A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography

Abstract

Determination of sialic acids utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to HPLC.

Chemical Tagging in Mass Spectrometry for Systems Biology

“…Sialic acid groups on glycans are labile in MS/MS experiments and therefore need to be stabilized through chemical derivatization . These carboxylate stabilizing reactions are often amidations.…”

“…Sialic acid groups on glycans are labile in MS/MS experiments and therefore need to be stabilized through chemical derivatization. 93 These carboxylate stabilizing reactions are often amidations. Depending on the glycan orientation, the carbodiimide coupling can result in either an amidation if the carboxylate is freely rotating, as in α2,6 linked sialic acids, or ester/lactone formation between the carboxylate and the neighboring hydroxyl group, as in the case of α2,3linked sialic acids (Figure 6).…”

Increased serum sialic acid is associated with morbidity and mortality in a murine model of dengue disease

“…parental glycoconjugates in mouse serum [32]. This approach allowed us to monitor multiple ions (three ions) during analysis to achieve enhanced specificity for Sia identification and quantitation in the presence of complex biological matrices such as serum.…”

Determination of sialic acid in serum samples by dispersive solid-phase extraction based on boronate-affinity magnetic hollow molecularly imprinted polymer sorbent

“…The serum is the most important human biouid containing SA and especially valuable in clinical diagnosis of several diseases. 15 Gruszewska et al 16 have described the marker capability of SA in serum for diagnosis and evaluation the location of tumor in patients with primary pancreatic cancer. So the measurement of serum SA can be valuable in earlier diagnosis of malignant disease or monitoring the tumour bulk in response to treatment.…”