A Novel SDS Rinse and Immunomagnetic Beads Separation Combined with Real-Time Loop-Mediated Isothermal Amplification for Rapid and Sensitive Detection of Salmonella in Ready-to-Eat Duck Meat

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

“…Bi et al. (2020) also reported that, under the optimum conditions, the CE value was over 98% when the IMPs were used to capture Salmonella cells in the range of 10 1 to 10 5 CFU/mL. Additionally, Li et al.…”

“…Similarly, Cai, Wang, Zheng, and Lin (2018) found that the CE value for S. typhimurium in PBS with the concentration of 10 CFU/mL was 65%, while that for 10 2 to 10 4 CFU/mL was about 80%. Bi et al (2020) also reported that, under the optimum conditions, the CE value was over 98% when the IMPs were used to capture Salmonella cells in the range of 10 1 to 10 5 CFU/mL. Additionally, evaluated the influence of the cell concentration (10 1 to 10 6 CFU/mL) on the separation of L. monocytogenes.…”

“…In addition, the mass ratio of antibodies to MPs (1:40, 1:20, 1:10, and 1:5) was tested to optimize the IMS method for Salmonella . The result displayed that the CE continued to increase with the increase of the molar ratio of antibodies to MPs and reached its maximum of 99.3% at 1:5 (Bi et al., 2020). For the principle and procedure of IMPs preparation, and the properties of immobilized antibody and obtained IMPs are different, the optimization of antibody amount is necessary.…”

“…In recent years, a growing number of studies have focused on enhancing the detection sensitivity and reducing the testing time from days to hours. The representative methods include immunology‐based methods (enzyme linked immunosorbent assay, ELISA; immunochromatographic assay, ICA), nucleic acid‐based methods (polymerase chain reaction, PCR; loop‐mediated isothermal amplification, LAMP), signal‐based methods (such as fluorochrome and quantum dots [QDs]), and so on (Bi et al., 2020; Li et al., 2017; Li et al., 2019; Park, Park, Ok, Chang, & Lim, 2020; Wei et al., 2019; Xue, Zheng, Zhang, Jin, & Lin, 2018). However, food samples are usually complex and heterogeneous systems.…”

“…On the basis of the specific recognition of antibody and characteristics of magnetic features, the target bacteria can be efficiently separated from the food system through IMS, which is useful for purifying sample system and removing interference (Kim, Park, Kim, & Cho, 2014). The IMS pretreatment can improve the concentration of target bacteria, thereby reducing analysis time and improving detection sensitivity (Bi et al., 2020). Owning to the above advantages, IMS has been used in the selective isolation and enrichment of foodborne pathogens and spoilage microorganisms from a range of food matrices (Lim et al., 2017; Yan et al., 2016).…”