A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

Lim, Lih Shan; Chin, Suk Fun; Pang, Suh Cem; Sum, Magdline Sia Henry; Perera, David

doi:10.17576/jsm-2017-4612-21

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…Under optimum conditions, namely 20 minutes of incubation time and 50 µg/mL JEV antibody was used, Ag NPs-modified SPCE biosensor strips exhibited LOD of 2.60 ng/mL to JEV antigen, with an analysis assay time of 20 min. The LOD of 2.60 ng/mL to JEV antigen of this work is much lower as compared to the Ag NPs sensing probe with LOD of 12.8 ng/mL reported previously [12].…”

Section: Discussioncontrasting

confidence: 60%

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Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

Chin¹,

Lim²,

Lai³

et al. 2019

Nano BioMed ENG

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In this study, we aim to fabricate a silver nanoparticles (Ag NPs) based electrochemical biosensor for Japanese Encephalitis virus antigen detection. Ag NPs were deposited onto screen-printed carbon electrode (SPCE) and the electrochemical properties of the Ag NPs modified SPCE were investigated via electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was observed that the deposition of Ag NPs onto electrode surface has significantly enhanced the conductivity up to 20.5% than that of bare SPCE. The EIS data indicated limit of detection (LOD) of 2.60 ng/mL (at S/ N = 3) towards JEV antigen, with an analysis assay time of 20 min. This presented Ag NPs modified SPCE has demonstrated a promising and rapid alternative to conventional biosensing techniques towards JEV antigen.

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Section: Discussioncontrasting

confidence: 60%

“…Ag NPs exhibited excellent conductivity property and is used to enhance the performance of a glucose biosensor [11]. Our research group has attempted to fabricate an optical JEV biosensor by coating of silver nanoparticles onto glass substrates [12].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

Chin¹,

Lim²,

Lai³

et al. 2019

Nano BioMed ENG

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“…As a noteworthy example to illustrate this concept, Vinayagam et al reported the use of triangular AgNPs (TAg) that were oligomerized with a ssDNA probe, designed complementary to the target RNA sequence of a specific Dengue virus strain, that could distinguish between different serotypes of dengue virus strains [147] . Parallelly, DNA tetrahedrons crosslinked with AgNP probes for the detection of HIV virus [148] and sandwich based AgNP optical immunosensors for the detection of Japanese encephalitis Virus (JEV) antigens have been fabricated as well, by the immobilization of JEV antibodies on AgNPs that were deposited on amine functionalized glass sides [149] …”

Section: Agnps In Clinical Diagnosis Of Viral Infectionsmentioning

confidence: 99%

The Emerging Roles of Silver Nanoparticles to Target Viral Life Cycle and Detect Viral Pathogens

Ghosh

Ahammed

Mishra

et al. 2022

Chemistry An Asian Journal

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Along the line of recent vaccine advancements, new antiviral therapeutics are compelling to combat viral infection-related public health crises. Several properties of silver nanoparticles (AgNPs) such as low level of cytotoxicity, ease of tunability of the AgNPs in the ultra-small nanoscale size and shape through different convenient bottom-up chemistry approaches, high penetration of the composite with drug formulations into host cells has made AgNPs, a promising candidate for developing antivirals. In this review, we have highlighted the recent advancements in the AgNPs based nano-formulations to target cellular mechanisms of viral propagation, immune modulation of the host, and the ability to synergistically enhance the activity of existing antiviral drugs. On the other hand, we have discussed the recent advancements on AgNPs based detection of viral pathogens from clinical samples using inherent physicochemical properties. This article will provide an overview of our current knowledge on AgNPs based formulations that has promising potential for developing a counteractive strategy against emerging and existing viruses.

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“…Exploiting the surface plasmon resonance (SPR) property of silver nanoparticles (AgNPs), an optical based sensing probe was fabricated with synthesised AgNPs drop casted onto (3-Aminopropyl)triethoxysilane (APTES) amine functionalized glass immobilised with JEV antibodies. Changes in the absorbance of light was detected upon binding of target JEV antigen (Ag) and analysed which resulted in a detection limit of 12.8 ng/ml (Lim et al 2017 ). Similarly, an optical Lateral Flow Assay (LFA) was developed for detecting JEV antibodies (Ab) in swine serum for sero-surveillance and mass screening using the SPR properties of AuNPs.…”

Section: Biosensors For Jev Diagnosticsmentioning

confidence: 99%

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

Roberts

Gandhi

2022

Proc.Indian Natl. Sci. Acad.

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Advanced biosensor technology research is imperative for the management of infectious disease outbreaks such as Japanese Encephalitis (JE), a zoonotic disease caused by the flavivirus JE virus (JEV) which is transmitted to humans (dead-end hosts) from the amplification host, pigs, via mosquitoes. To avoid future pandemic scenarios, proactive research rather than responsive research in the field of diagnostics is a requirement for development of rapid, sensitive and specific screening detection methods. In this mini-review, we have critically compared and evaluated the different types of biomarkers (antigen, antibody, nucleic acid) identified for JEV diagnostics and their specific roles in the manifestation of the infection which may be potentially used for therapeutics and drug development as no treatment is available for JE. Furthermore, different biosensors developed for the detection of JEV biomarkers have been discussed in detail to give an overview of the working principles (electrochemical, optical, etc.), fabrication components (signal amplifier, bioreceptor, etc.), detection limits and response times. This review provides a compact compiled base on available JEV diagnostic research work being currently carried out along with their limitations, future prospective, and major challenges faced. This will enable future development of rapid point-of-care diagnostic screening methods for JEV infection management, which may help reduce number of fatalities.

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A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

Abstract: A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV

Cited by 13 publications

References 29 publications

Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

Electrochemical Determination of Japanese Encephalitis Virus Antigen Using Silver Nanoparticles Modified Screen-Printed Carbon Electrode

The Emerging Roles of Silver Nanoparticles to Target Viral Life Cycle and Detect Viral Pathogens

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

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