A Novel Single-Nucleotide Mutation in a CLAVATA3 Gene Homolog Controls a Multilocular Silique Trait in Brassica rapa L.

Fan, Chuchuan; Wu, Yudi; Yang, Qingyong; Yang, Yang; Meng, Qingwei; Zhang, Keqiao; Li, Jinguo; Wang, Jinfang; Zhou, Yongming

doi:10.1093/mp/ssu090

Cited by 51 publications

(69 citation statements)

References 12 publications

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“…All clv1 mutants in Arabidopsis exhibited weak, intermediate or strong multilocular phenotypes 27 , and previous studies had shown that most Brassicas plants with multiloculus had variable valve numbers in the siliques of the same plant 10, 11 . However, in this study, J163-4 planted in both central and northwestern China exhibited four valves stably in trilocular siliques.…”

Section: Discussionmentioning

confidence: 75%

“…Moreover, all the clv1 mutants with intermediate and strong multilocular phenotypes were dominant negative, and the mutant of CLV1 homologous gene in tomato was also expected to be dominant-negative 16, 27 . Similarly, most of the mutants with multiloculus discovered in Brassicas showed variable valve numbers 10, 11 . However, it has been unknown whether the molecular mechanism controlling multiloculus is similar between Arabidopsis and Brassicas or the dominant-negative character is associated with the instability of multilocular silique trait.…”

Section: Introductionmentioning

confidence: 92%

“…rapa was found to be controlled by a CLAVATA3 ( CLV3 ) gene homologue, and it suggested that the feedback loop involving CLV3 and WUSCHEL ( WUS ) played a major role in carpel development 11 . Multilocular trait was also observed and genetically investigated in Arabidopsis 12–15 and tomato 16 .…”

Section: Introductionmentioning

confidence: 99%

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Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Cao

et al. 2017

Sci Rep

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As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype. Phylogenetic analysis showed that Mc1 genes were conserved and widespread in land plants. Two amino acid sites had undergone positive selection in the ancestor of Mc1 genes, and then purifying selection was the dominant force after the divergence of dicots and monocots from their common ancestor in the evolutionary process, indicating that Mc1 genes are conserved in modern land plants. Our results provided new insights in molecular regulatory mechanism of multilocularity in rapeseed, and better understanding of molecular mechanism in crop yield improvement.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 92%

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Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Cao

et al. 2017

Sci Rep

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“…We have shown that B. rapa harbouring loss‐of‐function clv3 alleles produces more locules and higher seed yield (Fan et al ., ). Thus, modifications of CLV3 in B. napus may provide an opportunity to breed high‐yield varieties.…”

Section: Resultsmentioning

confidence: 97%

Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development

Yang

Zhu

et al. 2018

Plant Biotechnology Journal

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143

118

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SummaryMultilocular silique is a desirable agricultural trait with great potential for the development of high‐yield varieties of Brassica. To date, no spontaneous or induced multilocular mutants have been reported in Brassica napus, which likely reflects its allotetraploid nature and the extremely low probability of the simultaneous random mutagenesis of multiple gene copies with functional redundancy. Here, we present evidence for the efficient knockout of rapeseed homologues of CLAVATA3 (CLV3) for a secreted peptide and its related receptors CLV1 and CLV2 in the CLV signalling pathway using the CRISPR/Cas9 system and achieved stable transmission of the mutations across three generations. Each BnCLV gene has two copies located in two subgenomes. The multilocular phenotype can be recovered only in knockout mutations of both copies of each BnCLV gene, illustrating that the simultaneous alteration of multiple gene copies by CRISPR/Cas9 mutagenesis has great potential in generating agronomically important mutations in rapeseed. The mutagenesis efficiency varied widely from 0% to 48.65% in T0 with different single‐guide RNAs (sgRNAs), indicating that the appropriate selection of the sgRNA is important for effectively generating indels in rapeseed. The double mutation of BnCLV3 produced more leaves and multilocular siliques with a significantly higher number of seeds per silique and a higher seed weight than the wild‐type and single mutant plants, potentially contributing to increased seed production. We also assessed the efficiency of the horizontal transfer of Cas9/gRNA cassettes by pollination. Our findings reveal the potential for plant breeding strategies to improve yield traits in currently cultivated rapeseed varieties.

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“…SlWUS is a candidate for the lc QTL, and fas was recently found to be caused by a genomic rearrangement that alters the expression of SlCLV3 (Muños et al, 2011;Xu et al, 2015). Similarly, a naturally occurring CLV3 mutation in mustard (Brassica rapa) corresponds to the Multilocular (more than two carpels) locus, which increases seed production (Fan et al, 2014). These diverse examples indicate that CLV-WUS genes have been selected in diverse crops during domestication, and could provide further crop yield increases -for example, by engineering weak alleles using CRISPR.…”

Section: Potential Applications In Agriculturementioning

confidence: 99%

CLAVATA-WUSCHEL signaling in the shoot meristem

2016

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Shoot meristems are maintained by pluripotent stem cells that are controlled by CLAVATA-WUSCHEL feedback signaling. This pathway, which coordinates stem cell proliferation with differentiation, was first identified in Arabidopsis, but appears to be conserved in diverse higher plant species. In this Review, we highlight the commonalities and differences between CLAVATA-WUSCHEL pathways in different species, with an emphasis on Arabidopsis, maize, rice and tomato. We focus on stem cell control in shoot meristems, but also briefly discuss the role of these signaling components in root meristems.

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A Novel Single-Nucleotide Mutation in a CLAVATA3 Gene Homolog Controls a Multilocular Silique Trait in Brassica rapa L.

Abstract: Analogs of the CLV3 peptide: synthesis and structure-activity relationships focused on proline residues. Plant Cell Physiol. 52, 30-36.

Cited by 51 publications

References 12 publications

Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development

CLAVATA-WUSCHEL signaling in the shoot meristem

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