A Novel Pyroptosis-Associated Long Non-coding RNA Signature Predicts Prognosis and Tumor Immune Microenvironment of Patients With Breast Cancer

Abstract

Background: Pyroptosis, a kind of programmed cell death characterized by the rupture of cell membranes and the release of inflammatory substances, plays an important role in the occurrence and development of cancer. However, few studies focus on the pyroptosis-associated long non-coding RNAs (lncRNAs) in breast cancer (BC). The prognostic value of pyroptosis-associated lncRNAs and their relationship with tumor microenvironment (TME) in BC remain unclear. The purpose of this study was to explore the prognostic role of pyroptosis-associated lncRNAs and their relationship with TME in BC.Methods: The transcriptome data and clinical data of female BC patients were downloaded from The Cancer Genome Atlas (TCGA) database. A total of 937 patients were randomly assigned to either training set or validation set. A pyroptosis-associated lncRNA signature was constructed in the training set and verified in the validation set. Functional analysis and immune microenvironment analysis related to pyroptosis-associated lncRNAs were performed. A nomogram based on the risk score and clinical characteristics was established.Results: A 9-pyroptosis-associated lncRNA signature was constructed to separate BC patients into two risk groups. High-risk patients had poorer prognosis than low-risk patients. The risk score was proven to be an independent prognostic factor by multivariate Cox regression analysis. Function analysis and immune microenvironment analysis showed that low-risk BC tended to be an immunologically “hot” tumor. A nomogram was constructed with risk score and clinical characteristics. Receiver operating characteristic curve (ROC) analysis demonstrated credible predictive power of the nomogram. The area under time-dependent ROC curve (AUC) reached 0.880 at 1 year, 0.804 at 3 years, and 0.769 at 5 years in the training set, and 0.799 at 1 year, 0.794 at 3 years, and 0.728 at 5 years in the validation set.Conclusion: We identified a novel pyroptosis-associated lncRNA signature that was an independent prognostic indicator for BC patients. Pyroptosis-associated lncRNAs had potential relationship with the immune microenvironment and might be therapeutic targets for BC patients.

Prognosis Risk Model Based on Pyroptosis-Related lncRNAs for Bladder Cancer

“…Due to the rapid development of bioinformatics in recent years, sifting pyroptosis-related mRNA or lncRNA as prognostic signatures has become a reality, which is helpful to understand their role in the occurrence, development, and prognosis of carcinoma and provide new ideas for clinical prevention and treatment [ 10 , 11 ]. In the current study, we downloaded and analysed a dataset of lncRNA expression in bladder cancer from The Cancer Genome Atlas (TCGA).…”

A Comprehensive Analysis of Pyroptosis-Related lncRNAs Signature Associated With Prognosis and Tumor Immune Microenvironment of Pancreatic Adenocarcinoma

“…Meanwhile, the activation of pyroptosis tends to be accompanied by a severe inflammatory response that may affect the TIME; therefore, like other forms of PCDs such as apoptosis, autophagy, and ferroptosis, pyroptosis has become one of the hot topics in current cancer research ( Hsu et al, 2021 ). Recent studies have investigated the prognostic significance of pyroptosis and its connections with the TIME ( Ping et al, 2021 ; Sun et al, 2021 ; Wu et al, 2021 ; Ye et al, 2021 ). However, whether pyroptosis is correlated with the prognosis or specific microenvironment of patients with PAAD or not remains unclear.…”

Pyroptosis-Related lncRNAs for Predicting the Prognosis and Identifying Immune Microenvironment Infiltration in Breast Cancer Lung Metastasis

“…A recent study has also confirmed the pivotal role of PR in the anti-tumor function of NK cells (Zhang et al, 2020b). Ping, Liqin and Lv W et al also demonstrated that PR-associated lncRNAs serve a prognostic role in breast cancer as well as its correlation with TME (Lv et al, 2021;Ping et al, 2021). Therefore, PR plays an important role in tumor development and the antitumor process (Wu et al, 2021); however, its specific function in BC remains to be further elucidated.…”