A novel piezoelectric immunosensor for the detection of malarial Plasmodium falciparum histidine rich protein-2 antigen

Sharma, Mukesh; Rao, Venkat K.; Merwyn, S.; Agarwal, G. S.; Upadhyay, S. H.; Vijayaraghavan, R.

doi:10.1016/j.talanta.2011.07.008

Cited by 27 publications

(22 citation statements)

References 38 publications

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“…The characteristics of the immunosensor applied to detect HRP II are linear range of 15–60 ng/mL, minimum detection limit of 12 ng/mL, and storage stability ( t 1/2 ) of 14 days. The sensor was tested on clinical human serum samples and the results were compared with the commercially available ICT kit (NOW) which showed good agreement [119]. …”

Section: Detection Of Malariamentioning

confidence: 99%

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Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Jain

Chakma

Patra

et al. 2014

BioMed Research International

111

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Malaria has been responsible for the highest mortality in most malaria endemic countries. Even after decades of malaria control campaigns, it still persists as a disease of high mortality due to improper diagnosis and rapidly evolving drug resistant malarial parasites. For efficient and economical malaria management, WHO recommends that all malaria suspected patients should receive proper diagnosis before administering drugs. It is thus imperative to develop fast, economical, and accurate techniques for diagnosis of malaria. In this regard an in-depth knowledge on malaria biomarkers is important to identify an appropriate biorecognition element and utilize it prudently to develop a reliable detection technique for diagnosis of the disease. Among the various biomarkers, plasmodial lactate dehydrogenase and histidine-rich protein II (HRP II) have received increasing attention for developing rapid and reliable detection techniques for malaria. The widely used rapid detection tests (RDTs) for malaria succumb to many drawbacks which promotes exploration of more efficient economical detection techniques. This paper provides an overview on the current status of malaria biomarkers, along with their potential utilization for developing different malaria diagnostic techniques and advanced biosensors.

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Section: Detection Of Malariamentioning

confidence: 99%

“…The rabbit anti-PfHRP II antibodies were coupled on mixed SAM modified gold surface of quartz crystal via NHS/EDC activation method. The amount of HRP II molecules bound on the sensitive area of the electrodes is quantitatively measured as a decrease in resonant frequency [119]. …”

Section: Figurementioning

confidence: 99%

Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Jain

Chakma

Patra

et al. 2014

BioMed Research International

111

View full text Add to dashboard Cite

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“…For example, a piezoelectric immunosensor designed to bind pfHRPII in diluted human serum could detect mass changes as a shift in the resonant frequency of an antibody-functionalized quartz crystal microbalance, facilitating detection down to 12 ng ml À1 [55]. However, as is true with many mechanical sensors, there is some concern whether such a sensor exhibits the robustness necessary for a hand-held diagnostic test.…”

Section: B Nonoptical Methodsmentioning

confidence: 99%

Microfluidics and Nanotechnology for Detection of Global Infectious Diseases

Damhorst

Murtagh²,

Rodriguez

et al. 2015

Proc. IEEE

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| Infectious diseases remain among the world's leading causes of mortality and years of life lost. Significant attention has been paid to the ''Big Three'' infectious pathogensVhuman immunodeficiency virus (HIV), malaria, and tuberculosis (TB)Vbut other conditions such as Chagas' disease, dengue, Ebola, and typhoid, as well as multipathogen processes such as viral hepatitis, pneumonia, and diarrhea, also have major global impact. Addressing these significant disease burdens, which disproportionately impact the world's poorest regions, is a multifaceted grand challenge, requiring new solutions and new technologies. Diagnostics enabled by advances in microfluidics and nanotechnologies can be an important part of the solution. Advantages such as smaller sample size, increased sensitivity, and new multiplexed sensing modalities in a point-of-care format can allow rapid dissemination of test results in remote and resource-limited regions. In this review, we provide a critical assessment of the state-of-the-art in use of these technologies for detection of HIV, malaria, and TB.In addition to discussing opportunities and future prospects, we also discuss the need for additional governmental and nongovernmental funding sources to develop these technologies to their fullest potential, and the need for new business models to enable their commercialization and deployment.

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“…A further decrease in CV peak current and increased ΔE p of 208 mV was observed with the EDC/NHS activated Pt(MPA) NP modified G-MWCNT/GCE. This may be attributed to a repulsive interaction occurring in between the anionic redox probe and negatively charged terminal hydroxyl group of NHS [31]. Subsequent decreases in redox peak current were observed corresponding to the biomolecular immobilization of PtNP/G-MWCNT/GCE with anti-cTnI and its surface passivation with a blocking protein, BSA, respectively, due to insulating nature of the protein backbone chain structure [32].…”

Section: Electrochemical Characterization Of the Bioelectrodementioning

confidence: 99%

Immunoassay for troponin I using a glassy carbon electrode modified with a hybrid film consisting of graphene and multiwalled carbon nanotubes and decorated with platinum nanoparticles

et al. 2016

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This article describes a bioelectrode for the determination of human cardiac troponin-I (cTnI). A glassy carbon electrode was coated with a hybrid film of graphene and multiwalled carbon nanotube (G-MWCNT) and modified with platinum nanoparticles (Pt NPs) that were capped with mercaptopropionic acid. The PtNPs were anchored on the G-M W C N T h y b r i d f i l m v i a t h e c r o s s -l i n k e r 1 -pyrenemethylamine and subsequently functionalized with antibody against troponin (anti-cTnI). The bioelectrode was characterized by transmission electron microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The performance of the immunoelectrode was investigated by electrochemical impedance spectroscopy, and response was fit to Randle's equivalent circuit model. The charge transfer resistance (R et ) at a.c. frequencies of <1 Hz is found to be a viable sensing parameter. The dissociation constant of the immunoreaction between surface immobilized anti-cTnI and the analyte cTnI is 0.29 nM (with a Hill coefficient of 0.23), this indicating a negative cooperativity and high binding affinity of cTnI for anti-cTnI on the electrode surface. The EIS response is linear in the 1.0 pg mL −1 to 10 ng mL −1 concentration range, and the R et sensitivity is 145.5 Ω cm 2 per decade.

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A novel piezoelectric immunosensor for the detection of malarial Plasmodium falciparum histidine rich protein-2 antigen

Cited by 27 publications

References 38 publications

Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Microfluidics and Nanotechnology for Detection of Global Infectious Diseases

Immunoassay for troponin I using a glassy carbon electrode modified with a hybrid film consisting of graphene and multiwalled carbon nanotubes and decorated with platinum nanoparticles

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