A novel non-contact communication between human keratinocytes and T cells: Exosomes derived from keratinocytes support superantigen-induced proliferation of resting T cells

Cai, Xiaowei; Zhu, Rong; Ran, Lei; Li, Yi‐Qian; Huang, Ke; Peng, Jing; Wang, He; Zhou, Chunli; Wang, Ru-Peng

doi:10.3892/mmr.2017.7492

Cited by 28 publications

(26 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For quantification of stably expressed proteins in different cellular compartments, we used the keratinocyte cell line HaCaT which expresses beta actin as a cytoplasmic protein 23 , lamins a/b as a nuclear protein 24 , and MHC-1 as a membrane protein 25 . We then compared the signal from those proteins after using 2 different permeabilizing agents, Saponin and Triton X-100 at a concentration of 0.1%.…”

Section: Methodsmentioning

confidence: 99%

Using chemiluminescence imaging of cells (CLIC) for relative protein quantification

Fisher

Sørensen

Abu-Humaidan

2020

Sci Rep

View full text Add to dashboard Cite

Cell physiology and cellular responses to external stimuli are partly controlled through protein binding, localization, and expression level. Thus, quantification of these processes is pivotal in understanding cellular biology and disease pathophysiology. However, it can be methodologically challenging. Immunofluorescence is a powerful technique, yet quantification by this method can be hampered by auto-fluorescence. Here we describe a simple, sensitive and robust chemiluminescence-based immunoassay (chemiluminescence imaging of cells; CLIC) for relative quantification of proteins. We first employed this method to quantify complement activation in cultured mammalian cells, and to quantify membrane protein expression, shedding, binding and internalization. Moreover, through specific membrane permeabilization we were able to quantify both cytosolic and nuclear proteins, and their translocation. We validated the CLIC quantification method by performing parallel experiments with other quantification methods like ELISA, qPCR, and immunofluorescence microscopy. The workflow of the immunoassay was found to be advantageous in certain instances when compared to these quantification methods. Since the reagents used for CLIC are common to other immunoassays with no need for specialized equipment, and due to the good linearity, dynamic range and signal stability inherent to chemiluminescence, we suggest that this assay is suitable for both small scale and high throughput relative protein quantification studies in whole cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Using chemiluminescence imaging of cells (CLIC) for relative protein quantification

Fisher

Sørensen

Abu-Humaidan

2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Human keratinocytes are epithelial cells that can synthesize keratin, which can act as a barrier in the body and protect the human body. Promoting the proliferation of human keratinocytes and extending their lifespan have important clinical significance [156][157][158]. AOS (enzymatic degradation, concentration: 10 mg/mL) cleaved from alginic acid polysaccharides stimulates the proliferation of keratinocytes in the presence of the non-heparin-binding epidermal growth factor (EGF) [159].…”

Section: Promotion Of Cell Proliferationmentioning

confidence: 99%

Advances in Research on the Bioactivity of Alginate Oligosaccharides

et al. 2020

View full text Add to dashboard Cite

Alginate is a natural polysaccharide present in various marine brown seaweeds. Alginate oligosaccharide (AOS) is a degradation product of alginate, which has received increasing attention due to its low molecular weight and promising biological activity. The wide-ranging biological activity of AOS is closely related to the diversity of their structures. AOS with a specific structure and distinct applications can be obtained by different methods of alginate degradation. This review focuses on recent advances in the biological activity of alginate and its derivatives, including their anti-tumor, anti-oxidative, immunoregulatory, anti-inflammatory, neuroprotective, antibacterial, hypolipidemic, antihypertensive, and hypoglycemic properties, as well as the ability to suppress obesity and promote cell proliferation and regulate plant growth. We hope that this review will provide theoretical basis and inspiration for the high-value research developments and utilization of AOS-related products.2 of 26 the 4 C 1 conformation and are linked by β-1,4-glycosidic bond, while in pG, all G residues are in the 1 C 4 conformation and are linked by an α-1,4-glycosidic bond. These features are responsible for the differences in their higher-order structure. For example, pG exhibits an egg-box-like conformation and usually forms stiffer 2-fold screw helical chains when dissolved in water, while pM forms belt chains through intra-molecular hydrogen bonds. Due to these dissimilarities, pM and pG, as well as their derivatives, will exhibit different activities [12].As the most abundant marine biomass and low-cost material, alginate has been extensively used in the food and medical industries. The widespread utilization is also driven by its favorable chemical properties and versatile activities. However, the applications of alginate have been greatly limited due to its high molecular weight and low bioavailability. Therefore, the degradation of high molecular weight polysaccharides into low molecular weight poly-or oligosaccharides is considered of great significance for improving their bioavailability, increasing the body's absorption of drugs, and fully utilizing the efficacy of polysaccharides. Recently, the degradation products of alginate, i.e., alginate oligosaccharides (AOS), have attracted increasing attention due to their biological activities and excellent solubility in water [13]. AOS can be depolymerized by different degradation methods, including enzymatic degradation, acid hydrolysis, and oxidative degradation [14]. Alginate lyases have been isolated from a wide range of organisms, including algae, marine invertebrates, and marine and terrestrial microorganisms, which can degrade alginate into unsaturated oligosaccharides by β-elimination [15,16]. Moreover, due to differences in degradation patterns, G content (G/M ratio), molecular weight, and spatial conformation of degradation products, AOS possess a variety of biological activities. They have anti-tumor properties [17], counteract oxidation [8], regulate immune respo...

show abstract

“…For example, IL-17A-treated keratinocytes released EVs containing β-defensin 2 and chemoattractants such as CXCL1, CXCL3, CXCL5, and CXCL6 125 . In addition, Staphylococcus aureus ( S. aureus ) enterotoxin B-loaded HaCaT cells (a keratinocyte cell line) released EVs containing MHC molecules, which promoted CD4 + and CD8 + T cell proliferation in vitro 126 . Keratinocyte-EVs were also reported to carry a set of miRNAs that helped discriminate between EV subpopulations 127 .…”

Section: Characteristics and Functions Of Evs From Cells Associated Wmentioning

confidence: 99%

Extracellular vesicles in Inflammatory Skin Disorders: from Pathophysiology to Treatment

Shao¹,

Fang²,

Li³

et al. 2020

Theranostics

View full text Add to dashboard Cite

Extracellular vesicles (EVs), naturally secreted by almost all known cell types into extracellular space, can transfer their bioactive cargos of nucleic acids and proteins to recipient cells, mediating cell-cell communication. Thus, they participate in many pathogenic processes including immune regulation, cell proliferation and differentiation, cell death, angiogenesis, among others. Cumulative evidence has shown the important regulatory effects of EVs on the initiation and progression of inflammation, autoimmunity, and cancer. In dermatology, recent studies indicate that EVs play key immunomodulatory roles in inflammatory skin disorders, including psoriasis, atopic dermatitis, lichen planus, bullous pemphigoid, systemic lupus erythematosus, and wound healing. Importantly, EVs can be used as biomarkers of pathophysiological states and/or therapeutic agents, both as carriers of drugs or even as a drug by themselves. In this review, we will summarize current research advances of EVs from different cells and their implications in inflammatory skin disorders, and further discuss their future applications, updated techniques, and challenges in clinical translational medicine.

show abstract

A novel non-contact communication between human keratinocytes and T cells: Exosomes derived from keratinocytes support superantigen-induced proliferation of resting T cells

Cited by 28 publications

References 33 publications

Using chemiluminescence imaging of cells (CLIC) for relative protein quantification

Using chemiluminescence imaging of cells (CLIC) for relative protein quantification

Advances in Research on the Bioactivity of Alginate Oligosaccharides

Extracellular vesicles in Inflammatory Skin Disorders: from Pathophysiology to Treatment

Contact Info

Product

Resources

About