A novel strategy for rapid real-time chiral discrimination of enantiomers using serum albumin functionalized QCM biosensor

Su, Wen Cui; Zhang, Wei Guang; Sheng, Zhang; Fan, Jun; Yin, Xian‐Hong; Luo, Miao Li; Ng, Siu‐Choon

doi:10.1016/j.bios.2009.06.040

Cited by 44 publications

(33 citation statements)

References 24 publications

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“…The immobilization processes of SAs on QCM crystals were measured by a home‐made flow injection analysis (FIA) system, which was similar to that used in the reported literature . Chiral discrimination was investigated by using a home‐made QCM gas system described previously …”

Section: Methodsmentioning

confidence: 99%

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Chen

Zhang

et al. 2012

Chirality

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Quartz crystal microbalance (QCM) biosensor was used for the chiral recognition of five pairs of enantiomers by using goat serum albumin (GSA) and rabbit serum albumin (RbSA) as chiral selectors. Serum albumin (SA) was immobilized on the QCM through the self-assembled monolayer technique, and the surface concentration of GSA and RbSA were 8.8 × 10(-12) mol cm(-2) and 1.2 × 10(-11) mol cm(-2) , respectively. The QCM biosensors showed excellent sensitivity and selectivity. Meanwhile, the chiral recognition of SA sensors was quite species dependent. There were differences between GSA and RbSA sensors in the ability and the preference of chiral recognition. To R,S-1,2,3,4-tetrahydro-1-naphthylamine (R,S-1-TNA), R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA), and R,S-1-(3-methoxyphenyl)ethylamine (R,S-3-MPEA), the preference of the stereoselective SA-drug binding of the two kinds of SA sensors were consistent. However, to R,S-2-octanol (R, S-2-OT) and R,S-methyl lactate (R,S-MEL), the two kinds of SA sensors had opposite chiral recognition preference. Moreover, the interactions of SA and the five pairs of enantiomers have been further investigated through ultraviolet (UV) and fluorescent (FL) spectra. The UV/FL results were in accordance with the consequence of QCM.

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Section: Methodsmentioning

confidence: 99%

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Chen

Zhang

et al. 2012

Chirality

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“…For both sensors the highest discrimination factor were obtained for R,S-TNA. The value were for BSA sensor QCM =1.34 while for HSA sensor QCM =1.57 (Su et al 2009). …”

Section: Enantioselective Bioreceptors 41 Mass-based Biosensorsmentioning

confidence: 97%

Chiral Biosensors and Immunosensors

Trojanowicz¹,

Kaniewska²

2011

Biosensors - Emerging Materials and Applications

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“…Other chiral sensing systems combined with biomolecules have also been investigated. Ng and coworkers demonstrated real-time chiral discrimination of enantiomers of 3-methoxyphenylethylamine, tetrahydronaphthylamine, 2-octanol, and methyl lactate using a bovine serum albumin (BSA) or human serum albumin (HSA) functionalized QCM biosensor [97]. Rotello and coworkers reported protein recognition with amino acid and dipeptide-functionalized gold nanoparticles [98].…”

Section: Interfacial Techniquesmentioning

confidence: 99%

Intelligent Chiral Sensing Based on Supramolecular and Interfacial Concepts

Ariga

Richards

Ishihara

et al. 2010

Sensors

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Of the known intelligently-operating systems, the majority can undoubtedly be classed as being of biological origin. One of the notable differences between biological and artificial systems is the important fact that biological materials consist mostly of chiral molecules. While most biochemical processes routinely discriminate chiral molecules, differentiation between chiral molecules in artificial systems is currently one of the challenging subjects in the field of molecular recognition. Therefore, one of the important challenges for intelligent man-made sensors is to prepare a sensing system that can discriminate chiral molecules. Because intermolecular interactions and detection at surfaces are respectively parts of supramolecular chemistry and interfacial science, chiral sensing based on supramolecular and interfacial concepts is a significant topic. In this review, we briefly summarize recent advances in these fields, including supramolecular hosts for color detection on chiral sensing, indicator-displacement assays, kinetic resolution in supramolecular reactions with analyses by mass spectrometry, use of chiral shape-defined polymers, such as dynamic helical polymers, molecular imprinting, thin films on surfaces of devices such as QCM, functional electrodes, FET, and SPR, the combined technique of magnetic resonance imaging and immunoassay, and chiral detection using scanning tunneling microscopy and cantilever technology. In addition, we will discuss novel concepts in recent research including the use of achiral reagents for chiral sensing with NMR, and mechanical control of chiral sensing. The importance of integration of chiral sensing systems with rapidly developing nanotechnology and nanomaterials is also emphasized.

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A novel strategy for rapid real-time chiral discrimination of enantiomers using serum albumin functionalized QCM biosensor

Cited by 44 publications

References 24 publications

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Chiral Biosensors and Immunosensors

Intelligent Chiral Sensing Based on Supramolecular and Interfacial Concepts

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