A novel systemic granulomatous inflammatory disease in farmed Atlantic cod, <i>Gadus morhua</i> L., associated with a bacterium belonging to the genus <i>Francisella</i>

Olsen, Anne Berit; Mikalsen, Jarle; Rode, Marit; Alfjorden, Anders; Hoel, E; Straum-Lie, K; Haldorsen, R N; Colquhoun, Duncan J.

doi:10.1111/j.1365-2761.2006.00714.x

Cited by 109 publications

(138 citation statements)

References 8 publications

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“…Multinucleated giant cells (MGC) have been described in fish with bacteriosis (Olsen et al, 2006;Swaim et al, 2006;Jacobs et al, 2009), parasitosis (Adriano et al, 2005Hogge et al, 2008), and fungus (Sosa et al, 2007;Oidtmann et al, 2008), with in Pesq. agropec.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo

Moraes

Soares

et al. 2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effect of food supplementation with vitamin C on macrophage and multinucleated giant cell (MGC) activities of pacus at two stocking densities. The experiment was carried out in a 2x2x3 split-plot factorial arrangement with: 0 and 500 mg kg -1 vitamin C; 5 and 20 kg m -3 stocking densities; and evaluation times at 3, 6, and 12 days after the subcutaneous implantation of glass coverslips (DPI). The number of macrophages and MGC, as well as cortisol and glucose plasma levels were determined. The number of macrophages and MGC with two to five nuclei was significantly greater in fish supplemented with vitamin C at 5 kg m -3 stocking density at 3 DPI in comparison to nonsupplemented ones. The macrophage and MGC counts were lower in fish with high-plasma cortisol concentration. Supplementation with 500 mg vitamin C benefits macrophage activity on foreign-body inflammation, and high-cortisol concentration has suppressive effects on this response.Index terms: Piaractus mesopotamicus, ascorbic acid, glucocorticoid, inflammation, Langhans cells, multinucleated giant cells. Vitamina C e cortisol endógeno em resposta inflamatória tipo corpo estranho em pacusResumo -O objetivo deste trabalho foi avaliar o efeito da suplementação com vitamina C no aumento da atividade de macrófagos e células gigantes multinucleadas (CGM), em pacus mantidos em duas densidades de estocagem. O experimento foi realizado em parcelas subdivididas, em arranjo fatorial 2x2x3 com: 0 e 500 mg kg -1 de vitamina C; densidade de estocagem de 5 e 20 kg m -3 ; e tempos de avaliação de 3, 6 e 12 dias após o implante subcutâneo (DPI) de lamínulas de vidro. Foram determinados o número de macrófagos e de CGM, bem como os níveis de cortisol e glicose no plasma. O número de macrófagos e de CGM com 2 a 5 núcleos foi significativamente maior nos peixes suplementados com vitamina C, à densidade 5 kg m -3 , aos 3 DPI, em relação aos não suplementados. Constatou-se menor quantidade de macrófagos e CGM em peixes com baixa concentração plasmática de cortisol. A suplementação com 500 mg de vitamina C por quilograma de ração beneficia a atividade de macrófagos em inflamações do tipo corpo estranho, e a elevada concentração de cortisol circulante tem efeito supressor nesta resposta.Termos para indexação: Piaractus mesopotamicus, ácido ascórbico, glicocorticoide, inflamação, células de Langhans, células gigantes multinucleadas.

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Section: Introductionmentioning

confidence: 99%

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo

Moraes

Soares

et al. 2012

Pesq. agropec. bras.

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“…Multinucleated giant cells (MGC) have been described among fish with infectious or parasitic diseases (Olsen et al, 2006;Hogge et al, 2008;Jacobs et al, 2009), and in experimental in vitro (Couso et al, 2002) and in vivo (Petric et al, 2003b;Belo et al, 2005) models. Accumulation of inflammatory cells at the lesion site is the main inflammation event to protect the organism against foreign elements (Kumar et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids

Sakabe

Moraes

Belo

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n-6) and linseed oil (LO, source of omega 3, n-3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n-3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body.Index terms: Oreochromis niloticus, foreign body, growth performance, inflammatory response, lipid nutrition. Cinética da inflamação crônica em tilápia-do-nilo alimentada com ácidos graxos essenciais n-3 e n-6Resumo -O objetivo deste trabalho foi investigar o efeito da suplementação alimentar com ácidos graxos essenciais sobre a cinética do acúmulo de macrófagos e a formação de células gigantes em tilápia-do-nilo (Oreochromis niloticus). As fontes de suplementação foram óleo de soja (OS, fonte de ômega 6, n-6) e óleo de linhaça (OL, fonte de ômega 3, n-3), nas seguintes proporções: 100% OS; 75% OS + 25% OL; 50% OS + 50% OL; 25% OS + 75% OL; e 100% OL (quatro repetições por tratamento). Após período de alimentação de três meses, foi avaliado o desempenho produtivo, e lamínulas de vidro foram implantadas no tecido subcutâneo dos peixes, as quais foram removidas para exame aos 2, 4, 6 e 8 dias após o implante. O desempenho produtivo não diferiu entre os tratamentos. Os peixes alimentados com 100% de óleo de linhaça tiveram maior acúmulo de macrófagos e formação mais rápida de células de Langhans, no sexto dia. No oitavo dia, as células de Langhans foram predominantes nas lamínulas implantadas nos peixes alimentados com 75 e 100% de óleo de linhaça. Os ácidos graxos n-3 podem contribuir para o recrutamento de macrófagos e a formação de células gigantes, na resposta inflamatória crônica a corpo estranho em peixes.Termos para indexação: Oreochromis niloticus, corpo estranho, desempenho produtivo, resposta inflamatória, nutrição lipídica.

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“…In spite of the limited number of the isolates tested (only one) it seems that Francisella sp., an intracellular facultative microorganism, had the highest resistance to Duplalim ® than the extracellular pathogens such as V. ordalii and V. anguillarum. In a recent study, Verner-Jeffreys et al (2009) reported that two Gram-positive bacteria (Lactococcus garviae and Carnobacterium piscicola) were apparently more resistant to four biocide products than other two of infected host cells (Olsen et al 2006) the resistance shown by the isolate to Duplalim ® might be explained by the low concentration that can be attained by this disinfectant compound. Until now, the molecular base of this resistance is unknown.…”

Section: Resultsmentioning

confidence: 99%

“…cultivated at 20 ºC were picked up from cysteine heart agar (Difco™) plates containing 5% sheep blood (Olsen et al 2006), adjusted in sterile freshwater to contain 3 x 10 8 CFu x ml -1 . Duplalim ® was evaluated during 2, 5 and 15 min treatment periods for the control of Francisella sp.…”

Section: Stage I: In Vitro Assays In Laboratorymentioning

confidence: 99%

Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile

2011

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Efficacy of a commercial disinfectant against REsuMENEn el presente trabajo se evaluó la eficacia in vitro del desinfectante Duplalim ® , una combinación sinérgica de glutaraldehído y sales de amonio cuaternario de cuarta generación, contra 4 patógenos de peces prevalentes de la salmonicultura chilena. Los resultados muestran que todas las concentraciones ensayadas (diluciones entre 1:200 a 1:400) fueron eficaces sobre los aislados de Vibrio ordalii y Vibrio anguillarum post-30 s de exposición, detectando niveles de reducción igual a 1.8 x 10 6 uFC/ ml. Concentraciones superiores de Duplalim ® (dilución 1:50) y un tiempo de exposición no menor a 5 min. fueron necesarios para eliminar completamente al patógeno intracelular Francisella sp. Cuando el desinfectante fue ensayado contra el Virus de la necrosis Pancreática infecciosa (iPnV), se detectó que la dilución 1:400 tiene un efecto significativo después de 2 minutos sin importar los títulos de iPnV testeados (mayor concentración evaluada 10 7.6 TCiD 50 / ml). Duplalim ® se evaluó en condiciones masivas contra los miembros de la familia Vibrionaceae. En comparación a los controles (sin adición desinfectante), la dilución 1:400 de Duplalim ® eliminó completamente V. ordalii y V. anguillarum después de 15 minutos de tratamiento, tanto en el agua de cultivo como en la superficie de mallas usadas en el cultivo del salmón. así, el análisis microbiológico del agua de los controles mostró concentraciones de 1.4 ± 0.3 × 10 6 uFC/ ml, mientras en el caso de las mallas 7.6 ± 3.2 × 10 5 uFC/ ml 1 . En resumen, los antecedentes obtenidos indican que el uso del desinfectante Duplalim ® es efectivo contra V. ordalii, V. anguillarum y iPnV en bajas concentraciones y cortos periodos de exposición (dilución 1:400 por 15 min.), mientras que para el patógeno intracelular se requiere una concentración mayor.

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A novel systemic granulomatous inflammatory disease in farmed Atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella

Cited by 109 publications

References 8 publications

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids

Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile

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