A novel poly (L-lactide) degrading actinomycetes isolated from Thai forest soil, phylogenic relationship and the enzyme characterization

Characterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175

“…A total of 146 soil samples were collected from forest soils of 16 provinces in five regions of Thailand: Central region (Nakhon Sawan, Samut Prakan and Saraburi provinces), Eastern region (SaKaeo and Trat provinces), Western region (Kanchanaburi, Phetchaburi and Ratchaburi provinces), Northeastern region (Buriram, Chaiyaphum, Nakhon Ratchasima, Ubon Ratchathani and Yasothon provinces), Northern region (Lampang and Phitsanulok provinces), and Southern region (Songkhla province). Each 1 g of soil sample was suspended in 9 mL sterile distilled water, mixed well and spread on emulsified PLLA agar plates (Sukkhum et al, 2009b), and incubated at 50 C for 1 to 2 weeks. Colonies forming clear zones, i.e.…”

“…PLLA-degrading enzyme ac tivity was determined by measuring the decrease in the turbidity of emulsified PLLA, following the method of Sukkhum et al (2009b). Emulsified PLLA as the substrate was prepared by dissolving 0.1 g PLLA in 4 ml dichloromethane.…”

“…strain 41 (Pranamuda et al, 2001), Amycolatopsis sp. strain K104-1 , and Kibdelosporangium aridum ; serine protease from Tritirachium album (Williams, 1981) and Actinomadura keratinilytica strain T16-1 (Sukkhum et al, 2009b); alkaline protease from Bacillus lentus, Bacillus subtilis and Bacillus licheniformis (Oda et al, 2000); lipase (esterase) from Bacillus smithii strain PL21 (Sakai et al, 2001); lipase from Paenibacillus amylolyticus strain TB-13 (Akutsu-Shigeno et al, 2003); lipase (cutinase) from Cryptococcus sp. strain S-2 (Masaki et al, 2005); and esterase from Geobacillus thermocatenulatus (thermophilic) (Tomita et al, 2004).…”

“…strain 41 (Pranamuda et al, 2001), and Amycolatopsis orientalis ssp. orientalis (Li et al, 2008), which belong to the family Pseudonocardiaceae; Actinomadura keratinilytica strain T16-1 in the family Thermomonosporaceae (Sukkhum et al, 2009b); thermophilic Bacillus smithii in the family Bacillaceae (Sakai et al, 2001); and Paenibacillus amylolyticus strain TB-13 in the family Paenibacillaceae (Akutsu-Shigeno et al, 2003).…”

“…the families Pseudonocardiaceae (Pranamuda and Tokiwa, 1999;Pranamuda et al, 1997), Thermomonosporaceae (Sangwan and Wu, 2008;Sukkhum et al, 2009b), and Streptosporangiaceae (Sukkhum et al, 2009b). Apart from that, thermophilic bacteria in the families Thermoactinomycetaceae (Sukkhum et al, 2009b) and Firmicutes (Oda et al, 2000;Sakai et al, 2001), filamentous fungi in the genera Tritirachium and Paecilomyces (Sangwan and Wu, 2008), and yeast in the genus Cryptococcus have also been reported to be PLLA-degrading microorganisms.Many different types of enzymes are found in PLLAdegrading microorganisms, such as: protease from Amycola- …”