A novel PCV2 ORF5-interacting host factor YWHAB inhibits virus replication and alleviates PCV2-induced cellular response

Guo, Kangkang; Zhang, Xiuping; Hou, Yong; Liu, Jing; Feng, Quanwen; Wang, Kai; Xu, Lei; Zhang, Yanming

doi:10.1016/j.vetmic.2020.108893

Cited by 7 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five host proteins including cytochrome P450 1A1 (CYP1A1), glycoprotein-NMB (GPNMB), serine and arginine rich splicing Factor 3 (SRSF3), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein β (YWHAB), and zinc finger protein 511 (ZNF511) have been found to be able to interact with PCV2 ORF5 by yeast two-hybrid screening ( Table 1 ) [ 144 ]. Among them, both GPNMB and YWHAB can inhibit PCV2 replication by binding with ORF5 [ 150 , 151 ]. GPNMB might be involved in cell proliferation and anti-inflammatory responses [ 152 ].…”

Section: Porcine Circovirus Type2 Open Reading Frame5mentioning

confidence: 99%

Interaction between host cell proteins and open reading frames of porcine circovirus type 2

Park,

Kang

et al. 2023

J Anim Sci Technol

View full text Add to dashboard Cite

Postweaning multisystemic wasting syndrome (PMWS) is caused by a systemic inflammation after porcine circovirus type 2 (PCV2) infection. It was one of the most economically important pathogens affecting pig production worldwide before PCV2 vaccine was first introduced in 2006. After the development of a vaccine against PCV2a type, pig farms gradually restored enormous economic losses from PMWS. However, vaccine against PCV2a type could not be fully effective against several different PCV2 genotypes (PCV2b – PCV2h). In addition, PCV2a vaccine itself could generate antigenic drift of PCV2 capsid. Therefore, PCV2 infection still threats pig industry worldwide. PCV2 infection was initially found in local tissues including reproductive, respiratory, and digestive tracks. However, PCV2 infection often leads to a systemic inflammation which can cause severe immunosuppression by depleting peripheral lymphocytes in secondary lymphoid tissues. Subsequently, a secondary infection with other microorganisms can cause PMWS. Eleven putative open reading frames (ORFs) have been predicted to encode PCV2 genome. Among them, gene products of six ORFs from ORF1 to ORF6 have been identified and characterized to estimate its functional role during PCV2 infection. Acquiring knowledge about the specific interaction between each PCV2 ORF protein and host protein might be a key to develop preventive or therapeutic tools to control PCV2 infection. In this article, we reviewed current understanding of how each ORF of PCV2 manipulates host cell signaling related to immune suppression caused by PCV2.

show abstract

Section: Porcine Circovirus Type2 Open Reading Frame5mentioning

confidence: 99%

Interaction between host cell proteins and open reading frames of porcine circovirus type 2

Park,

Kang

et al. 2023

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

“…Additionally, the host transmembrane glycoprotein NMB (GPNMB) can interact with the ORF5 and restrict PCV2 infection by increasing Cyclin A and reducing of S phase of the cell cycle [ 100 ]. Moreover, 14-3-3β/α protein (also known as YWHAB, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein) can inhibit PCV2-induced ERS, autophagy, ROS, and apoptosis by directly interacting with the ORF5 protein, thus suppressing the virus infection [ 101 ].…”

Section: Crosstalk Between Pcv and Hostmentioning

confidence: 99%

Advances in Crosstalk between Porcine Circoviruses and Host

Niu

Chen

et al. 2022

Viruses

View full text Add to dashboard Cite

Porcine circoviruses (PCVs), including PCV1 to PCV4, are non-enveloped DNA viruses with a diameter of about 20 nm, belonging to the genus Circovirus in the family Circoviridae. PCV2 is an important causative agent of porcine circovirus disease or porcine circovirus-associated disease (PCVD/PCVAD), which is highly prevalent in pigs and seriously affects the swine industry globally. Furthermore, PCV2 mainly causes subclinical symptoms and immunosuppression, and PCV3 and PCV4 were detected in healthy pigs, sick pigs, and other animals. Although the pathogenicity of PCV3 and PCV4 in the field is still controversial, the infection rates of PCV3 and PCV4 in pigs are increasing. Moreover, PCV3 and PCV4 rescued from infected clones were pathogenic in vivo. It is worth noting that the interaction between virus and host is crucial to the infection and pathogenicity of the virus. This review discusses the latest research progress on the molecular mechanism of PCVs–host interaction, which may provide a scientific basis for disease prevention and control.

show abstract

“…ORF5 activates autophagy through AMPK-ERK1/2-mTOR and PERK-eIF2a-ATF4 pathways, thus promoting PCV2 replication (Lv et al, 2020). However, another study showed that ORF5 directly binds to host regulatory factor 14-3-3b (YWHAB), which inhibits ERS, ROS, and autophagy, thus inhibiting virus replication (Guo et al, 2020). In addition, PCV2 infection increases the expression of pDNAJB6 in PK-15 cells, and the C-terminal J domain of pDNAJB6 binds to the cap protein, which increases the formation of autophagy and promotes virus replication (Han et al, 2020).…”

Section: Current Knowledge Of Autophagy In Different Animal Virusesmentioning

confidence: 99%

“…Rabies virus (RABV) is a neurophagic virus that can infect people and animals and cause fatal rabies (Li et al, 2017). It is a member Increases PCV2 replication PCV2 activates autophagy through activating AMPK and increasing host oxidative stress Qian et al, 2017;Geng et al, 2020;Guo et al, 2020;Han et al, 2020;Lv et al, 2020;Zhang et al, 2020c) PCV2 induces mitophagy by increasing ROS production and the phosphorylation of Drp1 ORF5 activates autophagy through AMPK-ERK1/2-mTOR and PERK-eIF2 a-ATF4 pathways ORF5 inhibits autophagy by binding to YWHAB Cap activates autophagy through binding to pDNAJB6 Cap induces complete autophagy via inhibition of p-mTOR Pig Classical swine fever virus (CSFV)…”

Section: Porcine Rna Virusesmentioning

confidence: 99%

The Multi-Faceted Role of Autophagy During Animal Virus Infection

Jiang

Kan

Ding

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Autophagy is a process of degradation to maintain cellular homeostatic by lysosomes, which ensures cellular survival under various stress conditions, including nutrient deficiency, hypoxia, high temperature, and pathogenic infection. Xenophagy, a form of selective autophagy, serves as a defense mechanism against multiple intracellular pathogen types, such as viruses, bacteria, and parasites. Recent years have seen a growing list of animal viruses with autophagy machinery. Although the relationship between autophagy and human viruses has been widely summarized, little attention has been paid to the role of this cellular function in the veterinary field, especially today, with the growth of serious zoonotic diseases. The mechanisms of the same virus inducing autophagy in different species, or different viruses inducing autophagy in the same species have not been clarified. In this review, we examine the role of autophagy in important animal viral infectious diseases and discuss the regulation mechanisms of different animal viruses to provide a potential theoretical basis for therapeutic strategies, such as targets of new vaccine development or drugs, to improve industrial production in farming.

show abstract

A novel PCV2 ORF5-interacting host factor YWHAB inhibits virus replication and alleviates PCV2-induced cellular response

Abstract: Highlights YWHAB is a PCV2 ORF5-interacting host factor. YWHAB expression is activated by PCV2 infection and ORF5 transfection. YWHAB inhibits PCV2 replication. YWHAB alleviates PCV2 infection induced ERS, autophagy, ROS production and apoptosis.

Cited by 7 publications

References 40 publications

Interaction between host cell proteins and open reading frames of porcine circovirus type 2

Interaction between host cell proteins and open reading frames of porcine circovirus type 2

Advances in Crosstalk between Porcine Circoviruses and Host

The Multi-Faceted Role of Autophagy During Animal Virus Infection

Contact Info

Product

Resources

About