A novel preadipocyte cell line established from mouse adult mature adipocytes

Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

“…To our study, DFAT cells remained stable and strong proliferation ability after 30th passage without detectable establishment of lipid droplet inside plasma, which was consistent with other reports [24]. PCR analysis showed that although DFAT cells had decreased level of LPL, leptin and glucose transporter 4 (GLUT4) compared with mature adipocytes, they still express key adipogenic markers including peroxisome proliferator-activated receptor gamma (PPARγ), CAAT/ enhancer-binding proteins (C/EBPα, C/EBPβ and C/EBPδ) and sterol regulatory element-binding protein-1c (SREBP1c) [14,[24][25]. In vitro, several recipes of adipogenic cultures were applied with different combination and dosage of induction chemicals such as dexamethasone, 3-isobutyl-1-methylxanthine and insulin transferring selenium X (ITS) [14][15]18].…”

“…According to the different studies [14, 16-17, 19, 23], in the following 2 to 3 weeks of uninterrupted culture, the fat drops within the adipocytes will gradually disappear and the cells shift into fibroblast-like morphology, reestablishing a strong proliferation ability as well. Distinct to the ASCs, DFAT cells derived from ceiling culture method were identified as a more homogenous cell group in most reports [16][17][18][19][23][24][25][26]. Although, this simple technique needs no complex procedures such as transfection or chemical induction, several key points should still be paid attention to.…”

“…In vivo experiment showed same adipogenic capacity of DFAT cells. Direct injection of DFAT cell suspension by syringes into the subcutaneous portion over sternum of mice resulted in fat pad formation free of chemical induction after 3 weeks [24][25].…”

Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation

“…12). Unlike 3T3-L1 cells, these DFAT cells do not undergo spontaneous adipogenesis, with the adipocytic differentiation of DFAT cells thus being more tightly controlled than that of 3T3-L1 cells 12,13 . Here, we examine the roles of actin cytoskeleton remodelling in adipocytic differentiation by using DFAT cells.…”

Cloning of Homozygous a1,3-Galactosyltransferase Gene Knock-Out Pigs by Somatic Cell Nuclear Transfer

“…The ability of the obtained preadipocytes to re-differentiate into mature adipocytes was confirmed by the induction of differentiation in vitro (Tomii et al, 2005;Yagi et al, 2004). Sub-confluent preadipocytes were cultured for 4 days in DMEM supplemented with 20% (v/v) FBS, 0.5 mM 3-isobutyl-1-methylxanthine, 5 μg/mL insulin and 0.25 μM dexamethasone to induce adipogenic differentiation.…”