A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase (
            <i>msrA</i>
            ) Expression for Oxidative Stress Adaptation

Chen, Yun; Zhao, Mingming; Lin, Min; Wang, Jin; Zuo, Kaijing

doi:10.1128/aem.00038-22

Cited by 6 publications

(2 citation statements)

References 59 publications

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“…D. radiodurans R1 organisms were collected under various abiotic stress conditions using the ambion ® RNA extraction kit from Life. Referring to the reported method [ 62 ], 16S was used as the internal reference gene, and a real-time quantitative polymerase chain reaction (qRT-PCR) was performed for dogH , dr0264 , dr1141 , dr1848 , treY , treZ and treS genes under different experimental conditions via the △△ Ct method. The relevant primers involved in this experiment are shown in Supplementary Table S2 and the qRT-PCR results are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

A Novel Glycoside Hydrolase DogH Utilizing Soluble Starch to Maltose Improve Osmotic Tolerance in Deinococcus radiodurans

Gui

Lin

Yan

et al. 2023

IJMS

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Deinococcus radiodurans is a microorganism that can adjust, survive or thrive in hostile conditions and has been described as “the strongest microorganism in the world”. The underlying mechanism behind the exceptional resistance of this robust bacterium still remains unclear. Osmotic stress, caused by abiotic stresses such as desiccation, salt stress, high temperatures and freezing, is one of the main stresses suffered by microorganisms, and it is also the basic response pathway by which organisms cope with environmental stress. In this study, a unique trehalose synthesis-related gene, dogH (Deinococcus radiodurans orphan glycosyl hydrolase-like family 10), which encodes a novel glycoside hydrolase, was excavated using a multi-omics combination method. The content accumulation of trehalose and its precursors under hypertonic conditions was quantified by HPLC-MS. Ours results showed that the dogH gene was strongly induced by sorbitol and desiccation stress in D. radiodurans. DogH glycoside hydrolase hydrolyzes α-1,4-glycosidic bonds by releasing maltose from starch in the regulation of soluble sugars, thereby increasing the concentration of TreS (trehalose synthase) pathway precursors and trehalose biomass. The maltose and alginate content in D. radiodurans amounted to 48 μg mg protein−1 and 45 μg mg protein−1, respectively, which were 9 and 28 times higher than those in E. coli, respectively. The accumulation of greater intracellular concentrations of osmoprotectants may be the true reason for the higher osmotic stress tolerance of D. radiodurans.

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Section: Methodsmentioning

confidence: 99%

A Novel Glycoside Hydrolase DogH Utilizing Soluble Starch to Maltose Improve Osmotic Tolerance in Deinococcus radiodurans

Gui

Lin

Yan

et al. 2023

IJMS

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“…The Δ drBON1 (Δ dr_0392 ) deletion mutant was constructed with double crossover recombination of a spectinomycin (spec) resistance cassette into the genome, as described previously [ 73 ]. PCR primers were designed based on the sequence of drBON1 in the genome.…”

Section: Methodsmentioning

confidence: 99%

Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions

Guo,

Zhan,

Zhang

et al. 2023

IJMS

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To grow in various harsh environments, extremophiles have developed extraordinary strategies such as biofilm formation, which is an extremely complex and progressive process. However, the genetic elements and exact mechanisms underlying extreme biofilm formation remain enigmatic. Here, we characterized the biofilm-forming ability of Deinococcus radiodurans in vitro under extreme environmental conditions and found that extremely high concentrations of NaCl or sorbitol could induce biofilm formation. Meantime, the survival ability of biofilm cells was superior to that of planktonic cells in different extreme conditions, such as hydrogen peroxide stress, sorbitol stress, and high UV radiation. Transcriptome profiles of D. radiodurans in four different biofilm development stages further revealed that only 13 matched genes, which are involved in environmental information processing, carbohydrate metabolism, or stress responses, share sequence homology with genes related to the biofilm formation of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Overall, 64% of the differentially expressed genes are functionally unknown, indicating the specificity of the regulatory network of D. radiodurans. The mutation of the drRRA gene encoding a response regulator strongly impaired biofilm formation ability, implying that DrRRA is an essential component of the biofilm formation of D. radiodurans. Furthermore, transcripts from both the wild type and the drRRA mutant were compared, showing that the expression of drBON1 (Deinococcus radioduransBON domain-containing protein 1) significantly decreased in the drRRA mutant during biofilm development. Further analysis revealed that the drBON1 mutant lacked the ability to form biofilm and DrRRA, and as a facilitator of biofilm formation, could directly stimulate the transcription of the biofilm-related gene drBON1. Overall, our work highlights a molecular mechanism mediated by the response regulator DrRRA for controlling extreme biofilm formation and thus provides guidance for future studies to investigate novel mechanisms that are used by D. radiodurans to adapt to extreme environments.

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The radioresistant and survival mechanisms of Deinococcus radiodurans

Liu

Zhang

2023

Radiation Medicine and Protection

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A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase ( msrA ) Expression for Oxidative Stress Adaptation

Abstract: The repair of oxidized proteins is an indispensable function allowing the extremophile D. radiodurans to grow in adverse environments. Msr proteins and various oxidoreductases can reduce oxidized Cys and Met amino acid residues of damaged proteins to recover protein function.

Cited by 6 publications

References 59 publications

A Novel Glycoside Hydrolase DogH Utilizing Soluble Starch to Maltose Improve Osmotic Tolerance in Deinococcus radiodurans

A Novel Glycoside Hydrolase DogH Utilizing Soluble Starch to Maltose Improve Osmotic Tolerance in Deinococcus radiodurans

Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions

The radioresistant and survival mechanisms of Deinococcus radiodurans

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