A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes

Devran, Zübeyir; Polat, İlknur; Mıstanoğlu, İbrahim; Baysal, Ömür

doi:10.1007/s13313-018-0570-z

Cited by 16 publications

(7 citation statements)

References 12 publications

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“…incognita was confirmed using species-specific primers. The primers used were MincF1 ( ) and MincR1 ( ) as forward and reverse primers, respectively [ 23 ]. The Cox-1 gene primer was used as a positive control [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Chemo-profiling of Purpureocillium lilacinum and Paecilomyces variotii isolates using GC-MS analysis, and evaluation of their metabolites against M. incognita

Patidar,

Prasad,

Sagar

et al. 2024

PLoS ONE

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Nematophagous fungi are the best alternatives to chemical nematicides for managing nematodes considering environmental health. In the current study, activity of metabolites from ten isolates of Purpureocillium lilacinum (Thom) Luangsa-ard (Hypocreales: Ophiocordycipitaceae) and two isolates of Paecilomyces variotii Bainier (Eurotiales: Trichocomaceae), were examined to inhibit the hatching of Meloidogyne incognita (Kofoid & White) Chitwood (Tylenchida: Heteroderidae) eggs. At 100%, 50%, and 25% concentrations, respectively, the culture filtrate of the isolate P. lilacinum 6887 prevented 97.55%, 90.52%, and 62.97% of egg hatching. Out of all the isolates, Pl 6887, Pl 6553, and Pl 2362 showed the greatest results in the hatching inhibition experiment.Gas chromatography-mass spectrometry (GC-MS) analysis revealed a variety of nematicidal compounds from different isolates. A total of seven nematicidal compounds, including four very potent nematicidal fatty acids were found in the isolate Pl 6553. Secondary metabolites of the same isolate possess the highest M. incognita juvenile mortality, i.e., 43.33% and 92% after 48 hrs of treatment at 100 and 200 ppm concentrations, respectively. Significant difference was observed in juvenile mortality percentage among the isolate having highest and lowest nematicidal compounds. Nematicidal fatty acids like myristic and lauric acid were found for the first time in P. lilacinum. Multiple vacuole-like droplets were found inside the unhatched eggs inoculated with the culture filtrate of isolate Pl 6887, and also in the juveniles that perished in the ethyl acetate extract of isolate Pl 6553.

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Section: Methodsmentioning

confidence: 99%

Chemo-profiling of Purpureocillium lilacinum and Paecilomyces variotii isolates using GC-MS analysis, and evaluation of their metabolites against M. incognita

Patidar,

Prasad,

Sagar

et al. 2024

PLoS ONE

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“…Los métodos incluyen el uso de RFLP (Xu, et al, 2004), RAPD (Amplificación aleatoria de ADN polimórfico) (Tigano et al, 2010), sondas de microsatélites y ADN satélite, PCR multiplex (Saeki et al, 2002), iniciadores específicos SCAR (Zijlstra et al, 2000;Tigano et al, 2010;Jorge, 2016;Mattos, 2017) y PCR en tiempo real (Toyota et al 2008;Agudelo et al, 2011;De Weerdt et al, 2011;Sapkota et al, 2016). Técnicas como PCR multiplex son muy útiles en la identificación de varias especies de forma conjunta, debido a que este tipo de procedimientos permiten amplificar varios segmentos de ADN, en una sola reacción por medio del uso de dos o más sets de iniciadores (Bolívar et al 2014), esta técnica ha sido ampliamente empleada para la identificación y detección de M. incognita, M. enterolobii, M. javanica y M. arenaria (Hu et al 2011;Devran et al 2018;Wolf et al 2013), usando ADN extraído de agallas provocadas por el nematodo en China y Turquía, así como en países del sudeste europeo, con la finalidad de desarrollar un método rápido de identificación. Este tipo de técnica ha sido utilizada en la identificación de Meloidogyne spp., y Pratylenchus spp., mediante el uso de iniciadores específicos, a partir de muestras de suelo donde existen la presencia de otros nematodos (Saeki et al 2003).…”

Section: Identificación Molecularunclassified

Identification methods for root-knot nematode Meloidogyne

Zambrano

Cedeño²

2022

Manglar

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Las especies del género Meloidogyne, son de distribución mundial y en general inducen la formación de agallas o nódulos en las raíces de una amplia variedad de plantas, debido a esto, es conocido como "nematodo agallador". Las plantas afectadas manifiestan pérdida de turgencia de las hojas, clorosis y marchitamiento, lo que reduce el rendimiento de los cultivos. En el Ecuador han sido registradas varias especies de Meloidogyne, en plantas silvestres y cultivadas. Para el diagnóstico, la identificación de este fitonemátodo inicialmente fue mediante el uso de caracteres morfológicos y posteriormente se incorporó el uso del análisis del patrón de isoenzimas en gel de poliacrilamida. Sin embargo, las técnicas moleculares, que incluyen el análisis de secuencias parciales o totales de uno o más genes de importancia taxonómica, han generado una revolución en la Taxonomía en general, por lo que actualmente es una de las estrategias con enorme potencial tanto para la Identificación como para el estudio genético de Meloidogyne spp., debido a su alta sensibilidad y confiabilidad del resultado. El surgimiento de modernas técnicas de identificación permitirá una correcta identificación de Meloidogyne spp., lo que contribuye al diseño de adecuadas estrategias de manejo de este nematodo fitopatógeno.

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“…PPNs were determined using species-specific primers (Table 2). The PCR reactions were carried out on the SimpliAmp™ (Applied Biosystems, San Francisco, CA, USA) using the reaction conditions specified in former studies for different plant parasitic nematode species (Waeyenberge et al, 2000;Zijlstra et al, 2000;Wishart et al, 2002;Al-Banna et al, 2004;Gleason et al, 2008;Troccoli et al, 2008;Yan et al, 2008;Devran et al, 2018). PCR outcomes were analyzed on a 1.5% agarose gel in 1X TAE and visualized with Xpert Green DNA Stain using the Gel iX Imager (Intas Science, Göttingen, Germany).…”

Section: Pcr Amplificationmentioning

confidence: 99%

Anason yetiştirilen alanlarda önemli bitki paraziti nematodlarının dağılımı ve tanımlanması

Mıstanoğlu

Uysal

Devran

2022

TED

Self Cite

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Anise, Pimpinella anisum L. (Apiales: Apiaceae) is an important medicinal aromatic plant and can be attacked by different pests and pathogens. Plant parasitic nematodes are important pests that can be confused with nutrient deficiency or symptoms of various diseases or pests. Therefore, rapid and accurate identification of these pests is essential for integrated nematode management and rotation. In 2021, a survey was conducted in Bolvadin District of Afyonkarahisar Province, which is one of the most important anise production areas of Türkiye. Forty-two soil samples were collected from the anise growing areas in the district and 16 species-specific primers were used for molecular identification of plant parasitic nematodes. In the samples, Meloidogyne hapla Chitwood, 1949 (Tylenchida: Heteroderidae), Pratylenchus neglectus (Rensch, 1924) Filipjev & Schuurmans Stekhoven, 1941, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) and Aphelenchoides besseyi Christie, 1942 (Aphelenchida: Aphelenchoididae), were detected at the rates of 57% (24), 52% (22), 36% (15) and 7% (3), respectively. Plant parasitic nematodes were found in both single and mixed populations. In addition, A. besseyi was found for the first time in anise growing areas.

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A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes

Cited by 16 publications

References 12 publications

Chemo-profiling of Purpureocillium lilacinum and Paecilomyces variotii isolates using GC-MS analysis, and evaluation of their metabolites against M. incognita

Chemo-profiling of Purpureocillium lilacinum and Paecilomyces variotii isolates using GC-MS analysis, and evaluation of their metabolites against M. incognita

Identification methods for root-knot nematode Meloidogyne

Anason yetiştirilen alanlarda önemli bitki paraziti nematodlarının dağılımı ve tanımlanması

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