A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression

Zhang, Xinyang; Cheng, Baoping; Liu, Chang; Du, Zhiqiang; Zhang, Hui; Wang, Ning; Wu, Mengqi; Li, Yumao; Zhi, Cao; Li, Hui

doi:10.3389/fphys.2019.00458

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…The thymidine kinase ( TK ) minimal promoter with 3 copies of the DR1 element (AGGTCAAAGGTCA) at its 5′ end was synthesized by GENEWIZ and cloned into the pGL3-Basic Luciferase Reporter Vector (Promega) to yield the 3xPPRE-TK-Luc reporter construct. The CCAAT/enhancer-binding protein alpha ( C/EBPα ), fatty acid synthase ( FAS ), adipocyte fatty acid–binding protein ( A-FABP ), lipoprotein lipase ( LPL ), and perilipin 1 ( PLIN1 ) promoter reporters containing the potential PPRE were previously generated by our laboratory ( Zhang et al., 2014 ; Zhang et al., 2019 ). The full-length PPARγ1 (NM_001001460.1) was amplified from the pooled chicken cDNA using PCR with the following primers: PPARγ1 -F (5′-GGAATTCATGGTTGACACAGAAATGCCGT-3′) and PPARγ1 -R (5′-CCTCGAGGAGGATAAGAACTACTATCGCC-3′) and introduced into the Eco RI and Xho I sites of a pCMV-HA vector to yield pCMV-HA-PPARγ1.…”

Section: Methodsmentioning

confidence: 99%

Peroxisome proliferator-activated receptor γ isoforms differentially regulate preadipocyte proliferation, apoptosis, and differentiation in chickens

Yang

Ning

et al. 2020

Poultry Science

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Peroxisome proliferator-activated receptor γ ( PPARγ ) has 2 protein isoforms ( PPARγ1 and PPARγ2 ) generated by alternative promoter usage and alternative splicing. However, their functional uniqueness and similarity remain unclear. In the study, we investigated the effects of lentivirus-mediated overexpression of PPARγ1 and PPARγ2 on proliferation, apoptosis, and differentiation of the immortalized chicken preadipocytes. Cell Counting Kit–8 assay showed PPARγ1 and PPARγ2 overexpression markedly suppressed cell proliferation, and fluorescence activated cell sorting analysis showed that PPARγ1 and PPARγ2 overexpression caused cell cycle arrest at G0/G1 phase. Cell death detection ELISA analysis showed both PPARγ1 and PPARγ2 overexpression induced cell apoptosis. Oil red O staining and gene expression analysis showed both PPARγ1 and PPARγ2 overexpression promoted preadipocyte differentiation. In the presence of PPARγ ligand, rosiglitazone, PPARγ2 overexpression was more potent in inducing apoptosis, promoting adipogenesis, and suppressing cell proliferation than PPARγ1 overexpression. We further explored the molecular basis for their functional differences. Reporter gene assay showed that under ligand conditions, PPARγ2 overexpression resulted in 1.68-fold increase in transcription activity compared with PPARγ1. Electrophoretic mobility shift assay showed both PPARγ1 and PPARγ2 could bind to PPAR response element ( PPRE ) as heterodimer with retinoid X receptor alpha, and by comparison, PPARγ2 had a higher affinity for PPRE than PPARγ1. Reporter gene assay showed expression PPARγ1 and PPARγ2 similarly induced fatty acid synthase and adipocyte fatty acid–binding protein promoter activity but differentially induced lipoprotein lipase and perilipin 1 promoter activities. Coimmunoprecipitation analysis showed that PPARγ1 and PPARγ2 interacted similarly with the coactivators, Tat-interacting protein 60. Taken together, our results demonstrate that PPARγ1 and PPARγ2 differentially regulate preadipocyte proliferation, apoptosis, and differentiation as a result of their distinct and overlapping molecular functions.

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Section: Methodsmentioning

confidence: 99%

Peroxisome proliferator-activated receptor γ isoforms differentially regulate preadipocyte proliferation, apoptosis, and differentiation in chickens

Yang

Ning

et al. 2020

Poultry Science

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“…In vitro , Tcf21 increased pre-adipocyte differentiation, whereas Tcf21 knockdown and overexpression attenuated and promoted pre-adipocyte differentiation, respectively, which changed lipid droplet accumulation. Moreover, ChIP and luciferase analyses showed that TCF21 controls the transcription of lipoprotein lipase (LPL) by directly binding to the E-box motif in the Lpl promoter ( 62 ).…”

Section: Tcf21 In the Development Of Other Tissuesmentioning

confidence: 99%

Role of the bHLH transcription factor TCF21 in development and tumorigenesis

Lotfi

Passaia

Kremer

2021

Braz J Med Biol Res

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Transcription factors control, coordinate, and separate the functions of distinct network modules spatially and temporally. In this review, we focus on the transcription factor 21 (TCF21) network, a highly conserved basic-helix-loop-helix (bHLH) protein that functions to integrate signals and modulate gene expression. We summarize the molecular and biological properties of TCF21 control with an emphasis on molecular and functional TCF21 interactions. We suggest that these interactions serve to modulate the development of different organs at the transcriptional level to maintain growth homeostasis and to influence cell fate. Importantly, TCF21 expression is epigenetically inactivated in different types of human cancers. The epigenetic modification or activation and/or loss of TCF21 expression results in an imbalance in TCF21 signaling, which may lead to tumor initiation and, most likely, to progression and tumor metastasis. This review focuses on research on the roles of TCF21 in development and tumorigenesis systematically considering the physiological and pathological function of TCF21. In addition, we focus on the main molecular bases of its different roles whose importance should be clarified in future research. For this review, PubMed databases and keywords such as TCF21, POD-1, capsulin, tumors, carcinomas, tumorigenesis, development, and mechanism of action were utilized. Articles were selected within a historical context as were a number of citations from journals with relevant impact.

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“…Therefore, chicken was considered a more suitable model for non-alcoholic fatty liver disease (NAFLD). Enough studies have revealed the details about lipid synthesis, especially the enzymes of lipid synthesis, such as ACC, FAS, and LPL (Mottillo et al, 2017;Seo et al, 2018;Zhang et al, 2019;Bhat et al, 2021). Meanwhile, many studies have concluded that adult chicken fat tissue has no or very weak lipid synthesis ability (Na et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period

Zhao

Tang

et al. 2021

Front. Physiol.

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Since excess abdominal fat is one of the main problems in the broiler industry for the development of modern broiler and layer industry, the importance of subcutaneous adipose tissue has been neglected. However, chick subcutaneous adipose tissue appeared earlier than abdominal adipose tissue and more than abdominal adipose tissue. Despite a wealth of data, detailed information is lacking about the development and function of chick subcutaneous adipose tissue during the embryonic and posthatch period. Therefore, the objective of the current study was to determine the developmental changes of adipocyte differentiation, lipid synthesis, lipolysis, fatty acid β-oxidation, and lipid contents from E12 to D9.5. The results showed that subcutaneous adipose tissue was another important energy supply tissue during the posthatch period. In this stage, the mitochondrial copy number and fatty acid β-oxidation level significantly increased. It revealed that chick subcutaneous adipose tissue not only has the function of energy supply by lipidolysis but also performs the same function as brown adipose tissue to some extent, despite that the brown adipose tissue does not exist in birds. In addition, this finding improved the theory of energy supply in the embryonic and posthatch period and might provide theoretical basis on physiological characteristics of lipid metabolism in chicks.

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A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression

Cited by 16 publications

References 28 publications

Peroxisome proliferator-activated receptor γ isoforms differentially regulate preadipocyte proliferation, apoptosis, and differentiation in chickens

Peroxisome proliferator-activated receptor γ isoforms differentially regulate preadipocyte proliferation, apoptosis, and differentiation in chickens

Role of the bHLH transcription factor TCF21 in development and tumorigenesis

Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period

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