A novel method for purification of recombinant adenoassociated virus vectors on a large scale

Wu, Xiaobing; Dong, Xiaoyan; Wu, Zhijian; Cao, Hui; Niu, Dong-Bin; Jian-guo, QU; Wang, Hong; Hou, Yong

doi:10.1007/bf03187263

Cited by 54 publications

(53 citation statements)

References 7 publications

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“…The pSNAV1/GDF11 was transfected into 293 T-cells and large-scale rAAV production and purification were described previously. 42 Viral preparations used for animal transduction had titers between 1 × 10 12 and 1 × 10 13 viral genome particles per 1 ml.…”

Section: Methodsmentioning

confidence: 99%

GDF11 Protects against Endothelial Injury and Reduces Atherosclerotic Lesion Formation in Apolipoprotein E-Null Mice

et al. 2016

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Section: Methodsmentioning

confidence: 99%

GDF11 Protects against Endothelial Injury and Reduces Atherosclerotic Lesion Formation in Apolipoprotein E-Null Mice

et al. 2016

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“…Three days later, cultures including transfected HEK 293 cells and medium were collected. Ten percent (v/v) chloroform was added to the collected cultures, and vigorously shaken for 1 h [14] . Then solid NaCl was added until the final concentration was 1 mol/L by shaking at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Adeno-associated virus vectors simultaneously encoding VEGF and angiopoietin-1 enhances neovascularization in ischemic rabbit hind-limbs

Chen

Tan

Dong

et al. 2007

Acta Pharmacologica Sinica

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“…rAAV/GFP or rAAV/HO-1 was generated in BHK-21 cells (American Type Culture Collection, Manassas, VA) by transfection of vector plasmid and subsequent rescue rAAVs (serotype 2) by co-infection with recombinant HSV1-rc/∆UL2, which is essential for viron packaging. A large scale of rAAV was purified as described [12] .…”

Section: Methodsmentioning

confidence: 99%

Adeno-associated virus-mediated heme oxygenase-1 gene transfer suppresses the progression of micronodular cirrhosis in rats

Tsui¹,

Schlitt²

2006

Z Gastroenterol

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AIM:To test the hypothesis that enhancement of the activity of heme oxygenase can interfere with processes of fibrogenesis associated with recurrent liver injury, we investigated the therapeutic potential of over-expression of heme oxygense-1 in a CCl 4 -induced micronodular cirrhosis model. Methods:Recombinant adeno-associated viruses carrying rat HO-1 or GFP gene were generated. 1×10 12 vg of adeno-associated viruses were administered through portal injection at the time of the induction of liver fibrosis.Results: Conditioning the rat liver with over-expression of HO-1 by rAAV/HO-1 significantly increased the HO enzymatic activities in a stable manner. The development of micronodular cirrhosis was significantly inhibited in rAAV/HO-1-transduced animals as compared to controls. Portal hypertension was markedly diminished in rAAV/HO-1-transduced animals as compared to controls, whereas there are no significant changes in systolic blood pressure. This finding was accompanied with improved liver biochemistry, less infiltrating macrophages and less activated hepatic stellate cells (HSCs) in rAAV/ HO-1-transduced livers. ConClusIons

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A novel method for purification of recombinant adenoassociated virus vectors on a large scale

Cited by 54 publications

References 7 publications

GDF11 Protects against Endothelial Injury and Reduces Atherosclerotic Lesion Formation in Apolipoprotein E-Null Mice

GDF11 Protects against Endothelial Injury and Reduces Atherosclerotic Lesion Formation in Apolipoprotein E-Null Mice

Adeno-associated virus vectors simultaneously encoding VEGF and angiopoietin-1 enhances neovascularization in ischemic rabbit hind-limbs

Adeno-associated virus-mediated heme oxygenase-1 gene transfer suppresses the progression of micronodular cirrhosis in rats

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