A novel, simplified and stability-indicating high-throughput ultra-fast liquid chromatography method for the determination of rosmarinic acid in nanoemulsions, porcine skin and nasal mucosa

Fachel, Flávia Nathiely Silveira; Nemitz, Marina Cardoso; Medeiros-Neves, Bruna; Veras, Kleyton Santos; Bassani, Valquíria Linck; Koester, Letícia Scherer; Henriques, Amélia Teresinha; Teixeira, Hélder Ferreira

doi:10.1016/j.jchromb.2018.03.020

Cited by 22 publications

(11 citation statements)

References 38 publications

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“…Owing to its importance in this field, achieving a fast, selective, and sensitive method for RA and PCA determination is necessary. Capillary electrophoresis , flow injection , liquid chromatography‐mass spectrometry , and ultrafast liquid chromatography techniques are commonly used for the detection of PCA or RA. However, they have time‐consuming sample preparation steps, are expensive to operate, and consume many hazardous solvents.…”

Section: Introductionmentioning

confidence: 92%

Simultaneous Determination of Rosmarinic Acid and Protocatechuic Acid at Poly(o‐Phenylenediamine)/Pt Nanoparticles Modified Glassy Carbon Electrode

Özdokur

Koçak

2019

Electroanalysis

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A system of Pt nanoparticles and poly(ortho‐phenylenediamine) film electrochemically deposited onto a glassy carbon electrode (GCE/PoPD/Pt) was fabricated. Scanning electron microscopy, Fourier‐transform infrared spectroscopy, and atomic force microscopy techniques were used to identify the surface characteristics of the composite electrode. The conductive polymers and Pt nanoparticles together resulted in a synergistic effect, and the new formed surface was highly active against polyphenolic structures. Rosmarinic acid (RA) and protocatechuic acid (PCA) are phenolic compounds found in plants, and they are used in many applications, particularly as pharmaceuticals. The GCE/PoPD/Pt was used for the simultaneous determination of RA and PCA in a pH 2.0 H2SO4 solution for the first time. The RA and PCA concentrations were determined using differential pulse voltammetry (DPV) and chronoamperometry. By the amperometry measurement, for RA and PCA, a linear relation was observed in the concentration ranges of 1–55 μmol L−1 and 1–60 μmol L−1, with detection limits of 0.5 μmol L−1 and 0.6 μmol L−1, respectively. In the simultaneous determination with DPV, the detection limits for both RA and PCA were calculated as 0.7 μmol L−1. The GCE/PoPD/Pt was successfully used for the simultaneous determination of RA and PCA in a real sample, and its accuracy was verified by high‐performance liquid chromatography studies.

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Section: Introductionmentioning

confidence: 92%

Simultaneous Determination of Rosmarinic Acid and Protocatechuic Acid at Poly(o‐Phenylenediamine)/Pt Nanoparticles Modified Glassy Carbon Electrode

Özdokur

Koçak

2019

Electroanalysis

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“…Nevertheless, RA presents some drawbacks that can be relevant causes for the product's loss of effectiveness, such as poor water solubility, high instability and poor permeability through biological barriers . For this reason, technological approaches to circumvent these problems have been studied, such as the use of lipid‐nanotechnology‐based delivery systems . Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA.…”

Section: Introductionmentioning

confidence: 99%

“…Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA. Nanoemulsions are dispersions of two immiscible liquids (oil‐in‐water) stabilized by an appropriate surfactant system, that have been widely studied as a promising alternative for the treatment of skin diseases and for cosmetics purposes, since their small droplet sizes can improve active lipophilic molecules penetration through the skin, increasing their topical effect . Nevertheless, for topical application, the low viscosity from nanoemulsions is a concern and the design of a semisolid formulation is a key consideration.…”

Section: Introductionmentioning

confidence: 99%

“…[5,8] For this reason, technological approaches to circumvent these problems have been studied, such as the use of lipid-nanotechnologybased delivery systems. [18][19][20][21][22] Our research group has recently been interested in the development of topical nanoemulsions as delivery systems for RA. Nanoemulsions are dispersions of two immiscible liquids (oil-in-water) stabilized by an appropriate surfactant system, that have been widely studied as a promising alternative for the treatment of skin diseases and for cosmetics purposes, since their small droplet sizes can improve active lipophilic molecules penetration through the skin, increasing their topical effect.…”

Section: Introductionmentioning

confidence: 99%

“…Nanoemulsions are dispersions of two immiscible liquids (oil-in-water) stabilized by an appropriate surfactant system, that have been widely studied as a promising alternative for the treatment of skin diseases and for cosmetics purposes, since their small droplet sizes can improve active lipophilic molecules penetration through the skin, increasing their topical effect. [18,20,23,24] Nevertheless, for topical application, the low viscosity from nanoemulsions is a concern and the design of a semisolid formulation is a key consideration. In the literature, only one study described the development of semisolid formulations containing Melissa officinalis L. extract for topical application and did not use nanotechnology-based products for the improvement of RA skin retention/permeation.…”

Section: Introductionmentioning

confidence: 99%

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Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

Marafon

Fachel

Prá

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives Natural sources with antioxidant activity, such as rosmarinic acid (RA), have been considered as an interesting approach for the development of new anti‐ageing skin products. In this context, this study aimed to develop hydrogels containing RA‐loaded nanoemulsions and evaluate the effect of the addition of Tween® 80 (a nonionic cosurfactant) in formulations intended to be used for topical application. Methods Physico‐chemical characterization, in‐vitro release and skin retention/permeation from hydrogels of RA‐loaded nanoemulsions (containing or not Tween® 80) were evaluated. The RA‐loaded nanoemulsion safety profiles were also investigated in keratinocytes (HaCaT cells). Key findings It was observed that all formulations presented adequate physico‐chemical characterization for topical application. Furthermore, the results also demonstrated that the presence of Tween® 80 decreased the droplet size and polydispersity index of nanoemulsions and hydrogels. An extended RA release was noted for the hydrogels. However, when comparing the hydrogels, a positive effect of the presence of Tween® 80 on RA retention/permeation in total skin was seen. The RA‐loaded nanoemulsion safety profiles demonstrated a good tolerability (3.125–100 μm) in HaCaT cells. Conclusions The overall results demonstrated that the formulations developed in this study can be considered as a suitable carrier for RA in a topical application targeting new anti‐ageing skin care products.

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A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Balestrin

Fachel

Koester

et al. 2020

Phytochemical Analysis

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Introduction: Achyrocline satureioides (marcela or macela) is a plant widely used in folk medicine in South America. Recently, there has been increasing interest for the development of skin care products containing A. satureoides extracts, due to its welldocumented antioxidant, antiherpetic, and wound healing properties. Objectives: The present study aimed to develop and validate a yet unexplored stability-indicating and robust ultra-fast liquid chromatography (UFLC) method for the simultaneous quantification of the main flavonoids of A. satureioides in extracts, nanoemulsions, and porcine skin layers. Material and methods: The chromatographic separation of flavonoids quercetin, luteolin, and 3-O-methylquercetin was performed on a Luna C18 analytical column (100 mm × 2.0 mm i.d.; particle size 2.5 μm) using isocratic elution with methanol/phosphoric acid 1% (48:52 v/v) with a flow rate of 0.3 mL/min at 40 C. Results: The method was found to be specific, linear (R > 0.998), precise, accurate, and robust for all flavonoids assayed in A. satureioides extract, nanoemulsions, and porcine ear skin. A low matrix effect was noted for all complex matrices. The stability-indicating UFLC method was evaluated by submitting isolated flavonoids, a mixture of standards, and A. satureioides extract to acidic, alkaline, oxidative, UV-A/UV-C light, and thermal stress conditions. No peaks were found co-eluting with the flavonoids of interest in all matrices. The robustness of the method was confirmed using Plackett-Burman experimental design. Conclusion: The short run time (8 min) and reliability of the method could be useful for the determination of A. satureioides flavonoids in topical product development since extracts of this medicinal plant have been used to treat various skin disorders.

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A novel, simplified and stability-indicating high-throughput ultra-fast liquid chromatography method for the determination of rosmarinic acid in nanoemulsions, porcine skin and nasal mucosa

Cited by 22 publications

References 38 publications

Simultaneous Determination of Rosmarinic Acid and Protocatechuic Acid at Poly(o‐Phenylenediamine)/Pt Nanoparticles Modified Glassy Carbon Electrode

Simultaneous Determination of Rosmarinic Acid and Protocatechuic Acid at Poly(o‐Phenylenediamine)/Pt Nanoparticles Modified Glassy Carbon Electrode

Development, physico-chemical characterization and in-vitro studies of hydrogels containing rosmarinic acid-loaded nanoemulsion for topical application

A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

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