 2 O 2 is also not quite stable at room temperature which also suppresses the efficiency of the ECL signal of luminol 16 . One can overcome this problem by generating the reactive oxygen species (ROS) by using co-reactant accelerators which can produce more ROS through the reduction of dissolved oxygen and enhances the stability of the ECL signal 17 .…”

Section: Introductionmentioning

confidence: 99%

Co-reactant-free self-enhanced solid-state electrochemiluminescence platform based on polyluminol-gold nanocomposite for signal-on detection of mercury ion

Raju

2021

Sci Rep

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Development of a self-enhanced solid-state ECL platform creates a straightforward experimental design for the fabrication of point-of-care applications. Herein, we develop a promising method for self-enhanced solid-state ECL platform of polyluminol gold nanocomposite on glassy carbon electrode [(PL-Au)nano/GCE] via simple one-step electrochemical deposition process without involving any additional co-reactants. The presence of gold nanoparticles (AuNPs) augments the electron transfer kinetics of PL (polyluminol) and enhances the solid-state ECL intensity and promotes label-free, excellent sensitivity, and selectivity to detect Hg2+ in physiological pH through signal-on mode. Unlike pristine PL/GCE, electrochemically co-deposited AuNPs in the (PL-Au)nano/GCE composite, enable the co-reactant accelerator by improving the catalytic activity of PL towards oxygen reduction reaction (ORR) yielding in-situ ROS (co-reactant) generation. Further, the ECL intensity of (PL-Au)nano/GCE composite, gradually increases with each addition of Hg2+ ion. This is because of the formation of an amalgamation of Au-Hg on (PL-Au)nano/GCE composite surface which further accelerates the yield of in-situ ROS and enhances the intensity of ECL. Whereas no ECL signals changes were observed for PL/GCE composite. The proposed self-enhanced solid-state ECL platform is selectively sensing the Hg2+ ion in the linear range of 0.3–200 nM with a detection limit of 0.1 nM. The demonstrated (PL-Au)nano/GCE platform might pave new avenues for further studies in the solid-state ECL platform which could be more useful in on-site monitoring of clinical bioassay and immunosensors.

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A novel metal–organic framework loaded with abundant N-(aminobutyl)-N-(ethylisoluminol) as a high-efficiency electrochemiluminescence indicator for sensitive detection of mucin1 on cancer cells

Cited by 89 publications

References 34 publications

Single‐Atom Iron Boosts Electrochemiluminescence

Single‐Atom Iron Boosts Electrochemiluminescence

Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis

Co-reactant-free self-enhanced solid-state electrochemiluminescence platform based on polyluminol-gold nanocomposite for signal-on detection of mercury ion

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