A Novel Role of Ascorbic Acid in Anti-Inflammatory Pathway and ROS Generation in HEMA Treated Dental Pulp Stem Cells

Diomede, Francesca; Marconi, Guya Diletta; Guarnieri, Simone; D’Attilio, Michele; Cavalcanti, Marcos Fernando Xisto Braga; Mariggiò, Maria Addolorata; Pizzicannella, Jacopo; Trubiani, Oriana

doi:10.3390/ma13010130

Cited by 46 publications

(32 citation statements)

References 77 publications

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“…In agreement with previous studies [ 53 , 54 , 55 ], the data obtained in the present work demonstrated that treatment with AA showed a reduction in the expression levels of the NLP3 inflammasome that led to a reduction in the production of IL-1β through the TLR4/MyD88/NFκB signaling pathway in the hGMSCs and e-hGMSCs. Moreover, our findings evidenced a protective role played by AA through the modulation of TLR4/MyD88/NFκB/NLRP3/Caspase-1/IL-1β, reestablishing the microenvironment before LPS-G stimulation.…”

Section: Discussionsupporting

confidence: 93%

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

et al. 2021

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Human gingival mesenchymal stem cells (hGMSCs) and endothelial committed hGMSCs (e-hGMSCs) have considerable potential to serve as an in vitro model to replicate the inflammation sustained by Porphyromonas gingivalis in periodontal and cardiovascular diseases. The present study aimed to investigate the effect of ascorbic acid (AA) on the inflammatory reverting action of lipopolysaccharide (LPS-G) on the cell metabolic activity, inflammation pathway and reactive oxygen species (ROS) generation in hGMSCs and e-hGMSCs. Cells were treated with LPS-G (5 μg mL−1) or AA (50 μg mL−1) and analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, immunofluorescence and Western blot methods. The rate of cell metabolic activity was decreased significantly in LPS-G-treated groups, while groups co-treated with LPS-G and AA showed a logarithmic cell metabolic activity rate similar to untreated cells. AA treatment attenuated the inflammatory effect of LPS-G by reducing the expression of TLR4/MyD88/NFκB/NLRP3/Caspase-1/IL-1β, as demonstrated by Western blot analysis and immunofluorescence acquisition. LPS-G-induced cells displayed an increase in ROS production, while AA co-treated cells showed a protective effect. In summary, our work suggests that AA attenuated LPS-G-mediated inflammation and ROS generation in hGMSCs and e-hGMSCs via suppressing the NFκB/Caspase-1/IL-1β pathway. These findings indicate that AA may be considered as a potential factor involved in the modulation of the inflammatory pathway triggered by LPS-G in an vitro cellular model.

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Section: Discussionsupporting

confidence: 93%

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

et al. 2021

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“…Ascorbic acid also has potent anti-inflammatory properties (Diomede et al, 2020), therefore; positively influencing host-defence mechanism towards tissues repair during infection (Goldschmidt, 1991). Its antiscorbutic properties play a pivotal role in the synthesis of collagen through its ability to act as an electron-donor maintaining the ferrous in collagen hydroxylases in an active state (Du et al, 2012).…”

Section: Ascorbic Acidmentioning

confidence: 99%

What the future holds for regenerative endodontics: novel antimicrobials and regenerative strategies

Matoug‐Elwerfelli¹,

Nazzal²,

Duggal³

et al. 2021

eCM

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Regenerative/revitalisation endodontic techniques are increasingly used as a treatment approach for the management of immature permanent teeth with necrotic pulps. Different chemical irrigants and medicaments are routinely used clinically for intra-canal disinfection. However, despite remarkable progress in this field, coronal discolouration, cell cytotoxicity, difficulty of removal of organic biofilm from the root canal, development of sensitisation and antimicrobial resistance are still challenges to this line of treatment. This review critically discusses and challenges the current status quo of antimicrobials used in regenerative endodontics and sheds the light on future alternative antimicrobial materials with regenerative potential.

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“…gingivalis , G), that afflicts a large number of people [ 16 , 17 ]. Ascorbic acid (AA), known as vitamin C or ascorbate, consumed as a dietary supplement is a water-soluble vitamin that helps many enzyme activities [ 18 ]. AA is deeply involved in the reduction of reactive oxygen species (ROS) and showed a key role as an antisenescence agent [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid: A New Player of Epigenetic Regulation in LPS‐gingivalis Treated Human Periodontal Ligament Stem Cells

Marconi

Fonticoli

Guarnieri

et al. 2021

Oxidative Medicine and Cellular Longevity

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Periodontitis is usually sustained from microorganism of oral cavity, like Porphyromonas gingivalis (P. gingivalis). Periodontal disease is an infectious disease that afflicts a large number of people. Researches are investigating on the mesenchymal stem cells (MSCs) response to inflammatory events in combination with antioxidant substances. In particular, ascorbic acid (AA) increased cell proliferation, upregulated the cells pluripotency marker expression, provide a protection from inflammation, and induced the regeneration of periodontal ligament tissue. The purpose of the present research was to investigate the effects of AA in primary culture of human periodontal ligament stem cells (hPDLSCs) exposed to P. gingivalis lipopolysaccharide (LPS-G). The effect of AA on hPDLSCs exposed to LPS-G was determined through the cell proliferation assay. The molecules involved in the inflammatory pathway and epigenetic regulation have been identified using immunofluorescence and Western blot analyses. miR-210 level was quantified by qRT-PCR, and the ROS generation was finally studied. Cells co-treated with LPS-G and AA showed a restoration in terms of cell proliferation. The expression of NFκB, MyD88, and p300 was upregulated in LPS-G exposed cells, while the expression was attenuated in the co-treatment with AA. DNMT1 expression is attenuated in the cells exposed to the inflammatory stimulus. The level of miR-210 was reduced in stimulated cells, while the expression was evident in the hPDLSCs co-treated with LPS-G and AA. In conclusion, the AA could enhance a protective effect in in vitro periodontitis model, downregulating the inflammatory pathway and ROS generation and modulating the miR-210 level.

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A Novel Role of Ascorbic Acid in Anti-Inflammatory Pathway and ROS Generation in HEMA Treated Dental Pulp Stem Cells

Cited by 46 publications

References 77 publications

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

Antioxidant Ascorbic Acid Modulates NLRP3 Inflammasome in LPS-G Treated Oral Stem Cells through NFκB/Caspase-1/IL-1β Pathway

What the future holds for regenerative endodontics: novel antimicrobials and regenerative strategies

Ascorbic Acid: A New Player of Epigenetic Regulation in LPS‐gingivalis Treated Human Periodontal Ligament Stem Cells

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