A novel peptide defined through phage display for therapeutic protein and vector neuronal targeting

Liu, James K.; Teng, Qingshan; Garrity-Moses, Mary E.; Federici, Thais; Tanase, Diana; Imperiale, Michael J.; Boulis, Nicholas M.

doi:10.1016/j.nbd.2005.01.022

Cited by 106 publications

(70 citation statements)

References 56 publications

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“…A putative strategy is gene therapy but genetic fusions are necessary for this purpose. However, it has been proposed that genetic fusion of trophic factors to larger proteins could alter their folding and conformation, thereby undermining their neuroprotective properties [13]. The aim of the present work is the production of a recombinant genetic fusion protein between BDNF and TTC and the study of BDNF functional maintenance.…”

Section: Introductionmentioning

confidence: 99%

“…The failure of these trials might be attributed in part to insufficient trophic factor delivery to motorneurons and to a non-specific delivery to non-motor systems [13]. Several authors have focused their research on the use of viral vectors transfer systems to deliver in vivo neurotrophic factors to neurons.…”

Section: Introductionmentioning

confidence: 99%

“…However, these vectors also show poor efficiency in binding motorneuron terminals [15,16] and poor specificity due to their tropism to a variety of host cells. In addition to numerous efforts to avoid virus disadvantages, some researchers involved in the adenovirus gene therapy are also proposing the use of peptides with enhanced neurotropism as an Antiapoptotic activity maintenance of Brain Derived Neurotrophic factor and the C fragment of the tetanus toxin genetic fusion protein alternative [13]. Moreover, safety concerns regarding the use of virus in humans make non viral delivery systems an attractive alternative.…”

Section: Introductionmentioning

confidence: 99%

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Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Ciriza¹,

García‐Ojeda

Martín-Burriel³

et al. 2008

Open Life Sciences

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Neurotrophic factors have been widely suggested as a treatment for multiple diseases including motorneuron pathologies, like Amyotrophic Lateral Sclerosis. However, clinical trials in which growth factors have been systematically administered to Amyotrophic Lateral Sclerosis patients have not been effective, owing in part to the short half-life of these factors and their low concentrations at target sites. A possible strategy is the use of the atoxic C fragment of the tetanus toxin as a neurotrophic factor carrier to the motorneurons.The activity of trophic factors should be tested because their genetic fusion to proteins could alter their folding and conformation, thus undermining their neuroprotective properties. For this purpose, in this paper we explored the Brain Derived Neurotrophic Factor (BDNF) activity maintenance after genetic fusion with the C fragment of the tetanus toxin. We demonstrated that BDNF fused with the C fragment of the tetanus toxin induces the neuronal survival Akt kinase pathway in mouse cortical culture neurons and maintains its antiapoptotic neuronal activity in Neuro2A cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Ciriza¹,

García‐Ojeda

Martín-Burriel³

et al. 2008

Open Life Sciences

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“…Tet1 is a 12-mer peptide, identified by phage display against the GT1b ganglioside, that displays similar binding characteristics to tetanus toxin [30]. This peptide was shown to be internalized and transported by neurons both in vitro and in vivo [31].…”

Section: Neuron Targetingmentioning

confidence: 99%

Peptide-modified vectors for nucleic acid delivery to neurons

Kwon

Bergen

Park

et al. 2008

Journal of Controlled Release

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Neuron-targeted, nucleic acid delivery systems are important technologies for realizing the potential of gene therapy for nervous system disorders. However, neurons are difficult cells to transfect using non-viral vectors due in part to the specific and unique delivery challenges present in these cells. We have investigated several bioactive peptides for their ability to assist in overcoming delivery barriers in mammalian cells. We summarize here our recent progress in developing and applying peptidemodified polycations for nucleic acid delivery. In addition, we present data demonstrating the potential of using multicomponent, peptide-modified polycations for nucleic acid delivery to neurons.

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“…17 The phage display test revealed that a 12-amino acid peptide, Tet-1, possesses a high affinity for GT1B receptor. 18,19 When plasmid DNA encoding luciferase was condensed with Tet1-polyethylene glycol (PEG)-polyethyleneimine and delivered via intracerebroventricular injection to mice, the GT1B-positive stain of transfected nerve cells was observed after delivery of Tet1-mediated vector (58%) as compared to untargeted vector (13%). 18 In most studies, two ligands modified the nanoparticles were used for cancer therapy; these ligands identified the receptors on the tumor cells so as to achieve a sequential targeting action.…”

Section: Introductionmentioning

confidence: 99%

A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice

Jia¹,

Sun²,

Lu³

et al. 2016

IJN

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Due to the impermeability of the blood–brain barrier and the nonselective distribution of drugs in the brain, the therapeutic access to intractable neurological disorders is challenging. In this study, dual brain-targeting polymersomes (POs) functionalized by transferrin and Tet-1 peptide (Tf/Tet-1-POs) promoted the transportation of curcumin into the brain and provided neuroprotection. The modification of the ligands that bind to the surface of POs was revealed by X-ray photoelectron spectroscopy analysis. The cell uptake of a coculture model of mouse brain capillary endothelial cells with neurons showed that the Tf/Tet-1-POs had significant transportation properties and possessed affinity for neurons. The pharmacokinetic analysis showed that the blood–brain barrier permeability–surface efficiency of the Tf/Tet-1-POs was 0.28 mL/h/g and that the brain tissue uptake rate (% ID/g) was 0.08, which were significant compared with the controls ( P <0.05). The curcumin-encapsulated Tf/Tet-1-POs provided neuroprotection and ameliorated cognitive dysfunction in intrahippocampal amyloid-β 1–42 -injected mice. These results suggest that the dual brain-targeting POs are more capable of drug delivery to the brain that can be exploited as a multiple noninvasive vehicle for targeting therapeutics.

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A novel peptide defined through phage display for therapeutic protein and vector neuronal targeting

Cited by 106 publications

References 56 publications

Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Peptide-modified vectors for nucleic acid delivery to neurons

A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice

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A novel peptide defined through phage display for therapeutic protein and vector neuronal targeting

Cited by 106 publications

References 56 publications

Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Antiapoptotic activity maintenance of Brain Derived Neurotrophic Factor and the C fragment of the tetanus toxin genetic fusion protein

Peptide-modified vectors for nucleic acid delivery to neurons

A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-&beta;<sub>1&ndash;42</sub>-injected mice

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A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice