A novel role for fatty acid transport protein 1 in the regulation of tricarboxylic acid cycle and mitochondrial function in 3T3-L1 adipocytes

Wiczer, Brian M.; Bernlohr, David A.

doi:10.1194/jlr.m900218-jlr200

Cited by 26 publications

(18 citation statements)

References 60 publications

(85 reference statements)

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“…B and 2B). In our hands, only Slc27a1 was induced by Rosi, which might relate to roles of the product of this gene (fatty acid transport protein 1) in facilitating fatty acid uptake and regulating mitochondrial function (Wiczer and Bernlohr, ). All in all, our results suggest that brite cells may be different from beige cells and that brown‐like adipogenesis and beige adipogenesis may not be the same.…”

Section: Discussionmentioning

confidence: 83%

Cell‐Autonomous Brown‐Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice

Petrov

Palou

Bonet

et al. 2016

Journal Cellular Physiology

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Mechanisms behind the emergence of brown adipocyte-like (brite or beige) adipocytes within white adipose tissue (WAT) are of interest. Retinoblastoma protein gene (Rb) haploinsufficiency associates in mice with improved metabolic regulation linked to a greater capacity for fatty acid oxidation and thermogenesis in WAT. We aimed to explain a feasible mechanism of WAT-to-BAT remodeling in this model. Differentiated primary adipocytes and Sca1-positive preadipocytes derived from adipose depots of Rb(+/-) mice and wild-type siblings were compared. Primary white Rb(+/-) adipocytes displayed under basal conditions increased glucose uptake and an enhanced expression of brown adipocyte-related genes (Pparg, Ppargc1a, Ppargc1b, Prdm16, Cpt1b) but not of purported beige/brite transcriptional markers (Cd137, Tmem26, Tbx1, Slc27a1, Hoxc9, Shox2). Lack of induction of beige markers phenocopied results in WAT of adult Rb(+/-) mice. Flow cytometry analysis evidenced an increased number of preadipocytes in WAT depots of Rb(+/-) mice. Sca1(+) preadipocytes from WAT of Rb(+/-) mice displayed increased gene expression of several transcription factors common to the brown and beige adipogenic programs (Prdm16, Pparg, Ppargc1a) and of receptors of bone morphogenetic proteins (BMPs); however, among the recently proposed beige markers, only Tbx1 was upregulated. Adult Rb(+/-) mice had increased circulating levels of BMP7. These results indicate that preadipose cells resident in WAT depots of Rb(+/-) mice retain an increased capacity for brown-like adipogenesis that appears to be different from beige adipogenesis, and suggest that the contribution of these precursors to the Rb(+/-) adipose phenotype is driven, at least in part, by interaction with BMP7 pathways. J. Cell. Physiol. 231: 1941-1952, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 83%

Cell‐Autonomous Brown‐Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice

Petrov

Palou

Bonet

et al. 2016

Journal Cellular Physiology

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“…Excess acetylcarnitine could generate more acetyl‐CoA, leading to a higher acetyl‐CoA:CoA ratio, which inhibited pyruvate dehydrogenase and consequently reduced the activity of the TCA cycle (Rosca et al ., ). There was a correlation between decreased TCA flow and increased fatty acid oxidation in 3T3‐L1 adipocytes (Wiczer and Bernlohr, ), and this is a possible mechanism explaining the better effect of acetylcarnitine than carnitine in reducing adipogenesis in ASCs.…”

Section: Discussionmentioning

confidence: 97%

Carnitine and acetylcarnitine modulate mesenchymal differentiation of adult stem cells

Zhang

Elisseeff

2013

J Tissue Eng Regen Med

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Cellular metabolic activity, especially mitochondrial metabolism, plays a vital role in regulating cell proliferation and differentiation. Metabolism could therefore be an important factor to consider when using engineering technologies to stimulate tissue development and repair. The small metabolite carnitine and its derivative acetylcarnitine affect the activities of several pathways in mitochondrial metabolism, but their influence on cell differentiation has not yet been thoroughly studied. To elucidate the effects of these two small molecules on mesenchymal tissue engineering, we used adult stem cells as a platform in both monolayer and 3D hydrogel culture systems. We investigated the impact of these two small molecules on the differentiation of adult stem cells and analysed gene expression, cell proliferation and extracellular matrix deposition. We found that the molecules reduced adipogenesis but stimulated osteogenesis and chondrogenesis in both culture systems. Our results suggest that carnitine and acetylcarnitine could affect the differentiation rate of adult stem cells by regulating mitochondrial metabolism. The effects of these two small molecules give rise to the possibility of employing such metabolites in tissue-engineering systems to enhance cell differentiation and tissue development.

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“…However, expression of SLC27A2 was not increased in the rat ileum after 10 days of exercise [8]. SLC27A1 has been proven to be related to the tricarboxylic acid cycle activity and mitochondrial function [24]. Furthermore, SLC27A1 is a component of lipid metabolism pathways [26].…”

Section: Slc27a1 Slc27a2mentioning

confidence: 99%

Critical Genes in White Adipose Tissue Based on Gene Expression Profile Following Exercise

2018

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Exercise is recognized as an effective method to prevent obesity and alleviate metabolic diseases. Browning of white adipose has the advantage of decreasing insulin resistance. We aim to identify critical differentially expressed genes (DEGs) in white adipose tissue after exercise. We downloaded the gene dataset GSE68161 of C57BL/6 mice from the Gene Expression Omnibus (GEO) database. Then, we analyzed the effect of exercise on up-regulated and down-regulated DEGs by GEO2R and performed protein-protein interaction network analyses. We then identified hub-genes in white adipose tissue and crosstalk genes of a single pathway by the STRING database and Cytoscape. In this study, 72 DEGs were screened out, and they mainly function in glycerol-3-phosphate dehydrogenase activity and in the primary biological process of fatty acid oxidation regulation. The top 5 hub-genes screened out were SLC27A1, COX7A1, PPARGC1A, FABP3, and UCP1. The 3 crosstalk genes found were SLC27A1, SLC27A2, and PPARA. These 3 genes might function as a bridge of the PPAR signaling pathway, adipocytokine signaling pathway and the insulin resistance pathway. SLC27A1 is critical gene for the interactions of signaling pathways in subcutaneous white adipose tissue. Therefore, further relationships between the browning of white adipose and insulin resistance need to be studied.

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A novel role for fatty acid transport protein 1 in the regulation of tricarboxylic acid cycle and mitochondrial function in 3T3-L1 adipocytes

Cited by 26 publications

References 60 publications

Cell‐Autonomous Brown‐Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice

Cell‐Autonomous Brown‐Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice

Carnitine and acetylcarnitine modulate mesenchymal differentiation of adult stem cells

Critical Genes in White Adipose Tissue Based on Gene Expression Profile Following Exercise

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