A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma

Ahmed, Sameh; Mahmoud, Ashraf M.

doi:10.1016/j.talanta.2015.07.007

Cited by 35 publications

(15 citation statements)

References 35 publications

(60 reference statements)

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“…Vitamins D 3 and K deficiencies occur due to induction of Cyp450 and eradication of bacteria (responsible for synthesis of vitamin K), respectively [ 6 – 8 ]. Many scientific papers described the analysis of vitamin D 3 and MK-7 in various matrixes such as biological fluids, foods, plant material by fluorimetric, UV-Vis, and MS detection after chromatographic separation [ 9 – 14 ]. Determination of vitamin K is also reported by HPLC coupled to chemiluminescence and electrochemical detectors [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

Jehangir

Ahmed

Shafiq

et al. 2017

Journal of Analytical Methods in Chemistry

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A newly developed method based on ultrahigh performance liquid chromatography (UHPLC) was optimized for the simultaneous determination of vitamin D3 and menaquinone-7 (MK-7) in tablet formulation in the present study. UHPLC separation of vitamin D3 and MK-7 was performed with ACE Excel 2 C18-PFP column (2 μm, 2.1 × 100 mm) at 0.6 mL min−1 flow rate, whereas the mobile phase consisted of methanol/water (19 : 1, v/v, phase A) and isopropyl alcohol (99.9%, phase B) containing 0.5% triethylamine. Isocratic separation of both the analytes was performed at 40°C by pumping the mobile phases A and B in the ratio of 50 : 50 (v/v, pH, 6.0). Both analytes were detected at a wavelength of 265 nm and the injection volume was 1.0 μL. The overall runtime per sample was 4.5 min with retention time of 1.26 and 3.64 min for vitamin D3 and MK-7, respectively. The calibration curve was linear from 5.0 to 100 μg mL−1 for vitamin D3 and MK-7 with a coefficient of determination (R2) ≥ 0.9981, while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.46 and 2.21%, respectively. The proposed HPLC method was demonstrated to be simple and rapid for the determination of vitamin D3 and MK-7 in tablets.

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Section: Introductionmentioning

confidence: 99%

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

Jehangir

Ahmed

Shafiq

et al. 2017

Journal of Analytical Methods in Chemistry

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“…Protein precipitation (PP), liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are the three most common techniques used for vitamin K purification process in biological matrix to achieve efficient analyte recovery by reducing ion suppression and enhancement of analyte signal. Other approaches such as performing derivatization reactions (25)(26)(27)(28), emulsification mircroextraction (29), and sonication (30) have also been reported in sample preparations. PP is a rapid, generic and relative inexpensive sample preparation method.…”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

A concise review of quantification methods for determination of vitamin K in various biological matrices

Zhang

Bala

Mao

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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Vitamin K is an essential nutrient in the body and involved in numerous physiological and pathophysiological functions. Both the lack and surplus of vitamin K can put human health at risk. Therefore, it becomes necessary to monitor vitamin K concentrations in different biomatrices through establishing sensitive and specific analytical methods. This review collectively describes an updated overview of the sample pretreatment methodologies and methods for quantitative determination of vitamin K that have been used in last two decades. High Performance Liquid Chromatography (HPLC) is commonly utilized as a standard for separation of vitamin K in combination with different detection including spectroscopic, spectrometric, fluorometric and mass spectroscopy. Recent progress in sample pretreatment technologies and quantitation methodologies have enhanced the ability to identify and quantitate vitamin K in biomatrices to further advance our understanding of the role of this vitamin in human health and disease.

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“…The method is simple, fast, cheap and environmentally friendly and its extract is also compatible with HPLC/LC, CE and/or GC instruments [36,37]. SALLE has been applied for extraction of various organic compounds including α-dicarbonyl compounds in beer [24] and wines [25]; biogenic amines in fruit juices and alcoholic beverages [26]; N-nitrosamines in skin care cosmetics [27]; trimetazidine in rat plasma [28]; methoxetamine in rat tissues (rat brain, liver and lungs) [29]; sulfonamides from different matrices (tea, water, milk, honey, human urine, plasma and blood) [30,31]; temozolomide from plasma [32]; vitamin K homologues in human plasma [33]; multi-mycotoxin biomarkers in pig urine [34]; betalactam antibiotics in infant dairy products [35]; benzimidazole fungicides [36]; sulfonylurea herbicides in water and banana juice samples [37]; triazole pesticides in water samples [38]; multiresidue pesticides in surface, ground and drinking water [39]; and chlorophenols in wine [40]. However, to date there is no report on the use of SALLE procedure in combination with HPLC-UV/Vis for analysis of multiclass pesticide residues in alcoholic beverages.…”

Section: Introductionmentioning

confidence: 99%

Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

Bedassa¹

2017

SJAC

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Abstract:A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r 2 ) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5-12.8 µg L -1 , respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.

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A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma

Cited by 35 publications

References 35 publications

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

A concise review of quantification methods for determination of vitamin K in various biological matrices

Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

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A novel salting-out assisted extraction coupled with HPLC- fluorescence detection for trace determination of vitamin K homologues in human plasma

Cited by 35 publications

References 35 publications

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D3and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D3and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

A concise review of quantification methods for determination of vitamin K in various biological matrices

Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

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UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation