A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Zhu, Jun; Guo, Jun; Zhao, Hu; Zhang, Xu Tong; Li, Yong; Zhong, Jie

doi:10.3389/fmicb.2021.653809

Cited by 14 publications

(15 citation statements)

References 49 publications

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“…Typical examples of hypovirulent mycovirus application include Cryphonectria hypovirus 1 (CHV1), which is used to control chestnut blight caused by Cryphonectria parasitica in Europe, and the Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), which is used to control stalk break caused by Sclerotiorum sclerotiorum [ 13 , 14 , 15 , 16 ]. In recent years, many hypovirulence-associated mycoviruses have been found in other plant pathogenic fungi, such as Botryosphaeria cinerea debilitation-related virus (BcDRV), Botryosphaeria dothidea Chrysovirus 1 (BdCV1), Rhizoctonia solani partitivirus 2 (RSPV2), Rhizoctonia solani endornavirus 1 (RsEV1) and Colletotrichum liriopes partitivirus 1 (ClPV1) [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Wen

Wan

Zhang

et al. 2021

Viruses

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A novel mycovirus named Fusarium oxysporum alternavirus 1(FoAV1) was identified as infecting Fusarium oxysporum strain BH19, which was isolated from a fusarium wilt diseased stem of Lilium brownii. The genome of FoAV1 contains four double-stranded RNA (dsRNA) segments (dsRNA1, dsRNA 2, dsRNA 3 and dsRNA 4, with lengths of 3.3, 2.6, 2.3 and 1.8 kbp, respectively). Additionally, dsRNA1 encodes RNA-dependent RNA polymerase (RdRp), and dsRNA2- dsRNA3- and dsRNA4-encoded hypothetical proteins (ORF2, ORF3 and ORF4), respectively. A homology BLAST search, along with multiple alignments based on RdRp, ORF2 and ORF3 sequences, identified FoAV1 as a novel member of the proposed family “Alternaviridae”. Evolutionary relation analyses indicated that FoAV1 may be related to alternaviruses, thus dividing the family “Alternaviridae” members into four clades. In addition, we determined that dsRNA4 was dispensable for replication and may be a satellite-like RNA of FoAV1—and could perhaps play a role in the evolution of alternaviruses. Our results provided evidence for potential genera establishment within the proposed family “Alternaviridae”. Additionally, FoAV1 exhibited biological control of Fusarium wilt. Our results also laid the foundations for the further study of mycoviruses within the family “Alternaviridae”, and provide a potential agent for the biocontrol of diseases caused by F. oxysporum.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Wen

Wan

Zhang

et al. 2021

Viruses

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“…In Botrytis cinerea , the causal agent of gray mold disease on more than 1400 plant species, including many economically important crops, the Botrytis cinerea partitivirus 2 (BcPV-2) was found in hypovirulent isolates in apple, cucumber, oilseed rape, strawberry, table grapes, tobacco, and tomato, suggesting that this mycovirus might be responsible of the hypovirulence [ 75 ]. The Colletotrichum liriopes partitivirus 1 (ClPV-1) could reduce the virulence and conidia production of Colletotrichum liriopes , a fungus causing leaf anthracnose in many plants [ 76 ]. In forestry, in addition to the mycovirus model CHV-1 affecting C. parasitica , several mycoviruses triggering hypovirulence have been described in fungal pathogens.…”

Section: Mycoviruses: a Natural Source Of Fungal Hypovirulencementioning

confidence: 99%

New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference

Bocos-Asenjo

Niño‐Sánchez

Ginésy

2022

IJMS

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RNA-based strategies for plant disease management offer an attractive alternative to agrochemicals that negatively impact human and ecosystem health and lead to pathogen resistance. There has been recent interest in using mycoviruses for fungal disease control after it was discovered that some cause hypovirulence in fungal pathogens, which refers to a decline in the ability of a pathogen to cause disease. Cryphonectria parasitica, the causal agent of chestnut blight, has set an ideal model of management through the release of hypovirulent strains. However, mycovirus-based management of plant diseases is still restricted by limited approaches to search for viruses causing hypovirulence and the lack of protocols allowing effective and systemic virus infection in pathogens. RNA interference (RNAi), the eukaryotic cell system that recognizes RNA sequences and specifically degrades them, represents a promising. RNA-based disease management method. The natural occurrence of cross-kingdom RNAi provides a basis for host-induced gene silencing, while the ability of most pathogens to uptake exogenous small RNAs enables the use of spray-induced gene silencing techniques. This review describes the mechanisms behind and the potential of two RNA-based strategies, mycoviruses and RNAi, for plant disease management. Successful applications are discussed, as well as the research gaps and limitations that remain to be addressed.

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“…For the fungi host, the members of the family Partitiviridae have been reported from phytopathogenic fungi ( Aspergillus fumigatus , Botrytis cinerea , Cytospora sacchari , Rhizoctonia solani , Ustilaginoidea virens , etc. ), oomycete ( Pythium nunn ), biological control fungi ( Trichoderma harzianum ), and even human pathogenic fungi ( Talaromyces marneffei ) [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In general, the mycoviruses in this family contain two dsRNA segments which have a 1.4 to 2.4 kb genome.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Song,

Zhong,

Guan

et al. 2024

JoF

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Root rot as a result of Salvia miltiorrhiza is a common root disease caused by Fusarium spp., which has become one of the main diseases affecting the production of S. miltiorrhiza. Currently, several hypovirulence-related mycoviruses have been identified in many phytopathogenic fungi, including Fusarium spp., which show potential as biological controls. In this study, we report a new mycovirus, Fusarium oxysporum partitivirus 1 (FoPV1), isolated from F. oxysporum strain FCR51, which is a causal agent of S. miltiorrhiza dry rot. The FoPV1 genome contains two double-stranded RNA segments (dsRNA1 and dsRNA2). The size of dsRNA1 is 1773 bp, and it encodes a putative RNA-dependent RNA polymerase (RdRp). The dsRNA2 is 1570 bp in length, encoding a putative capsid protein (CP). Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of the RdRp and the CP proteins indicated that FoPV1 appears to be a new member of the family Partitiviridae that is related to members of the genus Gammapartitivirus. Pathogenicity assay showed that FoPV1 confers hypervirulence to its host, F. oxysporum. This is the first report of a partitivirus infecting F. oxysporum and the first hypovirulence-related mycovirus from the causal agent of S. miltiorrhiza dry rot.

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A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Cited by 14 publications

References 49 publications

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference

Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

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