A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii

Abstract

One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SKENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.

Enhancement of feather degrading keratinase of Streptomyces swerraensis KN23, applying mutagenesis and statistical optimization to improve keratinase activity

“…DNA extraction: The extraction method was applied according to the manufacturer of (Applied Biotechnology Co., Egypt), the actinomyces isolate was identified based on partial sequencing of the 16S rRNA gene, using the universal primers as follow, forward primer 63f (5’- CAGGCCTAACACATGCAAGTC-3’) and reverse primer 1387R (5’- GGGCGGWGTGTACAAGGC-3’). PCR product was purified and sequenced as described previously [ 28 ]. PCR program was 95 °C for 3 min, 30 cycles of denaturation at 95 °C for 45 s, annealing at 56 °C for 45 s, and extension at 72 °C for 1 min /1kbp, final extension 72 °C for 5 min.…”

Towards an iSUCCELL Saccharomyces cerevisiae platform for fuel ethanol production

“…M. guilliermondii is a typical aerobic yeast, with a standard growth temperature of 30 °C, that cannot grow under strict anaerobic conditions. Its ability to utilise cellobiose as carbon source is well-described (Freer 1991;Rodríguez et al 2004;Khattab and Kodaki 2016;da Silveira et al 2020;Mo et al 2021), but reports differ regarding its capacity to catabolise cellobiose via fermentation, suggesting that this property is strain-dependent (Freer 1991;Khattab and Kodaki 2016;Lopes et al 2018).…”