2018
DOI: 10.3390/antib7040037
|View full text |Cite
|
Sign up to set email alerts
|

A Novel Panel of Rabbit Monoclonal Antibodies and Their Diverse Applications Including Inhibition of Clostridium perfringens Epsilon Toxin Oligomerization

Abstract: The pore-forming epsilon toxin (ETX) produced by Clostridium perfringens is among the most lethal bacterial toxins known. Sensitive antibody-based reagents are needed to detect toxin, distinguish mechanisms of cell death, and prevent ETX toxicity. Using B-cell immuno-panning and cloning techniques, seven ETX-specific monoclonal antibodies were generated from immunized rabbits. ETX specificity and sensitivity were evaluated via western blot, ELISA, immunocytochemistry (ICC), and flow cytometry. ETX-neutralizing… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
17
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
6

Relationship

4
2

Authors

Journals

citations
Cited by 8 publications
(17 citation statements)
references
References 60 publications
0
17
0
Order By: Relevance
“…To determine the specificity of ETX cytotoxicity in vivo , ETX was pre-incubated with a neutralizing monoclonal antibody, JL008, against ETX before injection into fish expressing hMAL-GFP (n = 4) (Linden et al., 2018). Confocal image analysis of identical experimental parameters revealed dextran well confined within vessels, normal CCtA lumen diameter, and steady blood flow through hMAL-GFP vessels (Video S11).…”
Section: Resultsmentioning
confidence: 99%
“…To determine the specificity of ETX cytotoxicity in vivo , ETX was pre-incubated with a neutralizing monoclonal antibody, JL008, against ETX before injection into fish expressing hMAL-GFP (n = 4) (Linden et al., 2018). Confocal image analysis of identical experimental parameters revealed dextran well confined within vessels, normal CCtA lumen diameter, and steady blood flow through hMAL-GFP vessels (Video S11).…”
Section: Resultsmentioning
confidence: 99%
“…Recently, Fischer rat (FRT) thyroid cell line was identified as a novel model to study ETX action [156]. The importance of ETX oligomerization in mediating cytotoxicity was recently came in light when a novel set of rabbit monoclonal antibodies blocked this step which subsequently abolishes ETX endocytosis and cellular vacuoaltion [157].…”
Section: Pathogenesis and Pathogenicitymentioning
confidence: 99%
“…Contrary to our results however, was the observation that ETX treatment increased lysosome concentration and acidic activity in MDCK cells. Finally, accumulation of RAB7+ endosomes and vacuoles has also been observed in CHO cells expressing MAL as well [97]. This indicates that although different cell types may share some ETX-induced endocytic pathways, the ultimate fate of these endosomes may be cell specific.…”
Section: Discussionmentioning
confidence: 88%
“…Finally, as ETX oligomerization/pore formation has been postulated as being required for ETX-induced cytotoxicity, ETX-oligomerization in whole cell lysates from BEC was confirmed via western blot (Fig 10I and 10J). Whole cell lysates from CHO cells expressing MAL were used as positive controls for ETX oligomerization [97]. ETX oligomers of approximately 150 kDA were observed in BEC and appeared to be time-dependent.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation