A novel quantitative trait locus for Fusarium head blight resistance in chromosome 7A of wheat

Abstract

A Chinese Spring-Sumai 3 chromosome 7A disomic substitution line (CS-Sumai 3-7ADSL) was reported to have a high level of Fusarium head blight (FHB) resistance for symptom spread within a spike (Type II) and low deoxynivalenol accumulation in infected kernels (Type III), but a quantitative trait locus (QTL) on chromosome 7A has never been identified from this source. To characterize QTL on chromosome 7A, we developed 191 7A chromosome recombinant inbred lines (7ACRIL) from a cross between Chinese Spring and CS-Sumai 3-7ADSL and evaluated both types of resistance in three greenhouse experiments. Two major QTL with Sumai 3 origin, conditioning both Type II and III resistance, were mapped in the short arm of chromosomes 3B (3BS) and near the centromere of chromosome 7A (7AC). The 3BS QTL corresponds to previously reported Fhb1 from Sumai 3, whereas 7AC QTL, designated as Fhb7AC, is a novel QTL identified from CS-Sumai 3-7ADSL in this study. Fhb7AC explains 22% phenotypic variation for Type II and 24% for Type III resistance. Marker Xwmc17 is the closest marker to Fhb7AC for both types of resistance. Fhb1 and Fhb7AC were additive, and together explained 56% variation for Type II and 41% for Type III resistance and resulted in 66% reduction in FHB severity and 84% reduction in deoxynivalenol (DON) content. Haplotype analysis of Sumai 3 parents revealed that Fhb7AC originated from Funo, an Italian cultivar. Fhb7AC has the potential to be used in improving wheat cultivars for both types of resistance.

Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu

“…Xbarc121. This result suggests that the 7AL QTL may be the same QTL as previously 21 reported in these various lines. …”

QTL mapping and successful introgression of the spring wheat-derived QTL Fhb1 for Fusarium head blight resistance in three European triticale populations

“…In 2011, Jayatilake et al reported a QTL from CS-Sumai 3-7ADSL with a high level of FHB resistance for symptom spread within a spike (type 2) and low deoxynivalenol accumulation in infected kernels (type 3). Designated as Fhb7AC, this QTL mapped near the centromere of the chromosome 7A and explained a similar level of resistance than the QTL detected in this study on chromosome 7A (22% phenotypic variation for type 2 and 24% for type 3 resistance, Jayatilake et al 2011). Further testing will be necessary to uncover whether or not those two QTLs are identical or at proximity.…”

Whole Genome Association Mapping of Fusarium Head Blight Resistance in European Winter Wheat (Triticum aestivum L.)

“…Therefore during the last decade a number of quantitative trait loci (QTL) mapping studies using molecular markers were conducted. The first mapped QTL originated from the Chinese spring wheat accession ‘Sumai 3’ or its derivatives [1]–[3]] and later efforts were undertaken to integrate these QTL into adapted germplasm [4]–[6]. This was followed by the identification and mapping of QTL for Fusarium resistance and mycotoxin accumulation in a number of bi-parental winter wheat populations [7]–[13], as well as efforts to introduce the novel QTL into elite background [14].…”