A novel technique for NACE coupled with simultaneous electrochemiluminescence and electrochemical detection for fast analysis of tertiary amines

Abstract

A simultaneous electrochemiluminescence (ECL) and electrochemical (EC) detection scheme for NACE was presented for fast analysis of tertiary amines. Both ECL and EC signals were generated at the same Pt electrode. Triethylamine (TEA), tripropylamine (TPrA), chlorpromazine, promethazine, and dioxopromethazine (DPZ) were selected to validate NACE-ECL/EC dual detection strategy. The linear ranges for TEA and TPrA were 0.01-500 and 0.01-10 microM with the detection limits of 8.0 and 5.0 nM (S/N=3), respectively. The RSDs (n=6) of the migration time and the ECL intensity for 1 microM TEA and 0.5 microM TPrA were 0.1 and 2.8%, and 0.2 and 1.8% with theoretical plate numbers of 180,000 and 700,000 per meter, respectively. These two analytes could be separated within 92 s and the Pt electrode did not need reactivation during the experiments. Chlorpromazine, promethazine, and DPZ could be well separated by NACE. The proposed method was also demonstrated for fast determination of DPZ in human urine with simple sample preparation. The results indicated that NACE-ECL/EC had the advantages of simple and fast analysis with more information, wide linear range, high sensitivity, and compatibility with real urine sample.

Determination of enoxacin and ofloxacin by capillary electrophoresis with electrochemiluminescence detection in biofluids and drugs and its application to pharmacokinetics

“…Recently, CE-CL has been widely developed and become a useful tool for rapid detection of various analytes such as metal ions (Ren and Huang, 2001), amino acids , proteins (Zhi et al, 2007) and antigen-antibody complexes (Wang and Ren, 2005). The marriage of CE to ECL is a sensitive and effi cient analytical technique and has got excellent performance for the analysis of proteins Guo et al, 2009), amino acids (Li et al, 2006) and drugs (Zhao et al, 2004;Yuan et al, 2009;Liu et al, 2008aLiu et al, , 2009aXu et al, 2006;Deng et al, 2008).…”

Some important combinations of detection techniques for electrophoresis in capillaries and on chips with emphasis on electrochemical principles

“…Dual AMP/ECL detection was also used in CZE separations in non-aqueous media (NACE) [34,35]; for a principal scheme of the detection system, see Fig. 4A, and for details, see [36].…”

“…The dual detector was evaluated in [34] on determination of triethylamine (TEA), tripropylamine (TPrA), chlorpromazine, promethazine and dioxopromethazine (DPZ). The high sensitivity of ECL detection was reflected in low detection limits, 8 and 5 nM for triethylamine and tripropylamine, respectively (these analytes do not yield amperometric signals).…”

A sensitive method for simultaneous determination of four macrolides by CE with electrochemiluminescence detection and its applications in human urine and tablets

“…Recently, ECL based on tris(2,2 0 -bipyridyl) ruthenium(II) (Ru(bpy) 3 21 ) has received considerable attention for the detection of nitrogen-containing compounds especially tertiary amines because of their good ECL performances. CE coupled with ECL using Ru(bpy) 3 21 has proved to be a sensitive and efficient analytical technique and has been successfully demonstrated in analysis of proteins [13], amino acids [14,15], quinoline [16], quinolones [17], amines [18] and alkaloids [19]. Macrolide antibiotics (e.g.…”