A Novel Suspended Hydrogel Membrane Platform for Cell Culture

Chen, Yong X.; Yang, Shihao; Yan, Jiahan; Hsieh, Ming Han; Weng, Lingyan; Ouderkirk, Jessica L.; Krendel, Mira; Soman, Pranav

doi:10.1115/1.4031467

Cited by 6 publications

(8 citation statements)

References 37 publications

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“…1G), a correlation supported in previous work [34,37]. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 13…”

Section: Poly(aniline) Synthesis and Materials Characterizationsupporting

confidence: 73%

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Sawyer

Dong

Venn

et al. 2017

Biomed. Phys. Eng. Express

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New conductive hydrogels with superior biocompatibility continue to be developed in order to serve as bioactive scaffolds capable of modulating cellular functionality for tissue engineering applications. We developed an electrically conductive gelatin methacrylate-poly(aniline) (GelMA-PANi) hydrogel that is permissive of matrix mineralization by encapsulated osteoblast-like cells. Incorporation of PANi clusters within the GelMA matrix increases the electro-conductivity of the composite gel, while maintaining the osteoid-like soft mechanical properties that allows three-dimensional encapsulation of living cells. Viability of human osteogenic cells encapsulated within GelMA-PANi hydrogels was similar to that of GelMA. Cells within GelMA-PANi also demonstrated the capability of depositing mineral within the hydrogel matrix after being chemically induced for two weeks, although the total mineral content was lower as compared to GelMA. Additionally, we demonstrated that the GelMA-PANi-composite hydrogel could be printed in complex, user-defined geometries using digital projection stereolithography.

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Section: Poly(aniline) Synthesis and Materials Characterizationsupporting

confidence: 73%

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Sawyer

Dong

Venn

et al. 2017

Biomed. Phys. Eng. Express

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“…For this work, GelMA was chosen as our model hydrogel to encapsulate cells as it is a collagen derivative which possess RGD groups necessary for cell adhesion, contains readily tunable mechanical properties, and exhibits high structural integrity for experiments lasting over one month [41,42]. Additionally, previous work has shown that GelMA properties such as diffusion, swelling, compressive moduli, and degree of crosslinking are highly consistent between batches, allowing for the reduction of outside variables when conducting experiments [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Perfusion directed 3D mineral formation within cell-laden hydrogels

Sawyer¹,

Shridhar²,

Zhang³

et al. 2018

Biofabrication

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Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol pipes were used to create perfusable channels. A custom-built bioreactor was used to perfuse osteogenic media directly through the channels in order to induce mineral deposition which was subsequently quantified via micro-CT. Histological staining was used to verify mineral deposition around the perfused channels, while COMSOL modeling was used to simulate oxygen diffusion between adjacent channels. This information was used to design a scaled-up construct containing a 3D array of perfusable channels within cell-laden GelMA. Progressive matrix mineralization was observed by cells surrounding perfused channels as opposed to random mineral deposition in static constructs. Micro-CT confirmed that there was a direct relationship between channel mineralization within perfused constructs and time within the bioreactor. Furthermore, the scalable method presented in this work serves as a model on how large-scale bone tissue replacement constructs could be made using commonly available 3D printers, sacrificial materials, and hydrogels.

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“…For all constructs, strain percentage was increased from 0 to 40% with a preloaded force of 0.01 N and displacement of 10 lm. The slope of the stress-strain curve from 0 to 10% strain was used to report the compressive modulus as per previous protocols studying GelMA constructs 32 (n = 3 for each time point).…”

Section: Compression Modulusmentioning

confidence: 99%

Measuring Changes in Electrical Impedance During Cell-Mediated Mineralization

Ramos¹,

Zhang²,

Quinn³

et al. 2019

Bioelectricity

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Background: The fundamental electrical properties of bone have been attributed to the organic collagen and the inorganic mineral component; however, contributions of individual components within bone tissue toward the measured electrical properties are not known. In our study, we investigated the electrical properties of cell-mediated mineral deposition process and compared our results with cell-free mineralization. Materials and Methods: Saos-2 cells encapsulated within gelatin methacrylate (GelMA) hydrogels were chemically stimulated in osteogenic medium for a period of 4 weeks. The morphology, composition, and mechanical properties of the mineralized constructs were characterized using bright-field imaging, scanning electron microscopy (SEM) energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy (FITR), nuclear magnetic resonance spectroscopy (NMR), micro-CT, immunostaining, and mechanical compression tests. In parallel, a custom-made device was used to measure the electrical impedance of mineralized constructs. All results were compared with cell-free GelMA hydrogels mineralized through the simulated body fluid approach. Results: Results demonstrate a decrease in the electrical impedance of deposited mineral in both cell-mineralized and cell-free mineralized samples. Conclusions: This study establishes a model system to investigate in vivo and in vitro mineralization processes.

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A Novel Suspended Hydrogel Membrane Platform for Cell Culture

Cited by 6 publications

References 37 publications

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Perfusion directed 3D mineral formation within cell-laden hydrogels

Measuring Changes in Electrical Impedance During Cell-Mediated Mineralization

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