A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment

Mueller, Berit; Koch, Dietmar; Lutz, Rainer; Schlegel, K. A.; Treccani, Laura; Rezwan, Kurosch

doi:10.1016/j.msec.2014.05.017

Cited by 17 publications

(19 citation statements)

References 38 publications

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“…Hess suggests that it is possible to obtain HA/protein scaffolds with controllable properties, concluding that adding BSA can tailor the porosity and adding fibrinogen increases the material's strength. This illustrates how active proteins can be directly incorporated into a ceramic suspension by sample preparation . Together, experiments point to the use of albumin, either alone or in combination with traditional materials, as promising scaffolds in tissue engineering applications.…”

Section: Local Albumin Administrationmentioning

confidence: 91%

“…Another approach is the incorporation of albumin into ceramic scaffolds. Mueller suggests the incorporation of albumin into complex‐shaped HAP scaffolds as biodegradable bone substitutes or drug delivery platforms . These scaffolds can be used in non‐load bearing applications.…”

Section: Local Albumin Administrationmentioning

confidence: 99%

“…These scaffolds can be used in non‐load bearing applications. After 8 weeks of implantation, the hydroxyapatite (HA) scaffold with albumin in vivo had the highest bone formation and bone regeneration rates . Hess suggests that it is possible to obtain HA/protein scaffolds with controllable properties, concluding that adding BSA can tailor the porosity and adding fibrinogen increases the material's strength.…”

Section: Local Albumin Administrationmentioning

confidence: 99%

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Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

et al. 2016

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Albumin is a major plasma protein that has become ubiquitous in regenerative medicine research. As such, many studies have examined its structure and advantageous properties. However, a systematic and comprehensive understanding of albumin's role, capabilities and therapeutic potential still eludes the field. In the present work, we review how albumin is applied in tissue engineering, including cell culture and storage, in vitro fertilization and transplantation. Furthermore, we discuss how albumin's physiological role extends beyond a carrier for metal ions, fatty acids, pharmacons and growth factors. Albumin acts as a bacteriostatic coating that simultaneously promotes attachment and proliferation of eukaryotic cells. These properties with the combination of free radical scavenging, neutrophil activation and as a buffer molecule already make the albumin protein beneficial in healing processes supporting functional tissue remodeling. Nevertheless, recent data revealed that albumin can be synthesized by osteoblasts and its local concentration is raised after bone trauma. Interestingly, by increasing the local albumin concentration in vivo, faster bone healing is achieved, possibly because albumin recruits endogenous stem cells and promotes the growth of new bone. These data also suggest an active role of albumin, even though a specific receptor has not yet been identified. Together, this discussion sheds light on why the extravascular use of the albumin molecule is in the scope of scientific investigations and why it should be considered as a local therapeutic agent in regenerative medicine. © 2016 BioFactors, 43(3):315-330, 2017.

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Section: Local Albumin Administrationmentioning

confidence: 91%

Section: Local Albumin Administrationmentioning

confidence: 99%

Section: Local Albumin Administrationmentioning

confidence: 99%

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Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

et al. 2016

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“…However, the application of pure HA scaffolds in bone tissue engineering is limited by their hardness, fragility, and lack of fiexibility[ 4 , 5 ].The fragility makes it impossible for HA to be used in load-bearing bone repair and substitute, and the HA powders can easily migrate from the implant site which may result in inappropriate ossification. Compared to pure HA, polymeric nanocomposites exhibit improvements in properties such as modulus, strength, and stiffness for bone repair[ 6 ]. Inorganic–organic composite materials are increasingly important due to the extraordinary properties arising from the synergism between the properties of their components [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Ectopic Osteogenesis and Scaffold Biodegradation of Nano-Hydroxyapatite-Chitosan in a Rat Model

Dong

Cui

et al. 2015

PLoS ONE

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The bone-formation and scaffold-biodegradation processes have not been fully characterized. This study aimed to determine the osteogenic ability of nHA-CS osteo-induced bone marrow mesenchymal stem cell (BMSC) composites and to explore the relationship between bone formation and scaffold biodegradation. The nHA-CS osteo-induced BMSC composites (nHA-CS+cells group) and the nHA-CS scaffolds (nHA-CS group) were implanted into the femoral spatium intermusculare of SD rats. At 2, 4, 6, 8, and 12 weeks post-implantation, the rat femurs were scanned using computerized tomography (CT), and the CT values of the implants were measured and comparatively analyzed. The implants were then harvested and subjected to hematoxylin and eosin (HE) and Masson's trichrome staining, and the percentages of bone area, scaffold area and collagen area were compared between the two groups. The CT values of the implants were higher in the nHA-CS+cells group than the nHA-CS group at the same time points (P < 0.05). Histological analysis revealed that de novo bone and collagen formation in the pores of the scaffolds gradually increased from 2 weeks post-implantation in both groups and that the scaffold gradually degraded as bone formation proceeded. However, more de novo bone and collagen formation and scaffold degradation occurred in the nHA-CS+cells group than in the nHA-CS group at the same time points (P < 0.05). In conclusion, nHA-CS osteo-induced BMSC composites are promising bone tissue engineering substitutes, and osteo-induced BMSCs can significantly enhance the osteogenic ability and play an active role in the degradation of nHA-CS scaffolds on par with bone formation.

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“…Moreover, the addition of proteins has the ability to improve the mechanical strength of the ceramics. In these systems, the characteristics of the proteins (solubility) strongly affect the magnitude of the release achieved under static conditions [110].…”

Section: Biomimetic Ceramics As Drug Delivery Platformsmentioning

confidence: 99%

Drug-Loaded Biomimetic Ceramics for Tissue Engineering

Díaz‐Rodríguez

Sánchez

2018

Pharmaceutics

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The mimesis of biological systems has been demonstrated to be an adequate approach to obtain tissue engineering scaffolds able to promote cell attachment, proliferation, and differentiation abilities similar to those of autologous tissues. Bioceramics are commonly used for this purpose due to their similarities to the mineral component of hard tissues as bone. Furthermore, biomimetic scaffolds are frequently loaded with diverse therapeutic molecules to enhance their biological performance, leading to final products with advanced functionalities. In this review, we aim to describe the already developed bioceramic-based biomimetic systems for drug loading and local controlled release. We will discuss the mechanisms used for the inclusion of therapeutic molecules on the designed systems, paying special attention to the identification of critical parameters that modulate drug loading and release kinetics on these scaffolds.

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A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment

Cited by 17 publications

References 38 publications

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

Ectopic Osteogenesis and Scaffold Biodegradation of Nano-Hydroxyapatite-Chitosan in a Rat Model

Drug-Loaded Biomimetic Ceramics for Tissue Engineering

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