A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans

Avrova, Anna O.; Whisson, Stephen C.; Pritchard, Leighton; Venter, Eduard; Luca, Sergio De; Hein, Ingo; Birch, Paul R. J.

doi:10.1099/mic.0.2006/002220-0

Cited by 25 publications

(19 citation statements)

References 61 publications

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“…infestans suppression subtractive hybridisation (SSH) cDNA library . It was suggested that such transcripts are produced by the action of enhancers recruiting the Polymerase II machinery, which then is delivered to downstream promoters forming noncoding transcripts in the process (Ling et al 2005;Avrova et al 2007).…”

Section: Discussionmentioning

confidence: 99%

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

2012

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The oomycete Pythium oligandrum is a mycoparasitic biocontrol agent that is able to antagonise several plant pathogens, and can promote plant growth. In order to test the potential usefulness of P. oligandrum as a biocontrol agent against late blight disease caused by the oomycete Phytophthora infestans, we investigated the interaction between P. oligandrum and Ph. infestans using the green fluorescent protein (GFP) as a reporter gene. A CaCl(2) and polyethylene-glycol-based DNA transformation protocol was developed for P. oligandrum and transformants constitutively expressing GFP were produced. Up to 56 % of P. oligandrum transformants showed both antibiotic resistance and fluorescence. Mycoparasitic interactions, including coiling of P. oligandrum hyphae around Ph. infestans hyphae, were observed with fluorescent microscopy. To gain further insights into the nature of P. oligandrum mycoparasitism, we sequenced 2376 clones from cDNA libraries of P. oligandrum mycelium grown in vitro, or on heat-killed Ph. infestans mycelium as the sole nutrient source. 1219 consensus sequences were obtained including transcripts encoding glucanases, proteases, protease inhibitors, putative effectors and elicitors, which may play a role in mycoparasitism. This represents the first published expressed sequence tag (EST) resource for P. oligandrum and provides a platform for further molecular studies and comparative analysis in the Pythiales.

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Section: Discussionmentioning

confidence: 99%

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

2012

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“…This plant pathogen parasitizes a large number of host species, including S. melongena and C. annuum [13, 14]. Although fungi and angiosperm plants last shared a common evolutionary ancestor about 2.5 billion years, p C.a .D and p S.m .D elements are included in the same group with P. infestans retrotransposon (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Ty1-copia group retrotransposons and the evolution of retroelements in several angiosperm plants: evidence of horizontal transmission

Woodrow¹

2012

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The phylogenetic relationships among thirty-seven new Ty1-copia group retrotransposons in seven angiosperm plants were examined by reverse transcriptase and ribonuclease H sequence analysis. Distribution pattern of the retrotransposons of closely related plant species generally reflects a close phylogenetic relationship. In contrast, we found that several retrotransposon sequences from the same genome exhibited a high degree of divergence and had a relatively high degree of identity versus retrotransposon sequences from widely divergent species, including an ancestral phytopathogen fungus. This finding supports the hypothesis that the horizontal transmission from phytopatogen organism to the host flowering plants could have played a role in the evolutionary dynamics of Ty1-copia group retrotransposons.

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“…Pinc1-1 and Pinc1-5, two experimentally proved ncRNAs belonging to the gene family Pinc1 , are up-regulated during this oomycete infection and growth in planta . The Pinc1 ncRNA members have a predicted secondary structure that precludes the RNA being processed into miRNAs (Avrova et al 2007). Thus they most likely function as regulatory lncRNAs.…”

Section: Long Non-coding Rnas In Eukaryotic Genomesmentioning

confidence: 99%

Non-coding RNAs in the development and pathogenesis of eukaryotic microbes

Chacko

Lin

2013

Appl Microbiol Biotechnol

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RNA has long been regarded as the important intermediary in the central dogma of gene expression. Recently, the importance of RNAs in the regulation of gene expression became evident with the identification and characterization of non-protein coding transcripts named noncoding RNA (ncRNA). The ncRNAs, small and long, are ubiquitously present in all three domains of life and are being recognized for their important roles in genome defense and development. Some of the ncRNAs have been associated with diseases and therefore they offer diagnostic and therapeutic potential. In this mini-review, we have highlighted some recent research on the ncRNAs identified in eukaryotic microbes, with special emphasis on fungi that are pathogenic to humans or plants when possible. It is our contention that further elucidation and understanding of ncRNAs will advance our understanding of the development and pathogenesis of eukaryotic microbes and offer alternatives in the diagnosis and treatment of the diseases caused by these pathogens.

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A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans

Cited by 25 publications

References 61 publications

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

Ty1-copia group retrotransposons and the evolution of retroelements in several angiosperm plants: evidence of horizontal transmission

Non-coding RNAs in the development and pathogenesis of eukaryotic microbes

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