A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode

Wu, Ying; Zheng, Junwei; Li, Zheng; Zhao, Yanrong; Zhang, Yu

doi:10.1016/j.bios.2008.07.075

Cited by 61 publications

(14 citation statements)

References 20 publications

(19 reference statements)

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“…866 A polymer-membrane based potentiometric IgG sensing method using silver enlargement of AuNPs tracers was reported by Chumbimuni-Torres et al . to detect mouse IgG with a detection limit of ~12.5 pmol in the 50 μL sample.…”

Section: Electrical and Electrochemical Sensingmentioning

confidence: 99%

Gold Nanoparticles in Chemical and Biological Sensing

et al. 2012

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Section: Electrical and Electrochemical Sensingmentioning

confidence: 99%

Gold Nanoparticles in Chemical and Biological Sensing

et al. 2012

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“…The resulting immunosensor showed a linear amperomatric response in the concentration range of 4–120 ng mL −1 and the detection limit was estimated to be 1.0 ng mL −1 . This immunosensor retained 90% of the initial current response after 30 days [89]. In another strategy, self-assembled gold nanoparticle monolayers were developed on the working electrode for amperomatric detection of α-1-fetoprotein in human serum samples.…”

Section: Application Of Nanostructured Materials To Immunosensorsmentioning

confidence: 99%

Prospects of Nanotechnology in Clinical Immunodiagnostics

Ansari

Alhoshan

AlSalhi

et al. 2010

Sensors

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Nanostructured materials are promising compounds that offer new opportunities as sensing platforms for the detection of biomolecules. Having micrometer-scale length and nanometer-scale diameters, nanomaterials can be manipulated with current nanofabrication methods, as well as self-assembly techniques, to fabricate nanoscale bio-sensing devices. Nanostructured materials possess extraordinary physical, mechanical, electrical, thermal and multifunctional properties. Such unique properties advocate their use as biomimetic membranes to immobilize and modify biomolecules on the surface of nanoparticles. Alignment, uniform dispersion, selective growth and diameter control are general parameters which play critical roles in the successful integration of nanostructures for the fabrication of bioelectronic sensing devices. In this review, we focus on different types and aspects of nanomaterials, including their synthesis, properties, conjugation with biomolecules and their application in the construction of immunosensing devices. Some key results from each cited article are summarized by relating the concept and mechanism behind each sensor, experimental conditions and the behavior of the sensor under different conditions, etc. The variety of nanomaterial-based bioelectronic devices exhibiting novel functions proves the unique properties of nanomaterials in such sensing devices, which will surely continue to expand in the future. Such nanomaterial based devices are expected to have a major impact in clinical immunodiagnostics, environmental monitoring, security surveillance and for ensuring food safety.

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“…Nowadays, several direct immunosensors based on electrochemical transducers, such as potentiometric (Taylor, Marenchic, & Spencer, 1991), amperometric (Tang et al, 2005), conductimetric (Yagiuda, Hemmi, Ito & Asano, 1996), and impedimetric (Rahman, Shiddiky, Park & Shim, 2007), have been developed. Among the various types of electrochemical immunosensors mentioned above, the electrochemical impedimetric immunosensor has been extensively investigated in the fields of biological studies (Escamilla-Gómez, Campuzano, Pedrero & Pingarrón, 2009;Huang et al, 2010;Radi, Muñoz-Berbel, Lates & Marty, 2009) and applied to the detection of drug residues in food during recent years (Ionescu et al, 2007;Thavarungkul, Dawan, Kanatharana & Asawatreratanakul, 2007;Wu, Zheng, Li, Zhao & Zhang, 2009). For example, Nicole Jaffrezic-Renault et al reported the detection of trace amounts of ciprofloxacin antibiotic by impedimetric immunosensor (Ionescu et al, 2007).…”

Section: Introductionmentioning

confidence: 98%