A novel primary amine-based anion exchange membrane adsorber

Woo, Maybelle; Khan, Navid; Royce, Jonathan; Mehta, Ushma; Gagnon, Brian A.; Ramaswamy, Senthil; Soice, Neil; Morelli, Michele; Cheng, Kwok-Shun

doi:10.1016/j.chroma.2011.03.068

Cited by 34 publications

(12 citation statements)

References 20 publications

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“…Lower total binding capacities of the MQ and SQ membranes compared to the CS membrane can thus not be inferred unerringly. For comparison, binding capacities for other viruses to strong anion exchange membranes have been reported previously in a similar range (Han et al, 2005;Kutner et al, 2009;Woo et al, 2011;Wu et al, 2007).…”

Section: Virus Adsorptionsupporting

confidence: 58%

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Grein

Michalsky

López

et al. 2012

Journal of Virological Methods

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Section: Virus Adsorptionsupporting

confidence: 58%

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Grein

Michalsky

López

et al. 2012

Journal of Virological Methods

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“…ChromaSorb showed a fourfold higher static binding capacity than Sartobind Q and a 12‐fold higher capacity than Mustang Q. While Sartobind Q and Mustang Q contain quaternary amine based anion‐exchange ligands, ChromaSorb contains polyallylamine (primary amine based ligands) (Woo et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Anion exchange membrane adsorbers for flow‐through polishing steps: Part I. clearance of minute virus of mice

Weaver

Husson

Murphy

et al. 2012

Biotech & Bioengineering

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Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval for changes to a manufacturing process. This study has investigated clearance of minute virus of mice (MVM), an 18-22 nm parvovirus recognized by the FDA as a model viral impurity. Virus clearance was obtained using three commercially available anion exchange membrane adsorbers: Sartobind Q®, Mustang Q®, and ChromaSorb®. Unlike earlier studies that have focused on a single or few operating conditions, the aim here was to determine the level of virus clearance under a range of operating conditions that could be encountered in industry. The effects of varying pH, NaCl concentration, flow rate, and other competing anionic species present in the feed were determined. The removal capacity of the Sartobind Q and Mustang Q products, which contain quaternary ammonium based ligands, is sensitive to feed conductivity and pH. At conductivities above about 20 mS/cm, a significant decrease in capacity is observed. The capacity of the ChromaSorb product, which contains primary amine based ligands, is much less affected by ionic strength. However the capacity for binding MVM is significantly reduced in the presence of phosphate ions. These differences may be explained in terms of secondary hydrogen bonding interactions that could occur with primary amine based ligands.

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“…STIC chromatography membranes (Sartorius Stedim Biotech) are examples of commercially available products based on primary amine ligands. There is, however, still a limited number of published work on the performance analysis of these novel chromatographic ligands (Kang et al, ; Woo et al, ). Moreover, no attempts to use membrane chromatography in flow‐through mode for virus purification have been reported in the open literature.…”

Section: Chromatographymentioning

confidence: 99%

Improved virus purification processes for vaccines and gene therapy

Nestola

Peixoto

Silva

et al. 2015

Biotech & Bioengineering

113

106

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The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral downstream purification processes, encompassing the classical unit operations and their recent developments. Emphasis is given to novel strategies for process intensification, such as continuous or semi-continuous systems based on multicolumn technology, opening up process efficiency. Process understanding in the light of the pharmaceutical quality by design (QbD) initiative is also discussed. Finally, an outlook of the upcoming breakthrough technologies is presented.

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A novel primary amine-based anion exchange membrane adsorber

Cited by 34 publications

References 20 publications

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Anion exchange membrane adsorbers for flow‐through polishing steps: Part I. clearance of minute virus of mice

Improved virus purification processes for vaccines and gene therapy

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