A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein

Lin, Ya-Wen; Yen, Pauline H.

doi:10.1261/rna.42206

Cited by 46 publications

(54 citation statements)

References 37 publications

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“…The cells were stained with the anti-USP26-M antibody 17 and an Alexa Fluor 488 conjugated goat anti-rabbit IgG secondary antibody (Invitrogen, Carlsbad, CA, USA) according to Lin and Yen. 19 The acrosome was labeled with lectin peanut agglutinin (PNA) conjugated with Alexa Fluor 594 (Invitrogen) or a mouse monoclonal anti-acrosin antibody (GeneTex, Inc., Irvine, CA, USA), and the nucleus was stained with Hoechst 33258 (Invitrogen). The cells were then mounted and photographed with a Zeiss LSM510 confocal microscope (Carl Zeiss Microscopy GmbH, Jena, Germany).…”

Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Lin¹,

Hsu²,

Yen³

2013

Asian J Androl

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Spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.

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Section: Immunostaining Of Tissue Sections and Dispersed Germ Cellsmentioning

confidence: 99%

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Lin¹,

Hsu²,

Yen³

2013

Asian J Androl

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“…Human DAZAP-1 is a nucleocytoplasmic shuttling protein (Lin & Yen 2006) and its X. laevis ortholog, PRRP, associates with mRNA localization elements in Vg1 and VegT mRNAs (Zhao et al 2001). However, it is unclear whether DAZAP-1 plays an active role in mRNA localization and its role on these mRNAs appears to be independent of xDAZL.…”

Section: Mechanism Of Dazl-mediated Translational Stimulationmentioning

confidence: 99%

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Brook¹,

Smith²,

Gray³

2009

Reproduction

111

114

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Gametogenesis is a highly complex process that requires the exquisite temporal, spatial and amplitudinal regulation of gene expression at multiple levels. Translational regulation is important in a wide variety of cell types but may be even more prevalent in germ cells, where periods of transcriptional quiescence necessitate the use of post-transcriptional mechanisms to effect changes in gene expression. Consistent with this, studies in multiple animal models have revealed an essential role for mRNA translation in the establishment and maintenance of reproductive competence. While studies in humans are less advanced, emerging evidence suggests that translational regulation plays a similarly important role in human germ cells and fertility. This review highlights specific mechanisms of translational regulation that play critical roles in oogenesis by activating subsets of mRNAs. These mRNAs are activated in a strictly determined temporal manner via elements located within their 3 0 UTR, which serve as binding sites for trans-acting factors. While we concentrate on oogenesis, these regulatory events also play important roles during spermatogenesis. In particular, we focus on the deleted in azoospermia-like (DAZL) family of proteins, recently implicated in the translational control of specific mRNAs in germ cells; their relationship with the general translation initiation factor poly(A)-binding protein (PABP) and the process of cytoplasmic mRNA polyadenylation.

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“…It is expressed must abundantly in the testis and to a lesser degree in other tissues (Tsui et al 2000;Dai et al 2001;Pan et al 2005). In somatic cells, DAZAP1 is present mainly in the nucleus, and its association with the hnRNP particles makes it a bona fide hnRNP protein (Lin and Yen 2006). In mouse testes, DAZAP1 shows a dynamic distribution during spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…A role of DAZAP1 in transcription is suggested by its specific exclusion from the transcriptionally inert XY body in the nuclei of pachytene spermatocytes, and the requirement of active transcription for its nuclear localization (Vera et al 2002;Lin and Yen 2006). Its ability to shuttle between the nucleus and the cytoplasm supports its role in mRNA transport (Lin and Yen 2006).…”

Section: Introductionmentioning

confidence: 99%

DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice

Hsu¹,

Chen²,

Lin³

et al. 2008

RNA

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DAZAP1 (Deleted in Azoospermia Associated Protein 1) is a ubiquitous hnRNP protein that is expressed most abundantly in the testis. Its ability to shuttle between the nucleus and the cytoplasm and its exclusion from the transcriptionally inactive XY body in pachytene spermatocytes implicate it in mRNA transcription and transport. We generated Dazap1 mutant alleles to study the role of DAZAP1 in mouse development. Most mice homozygous for the null allele as well as a hypomorphic Fn allele died soon after birth. The few Dazap1Fn/Fn mice that survived could nonetheless live for more than a year. They appeared and behaved normally but were much smaller in size compared to their wild-type and heterozygous littermates. Both male and female Dazap1Fn/Fn mice were sterile. Males had small testes, and the seminiferous tubules were atrophic with increased numbers of apoptotic cells. The tubules contained many germ cells, including pachytene spermatocytes with visible XY-bodies and diplotene spermatocytes, but no post-meiotic cells. FACS analyses confirmed the absence of haploid germ cells, indicating spermatogenesis arrested right before the meiotic division. Female Dazap1Fn/Fn mice had small ovaries that contained normalappearing follicles, yet their pregnancy produced no progeny due to failure in embryonic development. The phenotypes of Dazap1 mutant mice indicate that DAZAP1 is not only essential for spermatogenesis, but also required for the normal growth and development of mice.

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A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein

Cited by 46 publications

References 37 publications

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

Mouse sperm acquire a new structure on the apical hook during epididymal maturation

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice

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