A novel technology for the one-step detection of prune dwarf virus: Colorimetric reverse transcription loop-mediated isothermal amplification assay

Çeli̇k, Ali

doi:10.1016/j.cropro.2022.105910

Cited by 9 publications

(1 citation statement)

References 61 publications

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“…The investigation was successful in obtaining a naked eye observation of positive LAMP reactions, where the colour changing from pink to yellowish signals a positive result of the virus infection in prunes. This method's sensitivity was 10 times more than that of traditional PCR ( Çelik, 2022 ). Another team of researchers developed a LAMP assay to identify the pea enation mosaic virus (PEMV), which infects plants belonging to Leguminosae, using specific primer sets as inner- and outer primers.…”

Section: Modern Biosensor Technology For Plant Pathogen Detectionmentioning

confidence: 98%

A review of recent advances in plant-pathogen detection systems

Patel¹,

Mitra²,

Vinchurkar³

et al. 2022

Heliyon

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Section: Modern Biosensor Technology For Plant Pathogen Detectionmentioning

confidence: 98%

A review of recent advances in plant-pathogen detection systems

Patel¹,

Mitra²,

Vinchurkar³

et al. 2022

Heliyon

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Wild Relatives and Their Contributions to Wheat Breeding

Tekin

Emi̇rali̇oğlu²,

Yeken³

et al. 2022

Ancient Wheats

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European and American grapevines were successfully recovered from GFkV, GLRaV1, GLRaV2, and GLRaV3 viruses by a modified thermotherapy and shoot tip culture

Sağlam,

Çalkan Sağlam,

Güler

et al. 2023

Phytoparasitica

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The purpose of this study was to look into the use of thermotherapy and shoot tip culture to disinfect grapevines from Grapevine fanleaf nepovirus (GFLV), Grapevine eck virus (GFkV), and Grapevine leafroll closterovirus (GLRaV) -1,-2,-3, all of which are required for certi ed sapling production. The GFkV, GLRaV-1,2,3-infected grapevine scions were taken from the initial testing and put into 10-liter plastic containers.They were rooted, and saplings were grown from them. The grapevines were then treated to a modi ed thermotherapy treatment for 40 days, which included 8 hours of darkness at 60-70% relative humidity and 32°C temperature and 16 hours of light (4000-5000 lux) at 60-70% relative humidity and 40°C temperature. The experimental materials were treated to shoot tip culture procedures after thermotherapy.At the end of the acclimation, all materials were tested again. At the conclusion of the testing procedures, the entire factory was virus-free. The survival rates of the grapevines differed, with 41B rootstock having a 100% survival rate and the rest having at least one survived plant out of six. When paired with rapid multiplication through in-vitro propagation, the proposed thermotherapy approach used in this study has a high potential for virus-free grapevine sapling propagation.

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A novel technology for the one-step detection of prune dwarf virus: Colorimetric reverse transcription loop-mediated isothermal amplification assay

Cited by 9 publications

References 61 publications

A review of recent advances in plant-pathogen detection systems

A review of recent advances in plant-pathogen detection systems

Wild Relatives and Their Contributions to Wheat Breeding

European and American grapevines were successfully recovered from GFkV, GLRaV1, GLRaV2, and GLRaV3 viruses by a modified thermotherapy and shoot tip culture

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