A novel strategy on the high-cell-density cultivation of Candida utilis for the enhanced production of glutathione

Nie, Min; Wei, Gongyuan; Shao, Ning; Ge, Xianping

doi:10.1007/s11814-010-0190-y

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…Specifically, glutathione plays an important role in maintaining the normal cellular redox environment [4], which results in its wide utilization in the medicine, food and the cosmetic industries. Therefore, the commercial demand for glutathione keeps rapidly expanding [5].…”

Section: Introductionmentioning

confidence: 99%

“…The fermentative production of glutathione using sugar materials is regarded as one of the most promising methods, and yeasts such as S. cerevisiae and C. utilis are the commonly used microorganisms [5]. Belonging to the Crabtree negative [6] and Kluyver positive yeast [7], C. utilis possesses very weak catabolic repression effect when sugars are used as substrate.…”

Section: Introductionmentioning

confidence: 99%

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Efficient production of glutathione using hydrolyzate of banana peel as novel substrate

Chen

Wei

Zhang

et al. 2011

Korean J. Chem. Eng.

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The hydrolyzate of banana peels containing abundant fermentable sugars as glucose, xylose, mannose, and arabinose was successfully used as a novel substrate for the efficient production of glutathione by Candida utilis SZU 07-01. Xylose was first selected as the sole carbon source for glutathione production, medium optimization for better cell growth and higher glutathione using response surface methodology consisting of PB design, the steepest ascent experiment and CCD was carried out, and the optimal combination of nutrients was obtained as follows: xylose 20 g/L, (NH 4 ) 2 SO 4 9.59 g/L, KH 2 PO 4 3 g/L, L-methionine 5.72 g/L and MgSO 4 0.20 g/L. The maximum dry cell weight and glutathione achieved using the optimized medium were 7.36 g/L and 154.32 mg/L, respectively. Following with the content in this medium, other sugars like glucose, mannose and arabinose were chosen as the sole carbon source and all tested available for glutathione production. Based on these results, the hydrolyzate of banana peels was selected as a novel substrate, and a high DCW of 7.68 g/L and glutathione yield of 111.33 mg/L were obtained with the initial sugar concentration of 20 g/L in the hydrolyzate of banana peels.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient production of glutathione using hydrolyzate of banana peel as novel substrate

Chen

Wei

Zhang

et al. 2011

Korean J. Chem. Eng.

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“…1 (a)). Both variables were decreased at glucose concentrations higher than 4%, probably because the cell growth was inhibited by high osmotic pressure [ 19 , 20 ]. Thus, glucose was used as a carbon source for P-B CCRD.…”

Section: Resultsmentioning

confidence: 99%

Optimization of glutathione production in Saccharomyces cerevisiae HBSD-W08 using Plackett–Burman and central composite rotatable designs

Shen

Zhu

et al. 2023

BMC Microbiol

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Background Glutathione is an important bioactive tripeptide and is widely used in the food, medicine, and cosmetics industries. The aim of this study was to provide an efficient method for producing GSH and to explore its synthesis mechanism. Saccharomyces cerevisiae strain HBSD-W08 was screened for GSH production, and its fermentation medium was optimized using single-factor experiments of the Plackett-Burman and central composite rotatable designs. This method was used to analyze the effects of the presence and concentration of various carbon sources, organic and inorganic nitrogen sources, metal ions, and precursor amino acids on GSH production and catalase, superoxide dismutase, and γ-glutamylcysteine synthetase activity. Results The three most significant factors affecting GSH production were peptone (optimal concentration [OC]: 2.50 g L− 1), KH2PO4 (OC: 0.13 g L− 1), and glutamic acid (OC: 0.10 g L− 1). GSH productivity of HBSD-W08 was obtained at 3.70 g L− 1 in the optimized medium. The activity of γ-GCS, which is a marker for oxidative stress, was found to be highly positively correlated with GSH production. Conclusions This finding revealed an underlying relationship between GSH synthesis and oxidative stress, providing useful information for developing effective GSH fermentation control strategies.

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“…The commercial Se-enriched S. cerevisiae (2000 μg/g Se and 5.0 mg/g GSH) was purchased from AngelYeast Co., Ltd. (Yichang, China). The cells of C. utilis (14.6 mg/g GSH) and Se-enriched C. utilis (1884 μg/g Se and 13.1 mg/g GSH) were prepared using C. utilis SZU 07-01 according to the methods described in our previous studies (Wang et al, 2012;Nie et al, 2010). All the yeast cells supplied to the diet were in live conditions.…”

Section: Selenium Sourcesmentioning

confidence: 99%

Improved Antioxidant Capacity and Immune Function of Broiler Chickens Fed with Selenium-enriched Candida utilis

Wang

Wei

2020

Braz. J. Poult. Sci.

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The aim of this study was to assess the effect of selenium-enriched Candida utilis with high contents of organic selenium (Se) and glutathione (GSH) on growth performance, antioxidant capacity and immune function of broiler chickens. A total of 100 healthy 7-dayold male broiler chickens were randomly divided into 5 groups, and fed diets supplemented with (a) Na 2 SeO 3 , (b) C. utilis, (c) Se-enriched Saccharomyces cerevisiae, (d) Se-enriched C. utilis, and (e) the control without any supplements. The experiment lasted for 6 weeks and parameters were recorded on day 42. No significant differences in average daily gain were found among the 5 groups. However, Se-enriched C. utilis supplemented in the diet increased activities of glutathione peroxidase in the whole blood (p<0.01), catalase in the serum (p<0.01) and breast meat (p<0.01), and superoxide dismutase in the breast meat (p<0.01), as well as decreased contents of malondialdehyde in the serum (p<0.01), liver (p<0.01) and breast meat (p<0.05). Also, Seenriched C. utilis improved titers of IgG (p<0.01), IgM (p<0.01), and IgA (p<0.01) in the serum, as compared to the control. All these results indicated that Se-enriched C. utilis was a good candidate of dietary supplement to improve the antioxidant capacity and immune function of broiler chickens.

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A novel strategy on the high-cell-density cultivation of Candida utilis for the enhanced production of glutathione

Cited by 10 publications

References 21 publications

Efficient production of glutathione using hydrolyzate of banana peel as novel substrate

Efficient production of glutathione using hydrolyzate of banana peel as novel substrate

Optimization of glutathione production in Saccharomyces cerevisiae HBSD-W08 using Plackett–Burman and central composite rotatable designs

Improved Antioxidant Capacity and Immune Function of Broiler Chickens Fed with Selenium-enriched Candida utilis

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