A novel patches-selection method for the classification of point-of-care biosensing lateral flow assays with cardiac biomarkers

Fairooz, Towfeeq; McNamee, Sara; Finlay, Dewar; Ng, Kok Yew; McLaughlin, James

doi:10.1016/j.bios.2022.115016

Cited by 9 publications

(1 citation statement)

References 12 publications

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“…For visual and rapid analysis, AuNPs‐LFB diagnostic platform was applied in the present study. AuNPs‐LFB is a paper‐based biosensor that is a convenient POC testing technique on account of its high sensitivity and specificity, rapid detection, ease of use, low cost, and no need for trained personnel 29,30 . AuNPs‐LFB strips have been extensively used to diagnose various analytes, such as infectious pathogens, cancer biomarkers, and foodborne microbes 18 .…”

Section: Discussionmentioning

confidence: 99%

Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Chen,

Dong,

Shi

et al. 2024

Journal of Medical Virology

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Hepatitis C remains a global health problem, especially in poverty‐stricken areas. A rapid and sensitive point‐of‐care (POC) diagnostic tool is critical for the early detection and timely treatment of hepatitis C virus (HCV) infection. Here, for the first time, we reported a novel molecular diagnostic assay, termed reverse transcription multiple cross displacement amplification integrated with a gold‐nanoparticle‐based lateral flow biosensor (RT‐MCDA‐AuNPs‐LFB), which was developed for rapid, sensitive, specific, and visual identification of HCV. HCV‐RT‐MCDA induced rapid isothermal amplification through a specific primer set targeting the 5′untranslated region gene from the major HCV genotypes 1b, 2a, 3b, 6a, and 3a that are prevalent in China. The optimal reaction temperature and time for RT‐MCDA‐AuNPs‐LFB were 68°C and 25 min, respectively. The limit of detection of the assay was 10 copies per test, and the specificity was 100% for the experimental strains. The whole detection procedure, including crude nucleic acid isolation (~5 min), RT‐MCDA (68°C, 25 min), and visual AuNPs‐LFB result confirmation (less than 2 min), was performed within 35 min. The preliminary results indicated that the HCV‐RT‐MCDA‐AuNPs‐LFB assay could be a valuable tool for sensitive, specific, visual, cost‐saving, and rapid detection of HCV and has potential as a POC diagnostic platform for field screening and early clinical detection of HCV infection.

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Section: Discussionmentioning

confidence: 99%