A Novel Protein- and Hormone-Free Medium: Its Application to Hybridoma Culture and Monoclonal Antibody Production

Inouye, Kuniyo; Toyoda, Kazuhisa; Kondo, Masahide; Maki, Koichi

doi:10.1007/978-94-011-2844-5_48

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Effect Of Culture Conditions On Hybridoma Growth and Antibody1

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1993

2009

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Cited by 4 publications

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“…For these reasons a number of serum-free and proteinfree defined media have been developed. (Barnes and Sato, 1980;Franek et aL, 1992;Inouye et al, 1992). The success of these media is very much dependent on the individual requirements of cell lines.…”

Section: Effect Of Culture Conditions On Hybridoma Growth and Antibodymentioning

confidence: 99%

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Venables

1993

Animal Cell Technology: Basic &Amp; Applied Aspects

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Section: Effect Of Culture Conditions On Hybridoma Growth and Antibodymentioning

confidence: 99%

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Venables

1993

Animal Cell Technology: Basic &Amp; Applied Aspects

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Cell Products—Antibodies

Birch¹

2000

Encyclopedia of Cell Technology

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Cell Products: Antibodies

Birch

2009

Encyclopedia of Industrial Biotechnology

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The applications of monoclonal antibodies are now widespread, as research tools, as in vitro diagnostic reagents and, increasingly, as in vivo diagnostic and therapeutic products. The number of antibody therapeutics on the market and in clinical trials has increased rapidly in recent years and global production has now reached tonnage quantities. Manufacturing technology is for the most part based on mammalian cell culture in fed‐batch suspension culture. Improvements in cell‐line construction and in media and feeds have led to dramatic improvements in productivity. Titers of grams per liter are now seen routinely and, increasingly, this is being achieved in media that are chemically defined and protein‐free. The requirement for very large quantities of antibody products also presents a challenge in terms of purification processes. Currently, purification is based on column chromatography, typically with a Protein‐A affinity step early in the process. In addition to optimizing processes for productivity, there is a growing awareness of the need to design cells and processes to deliver products with desired structural features.

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A Novel Protein- and Hormone-Free Medium: Its Application to Hybridoma Culture and Monoclonal Antibody Production

Cited by 4 publications

References 10 publications

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Cell Products—Antibodies

Cell Products: Antibodies

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