A novel polysaccharide, isolated from Angelica sinensis (Oliv.) Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptotic pathway

Cao, Weidong; Li, X.-Q.; Wang, X.; Fan, Hongwei; Zhang, X.-N.; Hou, Yufei; Liu, S.-B.; Mei, Qibing

doi:10.1016/j.phymed.2009.12.014

Cited by 119 publications

(52 citation statements)

References 25 publications

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“…102 Sulfated polysaccharides isolated from U. lactuca can significantly suppress the proliferation of several human cancer cells in vitro 26 and this present report suggests a potential therapeutic role of polysaccharides isolated from seaweed in the treatment of hepatocellular carcinoma. These observations are in agreement with a previous study 103 that investigated the apoptosis-inducing efficacy of polysaccharides isolated from Angelica sinensis in cervical cancer cell line. Interestingly, our results concur with previous communications which reported the antiproliferative activity of fucan sulfated polysaccharide from Sargassum filipendula against HeLa cells.…”

Section: 69supporting

confidence: 93%

Chemoprevention of Diethylnitrosamine-Initiated and Phenobarbital-Promoted Hepatocarcinogenesis in Rats by Sulfated Polysaccharides and Aqueous Extract of Ulva lactuca

et al. 2015

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Hepatocellular carcinoma (HCC) is one of the common cancers and lethal diseases worldwide. Both oxidative stress and chronic inflammation contribute to the pathogenesis of HCC. Because of limited treatment options and a grave prognosis of HCC, preventive management has been emphasized. The marine macroalgae Ulva lactuca (Ulvaceae) is consumed by humans and livestock because of its nutritional value. Recent studies showed that various extracts of U. lactuca possess antiviral, antiplasmodial, antinephrotoxic, antioxidant, and anti-inflammatory properties. However, very limited information is available on anticancer potential of U. lactuca with no reports on liver cancer chemopreventive efficacy of this marine algae. Accordingly, the present study was initiated to evaluate the possible antihepatocarcinogenic effects and antioxidant mechanisms of action of various U. lactuca extracts against a clinically relevant rodent model of HCC. Initiation of hepatocarcinogenesis was performed in Sprague-Dawley rats by a single injection of dietary carcinogen diethylnitrosamine (DENA, 200 mg/kg, intraperitoneally), followed by promotion with phenobarbital (0.05%) in drinking water. The rats were fed with daily oral dose (50 mg/kg) of polysaccharide sulfate or aqueous extract of U. lactuca for 2, 12, and 24 weeks. At these timepoints, blood samples were taken to measure hepatic injury markers, including alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transferase, and bilirubin. The liver tissue was harvested for measurement of hepatic oxidative indices, including lipid peroxidation, reduced glutathione, nitric oxide, catalase, superoxide dismutase, glutathione reductase, and glutathione S-transferase. Hepatic histopathology, immunohistochemical analysis of cell proliferation and apoptosis by DNA fragmentation assay were performed. Our results clearly indicate that sulfated polysaccharides of U. lactuca exert a marked chemoprevention of DENA-initiated hepatocarcinogenesis through inhibition of abnormal cell proliferation and induction of apoptosis. A modest inhibition rat liver carcinogenesis was observed with the aqueous extract. The sulfated polysaccharides altered serum parameters of hepatic damage and modulated various components of the hepatic enzymatic and nonenzymatic antioxidant defense systems. The sulfated polysaccharides from U. lactuca may have unique properties of providing protection against DENAinduced oxidative stress which could contribute to chemoprevention of experimental hepatocarcinogenesis. U. lactuca sulfated polysaccharides could be developed as chemopreventive and therapeutic drug against human HCC.

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Section: 69supporting

confidence: 93%

Chemoprevention of Diethylnitrosamine-Initiated and Phenobarbital-Promoted Hepatocarcinogenesis in Rats by Sulfated Polysaccharides and Aqueous Extract of Ulva lactuca

et al. 2015

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“…It has been reported that extracts of Angelica sinensis could inhibit the growth of tumor cells (33)(34)(35)(36)(37)(38)(39)(40). But, Lau et al found the water extract of Angelica sinensis stimulated the growth of MCF-7 cells (41).…”

Section: Discussionmentioning

confidence: 99%

Angelica sinensis suppresses human lung adenocarcinoma A549 cell metastasis by regulating MMPs/TIMPs and TGF-β1

Gao

Zhang²,

Zhou³

et al. 2011

Oncol Rep

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Abstract. In this study we investigated the potential effects of Angelica sinensis on the growth and metastasis in human lung adenocarcinoma A549 cells. In vitro the Cck-8 assays showed that Angelica sinensis had weak antiproliferative effect on A549 cells only at high concentration. The cell adhesion assay showed that Angelica sinensis decreased the adhesive ability of A549 cells in a dose-and time-dependent manner. Transwell invasion and migration assay showed that Angelica sinensis reduced the invasive and migratory abilities of A549 cells in a dose-dependent manner. In vivo the animal experiments showed that Angelica sinensis suppressed lung metastasis of nude mice at high concentration. Then, we attempted to clarify the mechanisms of anti-metastatic activities of Angelica sinensis. The results showed Angelica sinensis inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), it involved the down-regulation of the expressions of MMP-2 and MMP-9 at both the protein and mRNA levels, which may be associated with Angelica sinensis suppressing the expression of TGF-β1. It also involved the increase of the tissue inhibitors of metalloproteinases TIMP-2, but TIMP-1 decreased upon incubation of A549 cells with Angelica sinensis. The results suggest that Angelica sinensis might exert anti-growth and anti-metastasis activity against lung cancer cells through the decrease of MMP-2, MMP-9, TGF-β1 and TIMP-1 and increase of TIMP-2. IntroductionNon-small cell lung carcinoma (NSCLC) is currently one of the leading causes of death in China, its high mortality is attributed to early metastasis. About 70-90% patients with lung cancer need radiation therapy, and therefore, radiation lung injury is the most common complication (1). It limits the increasing of radiation dose and influences the life quality of patients or even threatens their life. Many traditional Chinese medicines have great efficacy in radiation lung injury. The root of Angelica sinensis, also known as 'Danggui' was a popular herbal medicine and widely used for many diseases in China. It has been used for more than 10 years in our hospital to treat lung fibrosis and shows good curative effect in radiation pulmonary fibrosis (2-4).Angelica sinensis belongs to 'activating blood and dissolving stasis' kind of traditional Chinese medicine, and some researchers think this kind of medicine can promote tumor metastasis. Li et al first reported Salvia miltiorrhiza Bunge and Radix Paeoniae Rubra such as 'activating blood and dissolving stasis' traditional Chinese medicine can promote tumor metastasis, we pay high attention to it (5). Han et al found the metastasis rate of nasopharyngeal carcinoma increased to 2.67 times compare with the control after using 'activating blood and dissolving stasis' kind of traditional Chinese medicine (6). But also, there is research with the opposite conclusion (7). Therefore, the use of Angelica sinensis needs to be clarified to confirm whether it promotes or inhibits metastasis of l...

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“…Diels (Umbelliferae) [2], a well-known Chinese herbal medicine, first documented in Shennong Bencao Jing (Shennong's Classic of Materia Medica; 200–300 AD) [3, 4]. It has been used historically as a tonic, hematopoietic, and anti-inflammatory agent for the treatment of gynecological diseases such as menstrual disorders, amenorrhea, and dysmenorrheal for thousands of years in traditional Chinese Medicinal prescriptions [5, 6]. It has also been widely marketed as health food for women's care in Asia [7] and as a dietary supplement in Europe and America [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Correlation between Commodity Grade and Quality of Angelica sinensis by Determination of Active Compounds Using Ultraperformance Liquid Chromatography Coupled with Chemometrics

Wang

Huang

2014

Evidence-Based Complementary and Alternative Medicine

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The contents of ferulic acid, senkyunolide A, butylidenephthalide, ligustilide, and n-butylphthalide were determined by UPLC analytical method; the correlation among the grade, average weight, and content was explored by correlation analysis and analysis of variance (ANOVA); the different commercial grades with average weight and content were revealed by principal component analysis (PCA) and then rationality analysis grade classification of A. sinensis. The results showed that various commercial grades can be distinguished by PCA analysis. And there was significant negative correlation between the commodity grades and average weight, commodity, and the content of bioactive compounds, while the content of senkyunolide A had significant negative correlation with commodity grades (P < 0.01). Average weight had no correlation with chemicals compounds. Additionally, there was significant positive correlation among the bioactive compounds (content of ferulic acid and phthalides) of different grades of A. sinensis. The content of senkyunolide A, butylidenephthalide, and ligustilide had significant positive correlation with the content of ferulic acid. The content of ligustilide and butylidenephthalide had significant positive correlation with the content of senkyunolide A. The content of ligustilide had significant positive correlation with the content of butylidenephthalide. The basis of grades classification is related with the difference levels of the bioactive compounds.

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A novel polysaccharide, isolated from Angelica sinensis (Oliv.) Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptotic pathway

Cited by 119 publications

References 25 publications

Chemoprevention of Diethylnitrosamine-Initiated and Phenobarbital-Promoted Hepatocarcinogenesis in Rats by Sulfated Polysaccharides and Aqueous Extract of Ulva lactuca

Chemoprevention of Diethylnitrosamine-Initiated and Phenobarbital-Promoted Hepatocarcinogenesis in Rats by Sulfated Polysaccharides and Aqueous Extract of Ulva lactuca

Angelica sinensis suppresses human lung adenocarcinoma A549 cell metastasis by regulating MMPs/TIMPs and TGF-β1

Analysis of the Correlation between Commodity Grade and Quality of Angelica sinensis by Determination of Active Compounds Using Ultraperformance Liquid Chromatography Coupled with Chemometrics

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