A novel method to scale up fungal endophyte isolations

Greenfield, Melinda; Pareja, R; Ortiz, Viviana; Gómez-Jiménez, María I.; Vega, Fernando E.; Parsa, Soroush

doi:10.1080/09583157.2015.1033382

Cited by 32 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The root pieces were pre-washed in 0.05% Triton X-100 for three minutes and then surface-sterilized by immersing in 0.5% NaOCl (diluted in 0.05% Triton X-100) for three minutes, ethanol (70%) for one minute and then rinsing three times in sterile distilled water for 15 s each rinse. The bulk surface-sterilization system described by Greenfield et al (2015) was used in all surface-sterilizations. To confirm that surface-sterilization was effective, eight root pieces were randomly selected from each block (with each block containing 24 root pieces in total) to make imprints on 75% PDA (in 100 mm × 15 mm Petri dishes) by gently pressing the root piece onto the surface of the agar ( Schulz et al, 1998 ).…”

Section: Methodsmentioning

confidence: 99%

Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…A total of 582 seedlings met these conditions ( Table 1 ). These seedlings were surface-sterilized in bulk following protocols developed by our research team ( Greenfield et al. 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris

et al. 2016

Self Cite

View full text Add to dashboard Cite

We conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11 Colombian cultivars of the common bean (Phaseolus vulgaris). The survey yielded 394 endophytic isolates belonging to 42 taxa, as identified by sequence analysis of the ribosomal DNA internal transcribed spacer (ITS) region. Aureobasidium pullulans was the dominant endophyte, isolated from 46.7 % of the samples. Also common were Fusarium oxysporum, Xylaria sp., and Cladosporium cladosporioides, but found in only 13.4 %, 11.7 %, and 7.6 % of seedlings, respectively. Endophytic colonization differed significantly among common bean cultivars and seedling parts, with the highest colonization occurring in the first true leaves of the seedlings.

show abstract

“…Sterilization using method of Greenfield et al (2015) which leaves soaked in the solution of 0.5 % NaOCl for 3 minutes, 70 % alcohol for 1 minute and rinsed with sterile distilled water for 3 times each 15 seconds and then dried using sterile wipes. Each leaf was cut 1 cm x 1 cm as much as 6 leaf pieces, so the Petri dish contained 6 leaf pieces.…”

Section: Sampling and Isolation Of Endophytic Fungimentioning

confidence: 99%

Distribution of the Endophytic Fungi in Apple Leaves

Afandhi¹,

Choliq²,

Anggrilika³

et al. 2018

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

Endophytic fungi have roles to protect the plant from herbivorous insects and diseases, and to support the absorption process of nutrients needed by plants for photosynthesis. Related to the important role of endophytic fungi, research was aimed to identify fungal endophytes associated with young, mature and old leaves on apple and to evaluate the effect of leaf ages on the abundance and diversity of endophytic fungi. The research was conducted in Biological Control Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya from May to September 2016. Apple leaves sampling was done diagonally, and taken from nine apple trees. Based on macroscopic and microscopic characteristics, 38 isolates were identified. In the old apple leaves, it obtained 17 isolates that consisted of 5 genera, mature leaves obtained 14 isolates consisted of 6 genera, and young leaves 7 isolates obtained consists of 2 genera. The mature leaves have highest abundance and diversity of endophytic fungi compared to young and old leaves. Endophytic fungi apple leaf dominated by Aspergillus. Mature and young leaves were different from old one based on Bray-Curtis similarity.

show abstract

A novel method to scale up fungal endophyte isolations

Cited by 32 publications

References 10 publications

Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris

Distribution of the Endophytic Fungi in Apple Leaves

Contact Info

Product

Resources

About