A Novel Nucleus-Targeted Protein Is Expressed in Barley Leaves during Senescence and Pathogen Infection

Krupinska, Karin; Haußühl, Kirsten; Schäfer, Anke; Kooij, Tom A.W. van der; Leckband, Gunhild; Lörz, Horst; Falk, Jon

doi:10.1104/pp.008565

Cited by 54 publications

(71 citation statements)

References 44 publications

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“…In crop plants like barley showing the typical sequential type of leaf senescence during ontogeny, it has also been shown that photosynthesis-related genes such as psbA, rbcl or rbcS are clearly down-regulated with the onset of leaf senescence (Humbeck et al, 1996), and that specific senescence-associated genes (SAGs), e.g. HvFP1 (Barth et al, 2004) or HvS40 (Becker and Apel, 1993;Krupinska et al, 2002) are up-regulated.…”

Section: Introductionmentioning

confidence: 99%

Successive maturation and senescence of individual leaves during barley whole plant ontogeny reveals temporal and spatial regulation of photosynthetic function in conjunction with C and N metabolism

Wiedemuth¹,

Müller²,

Kahlau³

et al. 2005

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 99%

Successive maturation and senescence of individual leaves during barley whole plant ontogeny reveals temporal and spatial regulation of photosynthetic function in conjunction with C and N metabolism

Wiedemuth¹,

Müller²,

Kahlau³

et al. 2005

Journal of Plant Physiology

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“…One of the senescence marker genes of barley is the HvS40 gene (Becker and Apel 1993;Krupinska et al 2002). The HvS40 gene is expressed under different situations of senescence ranging from dark induced senescence of detached leaves up to age dependent senescence of barley flag leaves (Becker and Apel 1993;Humbeck et al 1996;KleberJanke and Krupinska 1997;Krupinska et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Nuclear targeted AtS40 modulates senescence associated gene expression in Arabidopsis thaliana during natural development and in darkness

Fischer-Kilbienski¹,

Miao²,

Roitsch

et al. 2010

Plant Mol Biol

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The Arabidopsis thaliana AtS40-3 gene belongs to a group of genes sharing the conserved DUF548 sequence motif with up to now unknown function. One member of this group, the barley HvS40, was shown before to play a role in regulation of leaf senescence. Similar as the barley gene, AtS40-3 is induced during senescence and is also regulated in response to dark treatment, ABA, salicylic acid and pathogen attack. By localization of the GUS fusion protein, the AtS40-3 gene was shown to encode a nucleus targeted protein. The s40-3a mutant with a T-DNA insertion in the promoter region of the gene was observed to have a staygreen phenotype. By quantitative real-time PCR analyses expression of the AtS40-3 gene in this mutant was observed to be constitutive and not induced during senescence. This coincided with WRKY53 gene expression in nonsenescent leaves and lowered expression levels of WRKY53 and SAG12 at later stages of development. While in the wildtype expression of AtS40-3 was activated by darkness, in the s40-3a mutant the expression of AtS40-3 stayed at a low level. This coincided with lower expression of the SEN1 and SAG12 genes. In another promoter mutant with a T-DNA insertion further upstream of the coding sequence the levels of AtS40-3 and SAG12 transcripts increased in parallel both in a natural light-dark regime and in darkness. The data on gene expression in promoter T-DNA insertion mutants of the s40-3 gene indicate that AtS40 regulates senescence either by modulation of WRKY53 or by activation of SAG12 independent of WRKY53.

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“…It is required for both expression of SA-regulated genes and associated disease resistance, although this pathway is independent of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEIN1 (Desveaux et al, 2004). The barley (Hordeum vulgare) WHIRLY1 protein binds to the promoter of the senescence-associated gene HvS40, which is induced in natural and stress-induced senescence (Krupinska et al, 2002(Krupinska et al, , 2014a.…”

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confidence: 99%

WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

et al. 2015

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WHIRLY1 is largely targeted to plastids, where it is a major constituent of the nucleoids. To explore WHIRLY1 functions in barley (Hordeum vulgare), RNA interference-knockdown lines (W1-1, W1-7, and W1-9) that have very low levels of HvWHIRLY1 transcripts were characterized in plants grown under optimal and stress conditions. The WHIRLY1-1 (W1-1), W1-7, and W1-9 plants were phenotypically similar to the wild type but produced fewer tillers and seeds. Photosynthesis rates were similar in all lines, but W1-1, W1-7, and W1-9 leaves had significantly more chlorophyll and less sucrose than the wild type. Transcripts encoding specific subsets of chloroplast-localized proteins, such as ribosomal proteins, subunits of the RNA polymerase, and thylakoid nicotinamide adenine dinucleotide (reduced) and cytochrome b 6 /f complexes, were much more abundant in the W1-7 leaves than the wild type. Although susceptibility of aphid (Myzus persicae) infestation was similar in all lines, the WHIRLY1-deficient plants showed altered responses to nitrogen deficiency, maintaining higher photosynthetic CO 2 assimilation rates than the wild type under limiting nitrogen. Although all lines showed globally similar low nitrogen-dependent changes in transcripts and metabolites, the increased abundance of FAR-RED IMPAIRED RESPONSE1-like transcripts in nitrogen-deficient W1-7 leaves infers that WHIRLY1 has a role in communication between plastid and nuclear genes encoding photosynthetic proteins during abiotic stress.

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A Novel Nucleus-Targeted Protein Is Expressed in Barley Leaves during Senescence and Pathogen Infection

Cited by 54 publications

References 44 publications

Successive maturation and senescence of individual leaves during barley whole plant ontogeny reveals temporal and spatial regulation of photosynthetic function in conjunction with C and N metabolism

Successive maturation and senescence of individual leaves during barley whole plant ontogeny reveals temporal and spatial regulation of photosynthetic function in conjunction with C and N metabolism

Nuclear targeted AtS40 modulates senescence associated gene expression in Arabidopsis thaliana during natural development and in darkness

WHIRLY1 Functions in the Control of Responses to Nitrogen Deficiency But Not Aphid Infestation in Barley

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